圈养老年大熊猫肠道菌群16SrDNA克隆文库的建立

doi:10.16431/j.cnki.1671-7236.2015.06.012

Abstract

Abstract: In order to comparatively analyze the differences and similarities of intestinal flora from all ages of giant pandas,we built 16S rDNA clone library of three captive elderly giant pandas'fecal bacteria and made restriction fragment length polymorphism (RFLP) and sequencing analysis by using restriction enzymes HinfⅠ and MspⅠ.The results showed that the intestinal flora of elderly giant panda were mainly attributable to two bacteria:Proteobacteria and Firmicutes.Between them,the main in Proteobacteria of intestinal flora of elderly giant panda were Escherichia coli,followed by Pseudomonas sp.,Shigella and Aeromonas;The main in Firmicutes were Streptococcus,followed by Weissella,Clostridium;Of which there were a certain proportion of bacteria were uncultured bacteria.The article firstly established a rich clone library of intestinal flora from elderly giant pandas,which provided a reference to comparatively analyze the similarities and differences in structure of intestinal flora from all ages of giant pandas,as well as provided the important information about reasonable feeding elderly giant pandas and protecting health of elderly giant pandas.

Key words: captivity; elderly giant panda; intestinal flora; 16S rDNA clone library

CLC Number:

S858.93

WANG Xiao-yan, YUAN Ting, LIAO Hong, LUO Yong-jiu, HE Ting-mei, WANG Cheng-dong, LIU Xiao-min, WU Hong-lin, PENG Guang-neng. Construction of the 16S rDNA Clone Library of Intestinal Flora of the Captive Elderly Giant Panda[J]. , 2015, 42(6): 1402-1408.

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Construction of the 16S rDNA Clone Library of Intestinal Flora of the Captive Elderly Giant Panda

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