《中国畜牧兽医》---唯一指定的官方网站 ›› 2016, Vol. 43 ›› Issue (7): 1843-1849.doi: 10.16431/j.cnki.1671-7236.2016.07.028

• 遗传繁育 • 上一篇    下一篇

宁夏西门塔尔杂种牛UCP2基因多态性及其与生长性状的关联性分析

蒋秋斐1,2, 罗晓瑜2,3, 洪龙2,3, 李欣1, 封元2,3, 巫亮2, 韩丽云1, 顾亚玲1   

  1. 1. 宁夏大学农学院, 银川 750021;
    2. 宁夏畜牧工作站, 银川 750001;
    3. 宁夏农林科学院, 银川 750001
  • 收稿日期:2016-01-04 出版日期:2016-07-20 发布日期:2016-07-22
  • 通讯作者: 顾亚玲 E-mail:guyaling@sina.com
  • 作者简介:蒋秋斐(1987-),女,宁夏银川人,博士生,研究方向:动物遗传育种与繁殖,E-mail:jiangqiufei@163.com
  • 基金资助:

    宁夏科技厅重大专项:优质高档肉牛新品系选育(2012ZDN0901)

Correlation Analysis between UCP2 Gene and Growth Traits in Ningxia Hibrid Simmental Cattle

JIANG Qiu-fei1,2, LUO Xiao-yu2,3, HONG Long2,3, LI Xin1, FENG Yuan2,3, WU Liang2, HAN Li-yun1, GU Ya-ling1   

  1. 1. School of Agriculture, Ningxia University, Yinchuan 750021, China;
    2. Animal Husbandry Extension Station, Yinchuan 750001, China;
    3. Faculty of Agricultural Sciences, Yinchuan 750001, China
  • Received:2016-01-04 Online:2016-07-20 Published:2016-07-22

摘要:

本研究利用PCR-SSCP技术结合DNA测序确定UCP2基因SNP位点,检测其在宁夏西门塔尔杂种牛群体中的多态性,并分析其与生长性状的关联性。结果表明,UCP2基因第1外显子存在3个SNPs位点,分别为T632C、A655G和A689C,结合PCR-SSCP结果分析后,分为AA、BB、AB 3种基因型。不同月龄体高、体重、胸围、体斜长,总体呈AA>AB>BB。AA基因型个体在不同月龄体高、胸围显著或极显著高于BB基因型(P < 0.05;P < 0.01),与AB基因型之间存在差异,但差异不显著(P > 0.05)。可以确定,T632C位点发生的碱基突变对宁夏西门塔尔杂种牛的体重性状选育存在显著意义,AA基因型个体可作为后期选育方向。

关键词: UCP2基因; 西门塔尔杂种牛; 多态性; 生长性状

Abstract:

To find genotype with fast growth rates in Ningxia hibrid Simmental cattle,the genetic variation of UCP2 gene were detected by PCR-SSCP method and DNA sequencing,then analyzed UCP2 gene polymorphism related with some growth traits.The results showed that there were three SNPs (T632C,A655G and A689C) in UCP2 gene exon 1,which divided into AA,BB and AB genotypes by PCR-SSCP method.Individuals with different genotypes in different months of age,body weight,body height, chest circumference and body length were AA > AB > BB.AA genotype in different age of body height and chest circumference were significantly different than BB genotype (P < 0.05;P < 0.01),and were no significant difference with AB genotype (P > 0.05).The results demonstrated that T632C locus had significant meaning for improving weight trait breeding of Ningxia hibrid Simmental cattle,AA genotype could be the breeding genotype.

Key words: UCP2 gene; hibrid Simmental cattle; polymorphism; growth traits

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