水牛KLF15基因克隆、分子特征和组织差异表达分析

doi:10.16431/j.cnki.1671-7236.2024.09.002

摘要/Abstract

摘要： 【目的】试验旨在获取水牛Krüppel样因子15(Krüppel-like factor 15,KLF15)基因，分析其分子特征和组织差异表达，初步揭示该基因的结构和功能。【方法】克隆水牛KLF15基因编码区，对该基因的结构、氨基酸序列一致性、理化特性、基序、保守结构域、生物学功能等进行比较分析，并采用实时荧光定量PCR法检测KLF15基因在水牛各组织中的表达水平。【结果】克隆得到水牛KLF15基因编码区长为1 212 bp，编码403个氨基酸。生物信息学分析显示，水牛KLF15与其他牛科物种氨基酸序列的一致性较高，是核内的亲水性蛋白，无信号肽序列及跨膜结构，包含KLF15_N、COG5048和zf-C2H2锌指结构等保守结构域。水牛KLF15的主要功能是调控DNA转录、结合DNA、结合锌指结构等。KLF15基因在泌乳期水牛的脂肪中表达量最高，在非泌乳期水牛的肌肉中表达量最高，但在2个时期的水牛中均为在乳腺、心脏、肝脏、脾脏等组织中中度表达，在肺脏、肾脏、瘤胃和小肠等组织中低表达或几乎不表达。【结论】水牛KLF15是细胞核内的蛋白，可结合锌指结构调控DNA的转录，在脂合成旺盛的组织中发挥功能。

关键词: 水牛; KLF15基因; 分子特征; 组织表达; 生物信息学

Abstract: 【Objective】 The aim of this study was to obtain Krüppel-like factor 15 (KLF15) gene in buffalo, analyze its molecular characteristics and tissue differential expression, and initially elucidate the structure and function of this gene. 【Method】 The CDS region of KLF15 gene in buffalo was cloned, and comparative analysis were performed on gene structure, amino acid sequence identity, physicochemical properties, motifs, conserved domains, biological functions, and so on.The expression of KLF15 gene in various tissues of buffalo were detected by Real-time quantitative PCR. 【Result】 The length of KLF15 gene CDS in buffalo was 1 212 bp, encoding 403 amino acids.Bioinformatics analysis showed that buffalo KLF15 had a high sequence identity with other bovine species，it was a hydrophilic protein in nucleus with no signal peptide or transmembrane helix, which contained conserved domains of the KLF15_N, COG5048 and zf-C2H2 zinc finger structures.Buffalo KLF15 was mainly regulate DNA transcription, bind DNA, bind zinc finger structure.KLF15 gene was most highly expressed in fat of lactating buffalo while in muscle of non-lactating buffalo.In both lactating and non-lactating buffaloes, KLF15 gene was moderately expressed in mammary gland, heart, liver and spleen, while low or almost no expression was observed in lung, kidney, rumen and small intestine. 【Conclusion】 Buffalo KLF15 was a protein localised in nucleus that binding to zinc finger structures to regulate DNA transcription, which played a role in tissues with vigorous lipid synthesis.

Key words: buffalo; KLF15 gene; molecular characteristics; tissues expression; bioinformatics

中图分类号:

周芳廷. 水牛KLF15基因克隆、分子特征和组织差异表达分析[J]. 中国畜牧兽医, 2024, 51(9): 3715-3725.

ZHOU Fangting. Cloning,Molecular Characteristics and Tissue Differential Expression Analysis of KLF15 Gene in Buffalo[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(9): 3715-3725.

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