关键词: 唾液酸转移酶; 流感受体; 鸡; 半定量; 生物信息学

Abstract: This study was designed to analyse the tissue specific expression profile and bioinformatics characteristic of ST3Gal6 in Chinese yellow chicken. Based on the sequence of the ST3Gal6 of the chicken reported in NCBI,2 pairs of primers were designed and the primers were used to amplify the Chinese yellow chicken ST3Gal6 gene by PCR. Then the tissue specific expression profile was analyzed by semi quantitative RT-PCR,and the bioinformatics analysis of ST3Gal6 was summarized.The results showed that,the full length of the cloning gene of ST3Gal6 was 1169 bp,including the 1059 bp of CDS sequence of chicken ST3Gal6,which encoding a 352 amino acid residues deduced protein. This nucleotide sequence of CDS shared 62%,62%,61.9%,59%,54.4% homology with the ST3Gal6 mRNA from human,pongo pygmaeus,cattle,mouse,Xenopus laevis respectively.Semi quantitative PCR revealed that the ST3Gal6 gene was expressed in various tissues,but at different levels. The expression level of this gene was higher in the brain while lower in lung tissue. The biologic softwares were used to predict the bioinformatics of ST3Gal6,which showed that the ST3Gal6 was a kind of transmembrance protein and have 2 transmembrane domains in chicken.There were 22 phosphorylattion sites and one phosphory lation site of specific protein kinase in ST3Gal6 protein.

Key words: sialyltransferase; influenza receptor; chicken; semi quantitative RT-PCR; bioinformatics