大白猪BMPR-ⅠB基因多态性及其与繁殖性状的相关性研究

doi:10.16431/j.cnki.1671-7236.2018.04.019

《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (4): 984-991.doi: 10.16431/j.cnki.1671-7236.2018.04.019

大白猪BMPR-ⅠB基因多态性及其与繁殖性状的相关性研究

胡慧艳¹, 贾青^1,2, 侯胜奎¹, 刘津¹, 刘洋洋³, 张伟峰⁴, 赵坤峰⁵, 王贵江⁶

1. 河北农业大学动物科技学院, 保定 071000;
2. 国家北方山区农业工程技术研究中心, 保定 071000;
3. 河北顺德天兆畜牧科技有限公司, 万全 076250;
4. 河北工程大学生命科学与食品工程学院, 邯郸 056000;
5. 河北东旺种猪繁育有限公司, 宁晋 055550;
6. 河北省畜牧良种工作站, 石家庄 050061

收稿日期:2017-10-23 出版日期:2018-04-20 发布日期:2018-04-25
通讯作者: 贾青(1964-),男,博士,教授,博士生导师,研究方向:动物遗传育种,E-mail:jiaqing@hebau.edu.cn E-mail:jiaqing@hebau.edu.cn
作者简介:胡慧艳(1989-),女,河北石家庄人,博士生,研究方向:动物遗传育种与繁殖,E-mail:huhuiyan315@163.com
基金资助:
河北省研究生创新资助项目（CXZZBS2017068）；河北省现代农业产业技术体系专项（HBCT2013070202）

Study on Polymorphism of BMPR-ⅠB Gene and Its Correlation with Reproductive Traits in Large White Pig

HU Huiyan¹, JIA Qing^1,2, HOU Shengkui¹, LIU Jin¹, LIU Yangyang³, ZHANG Weifeng⁴, ZHAO Kunfeng⁵, WANG Guijiang⁶

1. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China;
2. National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071000, China;
3. Hebei Shunde Tianzhao Livestock Technology Co., Ltd., Wanquan 076250, China;
4. School of Life Science and Food Engineering, Hebei University of Engineering, Handan 056000, China;
5. Hebei Dongwang Breeding Co., Ltd., Ningjin 055550, China;
6. Animal Husbandry and Breeding Station of Hebei, Shijiazhuang 050061, China

Received:2017-10-23 Online:2018-04-20 Published:2018-04-25

摘要/Abstract

摘要：

试验旨在研究不同品系大白猪骨形态发生蛋白受体ⅠB（bone morphogenetic protein receptor-ⅠB，BMPR-ⅠB）基因多态性及其与繁殖性状的相关性。采用Hi-SNP中高通量基因分型技术对BMPR-ⅠB基因多态位点进行分型，利用SPSS统计软件，采用最小二乘法将各位点不同基因型与总产仔数、产活仔数、死胎数、畸形头数进行相关性分析。结果显示，不同品系大白猪在BMPR-ⅠB基因746A > G和852G > A位点均未检测到多态性，804G > C位点上均表现为GG基因型为优势基因型，G为优势等位基因；960C > T位点在美系大白猪中以CC基因型频率较高，而在英系大白猪中以TT基因型频率较高，在加系大白猪中CT基因型频率较高，3个不同品系大白猪群体在804G > C和960C > T位点均处于Hardy-Weinberg平衡状态（P > 0.05）。804G > C位点在3个不同品系大白猪中对总产仔数、产活仔数、死胎数、畸形数等繁殖性状均无显著影响（P > 0.05）。960C > T位点在美系大白猪群体中TT基因型死胎数极显著低于CC基因型1.027头（P < 0.01）；英系大白猪群体中TT基因型总产仔数显著高于CC基因型1.976头（P < 0.05），极显著高于CT基因型2.118头（P < 0.01）；加系大白猪中TT基因型产活仔数显著高于CC基因型0.886头（P < 0.05），死胎数、畸形头数显著低于CC基因型0.707和0.337头（P < 0.05）。960C > T位点中TT基因型可作为3个不同品系大白猪繁殖性状的标记基因型。

关键词: 大白猪; BMPR-ⅠB基因; 繁殖性状; 关联分析

Abstract:

This study was aimed to investigate the polymorphism of bone morphogenetic protein receptor-ⅠB (BMPR-ⅠB) gene and its correlation with the reproductive traits in Large White pigs.The polymorphism of BMPR-ⅠB gene was analyzed by high-throughput sequencing technologiy,the association of different genotypes of BMPR-ⅠB gene with the total number of births,alive births,deaths and deformities using the least square method by SPSS statistical software.The results showed that the polymorphism was not detected in BMPR-ⅠB gene 746A > G and 852G > A loci,in the 804G > C locus,the frequency of GG genotype was dominant genotype,and G was the dominant allele; In the 960C > T locus,the frequency of genotypes CC,TT and CT were higher in American,British and Canadian Large White pigs,respectively.The results of χ² test showed that the 804G > C and 960C > T loci were fit with Hardy-Weinberg equilibrium in the different Large White pigs lines (P > 0.05).There were no significant differences in the effects of 804G > C locus on the number of total births,alive births,still births,deformity and other reproductive traits of the three different lines of Large White pigs (P > 0.05).The number of stillbirths with TT genotype in American Large White pigs were significantly lower than that of CC genotype in 960C > T locus (P < 0.05),which was decreased by 1.027; The total litter size of the British Large White pigs with TT genotype was significantly higher than that of CC genotype (P < 0.05),and extremely significantly higher than that of CT genotype (P < 0.01),which was in-creased 1.976 and 2.118,respectively; The number of live births in Canadian Large White pigs with TT genotype was significantly higher than that of CC genotype (P < 0.05),the stillbirth and deformity of TT genotype were significantly lower than that of CC genotype (P < 0.05),which were decreased 0.707 and 0.337,respectively.The TT genotype in 960C > T locus could be used as the marker genotype for reproductive traits of three different lines of Large White pigs.

Key words: Large White pigs; BMPR-ⅠB gene; reproductive traits; correlation analysis

中图分类号:

S813.3

胡慧艳, 贾青, 侯胜奎, 刘津, 刘洋洋, 张伟峰, 赵坤峰, 王贵江. 大白猪BMPR-ⅠB基因多态性及其与繁殖性状的相关性研究[J]. 《中国畜牧兽医》, 2018, 45(4): 984-991.

HU Huiyan, JIA Qing, HOU Shengkui, LIU Jin, LIU Yangyang, ZHANG Weifeng, ZHAO Kunfeng, WANG Guijiang. Study on Polymorphism of BMPR-ⅠB Gene and Its Correlation with Reproductive Traits in Large White Pig[J]. , 2018, 45(4): 984-991.

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大白猪BMPR-ⅠB基因多态性及其与繁殖性状的相关性研究

Study on Polymorphism of BMPR-ⅠB Gene and Its Correlation with Reproductive Traits in Large White Pig

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