五指山猪线粒体控制区序列分析及遗传多样性研究

doi:10.16431/j.cnki.1671-7236.2015.05.029

Abstract

Abstract: In order to research the genetic diversity and phylogenies of Wuzhishan pig, 56 complete mtDNA D-Loop were analyzed.55 sequences were amplified by polymerase chain reaction (PCR) and 1 sequence was downloaded from GenBank.The results showed that repetitive sequence "TTATAAAACAC" appeared in the conservative regions of 21 mtDNA D-Loop sequences.13 haplotypes and 14 variable sites were observed in 56 sequences.Haplotype diversity and nucleotide diversity (Hd =0.838 and Pi=0.00334) indicated that Wuzhishan pig had high genetic diversity.Two branches in the phylogenetic tree and Network analysis diagram indicated that there were two maternal origins in Wuzhishan pig.The phylogenetic tree of Wuzhishan pig and other 23 species showed that Wuzhishan pig had close genetic relationship with the pig breeds in the south region of the Yangtze river.

Key words: Wuzhishan pig; mitochondrial DNA D-Loop; genetic diversity

CLC Number:

S813.3

YU Ping, CAO Ting, SHI Li-guang, LIU Ya-fang, ZHAO Chun-ping, ZHANG Li-ling, HOU Guan-yu. Complete Mitochondrial DNA D-Loop Sequence and Genetic Diversity of Wuzhishan Pig[J]. , 2015, 42(5): 1222-1231.

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Complete Mitochondrial DNA D-Loop Sequence and Genetic Diversity of Wuzhishan Pig

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