延边黄牛lncRNA MEG3基因多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.12.025

摘要/Abstract

摘要： 试验旨在研究长链非编码RNA(long noncoding RNA,lncRNA)MEG3(maternally expressed gene 3)基因单核苷酸多态性(single nucleotide polymorphism,SNP)及其与延边黄牛生长性状之间的关联性。通过DNA直接测序法和高分辨率熔解曲线技术(high resolution melting curve,HRM)分析了90头24月龄延边黄牛MEG3基因多态性,并结合胸宽、髋宽和腹围等13项生长性状进行关联分析。结果显示,MEG3基因第5外显子包含g.65745819 G>A和g.65745850 C>A 2处突变,均存在2个等位基因,分别为G、A和C、A,等位基因频率分别为0.661、0.339和0.778、0.222,产生3个基因型,分别为GG、GA、AA和CC、CA、AA,基因型频率分别为0.422、0.478、0.100和0.611、0.333、0.056;卡方适应性检验发现,2个位点均未偏离哈迪-温伯格平衡(P>0.05)。2个位点遗传纯合度、杂合度、有效等位基因数和多态信息含量(PIC)分别为0.552、0.448、1.812、0.348和0.286、0.346、1.528、0.286,均处于中度多态(0.25<PIC<0.5)。关联分析显示,g.65745819 G>A位点GA基因型胸宽显著高于AA基因型(P<0.05),AA基因型髋宽显著高于GG和GA基因型(P<0.05);g.65745850 C>A位点AA基因型腹围显著高于CA基因型(P<0.05),其余基因型各指标之间差异均不显著(P>0.05)。结果表明,lncRNA MEG3基因多态性与延边黄牛部分生长性状相关,可作为延边黄牛分子标记辅助育种的候选基因。

关键词: 延边黄牛; lncRNA; MEG3基因; SNP; 生长性状

Abstract: The aim of this study was to investigate the association between long noncoding RNA (lncRNA) maternally expressed gene 3 (MEG3) gene single nucleotide polymorphism (SNP) and the growth traits in Yanbian Yellow cattle.DNA direct sequencing and high resolution melting curve (HRM) were used to analyze the polymorphism of MEG3 gene in 90 Yanbian Yellow cattle at 24 months of age, and association analysis was performed combined with 13 growth traits such as chest width, thurl width and perimeter of abdomen.The results showed that MEG3 gene exon 5 contained two mutations of g.65745819 G>A and g.65745850 C>A, both of them had two alleles (G, A and C, A), the allele frequency were 0.661, 0.339 and 0.778, 0.222, respectively, and produced three genotypes(GG, GA, AA and CC, CA and AA), the genotype frequency were 0.422, 0.478, 0.100 and 0.611, 0.333, 0.056, respectively.χ² test showed no deviation from Hardy-Weinberg equilibrium (P>0.05).The genetic homozygosity, heterozygosity, effective number of alleles and polymorphism information content (PIC) of the two loci were 0.552, 0.448, 1.812, 0.348 and 0.286, 0.346, 1.528, 0.286, respectively, which were in moderate polymorphism (0.25<PIC<0.5).Association analysis showed that the chest width of GA genotype at g.65745819 G>A was significantly higher than that of AA genotype (P<0.05), and the thurl width of AA genotype was significantly higher than that of GG and GA genotypes (P<0.05).The perimeter of abdomen trait of AA genotype at g.65745850 C>A was significantly higher than that of CA genotype (P<0.05), but there was no significant difference among other genotypes and traits (P>0.05).The results indicated that the polymorphism of lncRNA MEG3 gene was associated with some growth traits of Yanbian Yellow cattle, and could be used as a candidate gene for molecular marker assisted breeding of Yanbian Yellow cattle.

Key words: Yanbian Yellow cattle; lncRNA; MEG3 gene; SNP; growth traits

中图分类号:

S813.3

田佺, 赵祎, 王卓, 刘磊, 张君正, 戢爽. 延边黄牛lncRNA MEG3基因多态性及其与生长性状的关联分析[J]. 中国畜牧兽医, 2021, 48(12): 4567-4574.

TIAN Quan, ZHAO Yi, WANG Zhuo, LIU Lei, ZHANG Junzheng, JI Shuang. Polymorphisms of lncRNA MEG3 Gene and Its Correlation with Growth Traits in Yanbian Yellow Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4567-4574.

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