《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (4): 977-983.doi: 10.16431/j.cnki.1671-7236.2018.04.018

• 遗传繁育 • 上一篇    下一篇

PRR5L基因5'UTR多态性及其与脂肪性状、屠体性状的关联分析

王顺利1,2, 崔焕先2, 刘冉冉2, 李庆贺2, 郑麦青2, 文杰2, 赵桂苹2, 赵素梅1   

  1. 1. 云南农业大学云南省动物营养与饲料重点实验室, 昆明 650201;
    2. 中国农业科学院北京畜牧兽医研究所, 北京 100193
  • 收稿日期:2017-10-20 出版日期:2018-04-20 发布日期:2018-04-25
  • 通讯作者: 赵素梅(1974-),女,河南周口人,博士,教授,研究方向:动物分子营养与代谢调控,E-mail:zhaosm2009@126.com E-mail:zhaosm2009@126.com
  • 作者简介:王顺利(1991-),男,河南信阳人,硕士生,研究方向:动物分子营养与代谢调控,E-mail:WangSL_1992@163.com
  • 基金资助:

    国家自然科学基金项目(31060331、31260592、31760645);云南省教育厅重大专项(ZD2015007)

Polymorphisms of PRR5L Gene 5'UTR and Its Relationship with Fat and Carcass Traits in Chicken

WANG Shunli1,2, CUI Huanxian2, LIU Ranran2, LI Qinghe2, ZHENG Maiqing2, WEN Jie2, ZHAO Guiping2, ZHAO Sumei1   

  1. 1. Yunnan Key Laboratory of Agricultural Animal Nutrition and Feed Science, Yunnan Agricultural University, Kunming 650201, China;
    2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2017-10-20 Online:2018-04-20 Published:2018-04-25

摘要:

试验旨在研究类富脯氨酸5(proline rich 5 like,PRR5L)基因遗传变异对鸡脂肪性状及屠体性状的影响。以矮脚黄鸡为研究对象,根据GenBank公布的鸡PRR5L基因全序列(登录号:XM_015287209.1)设计扩增引物,利用PCR直接测序技术检测PRR5L基因的5'UTR区域单核苷酸多态性(SNPs),并用统计学方法分析其与鸡脂肪性状及屠体性状的关联性。结果发现,PRR5L基因5'UTR区域存在C480G、G514A和A579G 3个多态位点,均表现出3种基因型。χ2适合性检验结果发现,群体在G514A位点处于Hardy-Weinberg非平衡状态(P < 0.05);在C480G和A579G位点均处于Hardy-Weinberg平衡状态(P > 0.05)。基因型与性状关联分析结果显示,在脂肪性状方面,PRR5L基因3个多态位点各基因型间腹脂重、腹脂率和肌内脂肪含量差异均不显著(P > 0.05);在屠体性状方面,在G514A位点处AG与GG基因型的胸肌重差异显著(P < 0.05),其余指标差异均不显著(P > 0.05)。因此,可推断PRR5L基因5'UTR的G514A位点可能是一个影响鸡胸肌生长发育的位点,但该位点能否作为标识鸡屠体性状的分子标记辅助选择还需要进一步探究。

关键词: 鸡; PRR5L基因; 多态性; 脂肪性状; 屠体性状

Abstract:

This study was aimed to investigate the relationship between genetic variations of PRR5L gene with fat traits and carcass traits in chicken.Yellow Dwarf chicken were chosen as research material and primer was designed according to DNA sequence of chicken PRR5L gene (accession No.XM_015287209.1),the single nucleotide polymorphisms(SNP) of PRR5L gene 5'UTR region was detected by PCR direct sequencing.The results showed that there were three polymorphic sites of C480G,G514A and A579G,and all polymorphic sites showed three genotypes.The Chi-square test results showed that the C480G and A579G loci were in Hardy-Weinberg equilibrium (P > 0.05),the G514A locus was not in Hardy-Weinberg equilibrium (P < 0.05).In the fat traits,there were no significant differences among all genotypes of three polymorphic sites of PRR5L gene in abdominal fat weight,abdominal fat rate and intramuscular fat content (P > 0.05).In the carcass traits,there was significant difference between AG and GG genotypes in the G514A locus (P < 0.05).There was also no significant difference of all genotypes in the measured indexes at the C480G and A579G loci (P > 0.05).Therefore,the G514A locus of PRR5L gene 5'UTR might be a site that affected the growing development of chicken breast,however,we needed to further explored whether this site could be used as a marker for the identification of carcass traits in chicken.

Key words: chicken; PRR5L gene; polymorphisms; fat traits; carcass traits

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