›› 2008, Vol. 1 ›› Issue (4): 45-47.

• 遗传繁育 • 上一篇    下一篇

3种提取基因组DNA方法的比较分析

刘燕1,刘孟洲1,徐彦祥2   

  1. 1.甘肃农业大学动物科技学院,兰州 730070;2.兰州猪场,榆中 730100
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-04-20 发布日期:2008-04-20

Comparison of Three Methods for Genomic DNA Extraction

LIU Yan1, LIU Meng-zhou1, XU Yan-xiang2   

  1. 1.Faculty of Animal Science & Technology,Gansu Agriculture University,Lanzhou 730070,China;2.Lanzhou Swine Factory,Yuzhong 730100,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-04-20 Published:2008-04-20

摘要: 分别采用3种不同的方法提取基因组DNA,比较所提取的DNA的质量以及提取所需时间、费用,以期选择一种高效、简洁快速、价格合理的提取方法。结果发现这3种方法提取的质量都能达到相关分子生物学试验的要求,但在操作步骤、时间、价格等方面存在差异,具体比较结果可为分子生物学试验选择提取方法提供依据。

关键词: DNA提取; 血液; 组织; FTA采样卡

Abstract: We separately used several different method to extracted genomic DNA,
compared with several methods as well as the time, the expense and the time to choose an efficient, simple fast, reasonably priced extraction methods. Finally: Several methods could achieve the quality of molecular biology experiments related requirements, but in the sequence of operation, the time and the price there exist differences.Specific results of this comparison to be possible to provide the basis for the molecular biology experiment selective extraction method.

Key words: DNA; extraction; blood; organizations; FTA sampling card

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