4味常见中药对免疫抑制小鼠免疫功能及肠道菌群的影响

doi:10.16431/j.cnki.1671-7236.2019.01.017

Abstract

Abstract:

The effects of 4 kinds of traditional Chinese medicine on immune performance and intestinal flora in immunosuppressed mice were studied.70 6-week-old male Kunming mice were chosen and assigned to 7 groups:Blank control group,model group,YPF group,Fructus Mume group,Herba Taraxaci group,Codonopsis Radix group and Pericarpium Granati group.A pathological model of immunosuppression was established by cyclophosphamide (Cy).From the 3rd day of administration,75 mg/(kg·BW) Cy was intraperitoneal injected in groups 2 to 7,once every another day for 4 times.During the injection,the normal administration was continue to perform for 7 d,and 0.9% saline was administered in model group until the second day after the Cy injection was stopped.The effects of 4 traditional Chinese medicines on body weight,spleen index,thymus index,blood indicator,sIgA,IgG and intestinal flora of immunosuppressed mice were examined.The results showed that compared with model group,the body weight of immunosuppressed mice were significantly increased by Fructus Mume,Herba Taraxaci,Codonopsis Radix and Pericarpium Granati (P < 0.05),and the effect of Fructus Mume was the best.The immune organ index of immunosuppressed mice were significantly increased by 4 kinds of Chinese medicines (P < 0.05),but could not reach the normal level,of which the spleen index of Herba Taraxaci group was the highest.The WBC were improved and the RBC were increased significantly (P < 0.05),but could not reach the normal level.The concentration of immunoglobulin in each Chinese medicine group was increased,the sIgA and IgG in the Codonopsis Radix group were significantly higher than that in the blank control group (P < 0.05),buts no significant different with YPF group (P > 0.05).The number of Escherichia coli was significantly decreased (P < 0.05),the number of Bifidobacterium and Lactobacillus was significantly increased (P < 0.05) and the richness and diversity of intestinal flora of immunosuppressed mice were increased,of which the Codonopsis Radix and Herba Taraxaci groups were better.In conclusion,Fructus Mume,Herba Taraxaci,Codonopsis Radix and Pericarpium Granati could antagonize the effects of Cy on body weight,immune function and intestinal flora in mice,and could be used in the development of immune-enhancing drugs.

Key words: traditional Chinese medicine; cyclophosphamide; immunosuppressed mice; intestinal flora; immune function

CLC Number:

S853.74

GU Wei, SUN Mingjie, WANG Lirong, CHEN Zhen, ZENG Jiajia, CHEN Jing, XU Haiyan, WANG Chunfeng. Effects of Four Kinds of Traditional Chinese Medicine on Immune Performance and Intestinal Flora in Immunosuppressed Mice[J]. , 2019, 46(1): 147-156.

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Effects of Four Kinds of Traditional Chinese Medicine on Immune Performance and Intestinal Flora in Immunosuppressed Mice

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