广东地区水禽源大肠杆菌对β-内酰胺类药物的耐药性及耐药基因检测

doi:10.16431/j.cnki.1671-7236.2015.09.040

Abstract

Abstract: In order to understand the resistance to β-lactams antimicrobial agents and the prevalence of resistance genes, 243 Escherichia coli (E.coli) were isolated and identified from waterfowl in Guangdong province during 2012 to 2014.Drug sensitivity of total 243 E.coli isolates to 4 kinds of β-lactams antimicrobial agents were determined by double broth dilution method.All β-lactams resistant isolates were detected for the presences of bla_CTX-M, blasub>TEM and bla_OXA genes by PCR.The results showed that the occurrence of ampicillin and amoxicillin were ranked the higher (80.00% to 100.00%), and were 9.09% to 50.00% for ceftazidime and ceftiofur.The bla_CTX-M, bla_TEM and bla_OXA genes were detected in 88.46% to 90.78%, 67.21% to 88.52% and 51.28% to 59.02% of the β-lactams resistant isolates to the four kinds of antimicrobial agents, respectively.The results indicated that the resistance of E.coli isolates from waterfowl to β-lactams antimicrobial agents were widespread, and penicillin was particularly serious.The bla_CTX-M, bla_TEM and bla_OXA genes were mainly carried β-lactams resistant genes in E.coli isolates from waterfowl in Guangdong province.It had a great relationship between the prevalence of resistance genes and growth of antimicrobial resistance.

Key words: Escherichia coli from waterfowl; drug sensitivity test; β-lactams resistance genes

CLC Number:

S852.61⁺2

ZHANG Ji-pei, TAN Hua-long, WEI Qing-lan, CHEN Jian-hong. Detection of Resistance and Resistance Genes of Escherichia coli to β-lactams Antimicrobial Agents Isolated from Waterfowl in Guangdong Province[J]. , 2015, 42(9): 2487-2492.

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Detection of Resistance and Resistance Genes of Escherichia coli to β-lactams Antimicrobial Agents Isolated from Waterfowl in Guangdong Province

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