›› 2010, Vol. 37 ›› Issue (4): 143-145.

• 遗传繁育 • 上一篇    下一篇

如皋鸡EX-FABP基因遗传多态性及其与肌内脂肪含量的相关分析

周琼1,常国斌1,王克华2,窦套存2,廖菁1,陈国宏1   

  1. (1.扬州大学动物科学与技术学院, 扬州 225009; 2.中国农业科学院家禽研究所, 扬州 225003)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-04-20 发布日期:2010-04-20
  • 通讯作者: 陈国宏

tudy on Single Nucleotide Polymorphism of EX-FABP Gene and its Association with IMF in Ru-gao Chicken

ZHOU Qiong1,CHANG Guo-bin1,WANG Ke-hua2,DOU Tao-cun2,LIAO Jing1,CHEN Guo-hong1   

  1. (1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;2.Poultry lnstitute,Chinese Academy of Agricultural Sciences, Yangzhou 225003, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20
  • Contact: CHEN Guo-hong

摘要: 以60周龄和自繁后的12周龄的如皋鸡作为试验材料,采用PCR-SSCP技术对细胞外脂肪酸结合蛋白(EX-FABP)基因进行单核苷酸多态性及遗传特异性分析。结果检测到2个SNP突变,分别为4271 G/A、4281 G/A突变,有3种基因型。最小二乘差异显著性检验结果表明,在60周龄时,AA型含量最高,显著高于AB型(P<0.05);12周龄时,AB型含量最高,BB型最低;60周龄的肌内脂肪含量显著低于12周龄时的含量(P<0.05)。

关键词: 细胞外脂肪酸结合蛋白(EX-FABP); 单核苷酸多态性; 鸡; 肌内脂肪

Abstract: This study was designed to investing the single nucleotide polymorphism and analyzing the genetic specificity of the EX-FABP gene, utilizing the PCR-SSCP technology and DNA sequencing, the 60 weeks of age and reproduction of the 12-week-old Ru-gao chicken as the experimental material. It detected three genotypes, two SNP mutations, 4271 G/A,4281 G/A respectively. The results showed that the content of AA-type was the mostly at the 60-week-old, significantly higher than the AB-type(P<0.05), using the least-squares significant difference test. At the 12- week-old, the AB-type was in the highest levels, as well as the BB-type was minimum in three genotypes. The intramuscular fat content at 60-week-old was significantly lower than that of the 12-week-old(P<0.05).

Key words: extracellular fatty acid binding protein (EX-FABP); SNP; chicken; intramuscular fat

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