›› 2018, Vol. 45 ›› Issue (9): 2463-2470.doi: 10.16431/j.cnki.1671-7236.2018.09.015

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Effects of Rumen-derived Probiotics on the Growth and Physiological Characteristics of Mice

JI Hailong, WANG Hongying, ZHOU Yingnan, QIAN Siriguleng   

  1. College of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China
  • Received:2018-04-04 Online:2018-09-20 Published:2018-09-26

Abstract:

The experiments of six kinds of probiotics screened from the rumen were conducted to provide references for their further development and utilization in livestock and poultry husbandry.56 male 5-week-old Kunming mice were chosen and randomly divided into 7 groups.The mice in control group was fed with 0.2 mL of normal saline,while that of the other experimental groups were fed with 0.2 mL/(10 g·BW) of Bacillus amyloliquefaciens Lw-J21,Bacillus subtilis Lw-K16,Lactobacillus Lw-R35,Bifidobacterium Lw-D48,cellulose-producing bacteria Lw-X21,Candida dattila Lw-JM70 (1×107 CFU/mL) for 42 d,respectively.The effects of different probiotics on body weight,intestinal flora,fat accumulation,immune indexes and hypoglycemia of mice were compared and analyzed.The results showed that compared with the control group,the body weight of mice in Lw-J21 and Lw-X21 groups were higher (P>0.05), and the fat accumulation in mice were significantly reduced at the same time (P<0.05).The number of Lactobacillus and total anaerobic bacteria in the intestinal tract of mice in Lw-R35 and Lw-X21 groups were significantly or extremely significantly increased (P<0.05;P<0.01).The spleen index and thymus index of mice in probiotics groups significantly or extremely significantly enhanced (P<0.05;P<0.01),meanwhile the hypoglycemia of mice was improved.In conclusion,the screed probiotics had different degrees of influence on intestinal microenvironment, immune indexes and blood glucose regulation ability of mice.

Key words: probiotic; intestinal flora; fat accumulation; immune organ indexes; blood glucose

CLC Number: