牛多个组织microRNASolexa测序与生物信息学分析

doi:10.16431/j.cnki.1671-7236.2015.06.013

Abstract

Abstract: In order to provide more information for further studies of miRNA's biological function,the identification of microRNA of bovine was studied.A Solexa sequencing of multiple tissues from a Simmental bull and a Holstein cow was conducted,and then bioinformatics analysis was performed.Meanwhile,we randomly selected and validated 3 miRNAs (bta-miR-2346-5p,novel_miR-48,novel_miR-86) by stem-loop RT-PCR.From this data set,we identified 604 miRNAs,including 429 known miRNAs and 175 novel miRNAs.And the stem-loop RT-PCR result showed that the 3 miRNAs were ubiquitously expressed in longissimus dorsi muscle,cardiac muscle,liver,spleen,lung,kidney,large intestine and small intestine.The study enriched the amount and kinds of bovine miRNAs which provided more credible information for the miRNAs biological functions study in various species.

Key words: bovine; multiple tissues; microRNA; Solexa sequencing

CLC Number:

S858.23

LIU Fei, YANG Ji-ye, SUN Zhi-ying, HU Xin, LI Juan, KONG Ling-na, LIANG Rui-yuan, SONG Zhen-yu, ZHANG Lu-pei, CHEN Yan, GAO Xue, LI Jun-ya, SUN Shao-hua. Solexa Sequencing and Bioinformatics Analysis of microRNA from Bovine Multiple Tissues[J]. , 2015, 42(6): 1409-1416.

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Solexa Sequencing and Bioinformatics Analysis of microRNA from Bovine Multiple Tissues

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