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Table of Content
20 February 2007, Volume 34 Issue 2
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动物营养与饲料科学
The Effects of Feeding Differently Processed Corn on the Digestion, Metabolism and Rumen Internal Environment in Yanbian Semi-fuzz Sheep
KANG Jindan;LI Deyun;ZHANG Jinji
2007, 34(2): 5-7.
Abstract
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1365
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After feeding differently processed corn as dietary to yanbian semi-fuzz sheep with fistulas, the pH varieties were not significantly different in all experimental groups. The concentration of NH
3
-N in the whole corn group was significantly lower than the other two groups after 3 h（P<0.05）, however, after 6 h, the mealy corn group was significantly lower than the other two groups（P<0.05）. The activities of CMCase and xylanase were not significantly different in all experimental groups. The variation of protozoa population of the granule corn group in rumen trended to be higher than the other two groups, but not significant. Also, there was no significant difference among all experimental groups in feeder nutriment digestibility.
Bovine Mammary Epithelial Cells beta-Casein Protein Detection and Karyotype Analysis
WANG Zhiguo;BU Dengpan;WANG Jiaqi
2007, 34(2): 8-11.
Abstract
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1454
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Using the Western-blotting technique to detect the beta-casein protein from culture medium of bovine mammary epithelial cells(BMEC) in vitro, which can identify the normal physiological function of BMEC;Karyotype analysis identify the number and figure of Chromosome of BMEC cultured in vitro. The results show: containing betacasein protein in culture medium of bovine mammary epithelial cells in vitro shows that the mammary epithelial cells with normal physiological condition; the number of chromosomes in normal, it is 60, and the karyotype agreement with the mammalian chromosome map, shows that the cell lines in vitro culture conditions not transformation.
添加剂与饲料
The Application of Cysteamine on the Growing Function of Silver Fox in Rearing Period
WANG Zhongyan;MA Jianzhang
2007, 34(2): 14-17.
Abstract
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1840
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To study effect of cysteamine on the growing function for silver fox in rearing period, 224 (112♂ +112♀) of two-month-old healthy silver foxes were selected, and the male weigh about 2.50 kg (P＞0.05),while the female around 2.10 kg (P＞0.05). Among them, 200 (100♂ +100♀) foxes were randomly selected for the test group, and the other 24 (12♂ +12♀) individuals were for the control group. Feeding experiments last about three months (from June 10 to August 31 in 2004). Experiment design of factor analysis, a multiple factor design, was adopted, in which two factors were repeated in experiments. Different amount of cysteamine (10 mg, 40 mg, 70 mg, 100 mg, 130 mg per kg of body weight BW) were added respectively at 5 different time intervals (1 d, 3 d, 5 d, 7 d and 9 d). There were 12 repeats of test in control groups, and a couple of individuals for each one. Finally, body weight gain, feed conversion ratio and feed intake were measured. Results showed that only the combination of 70 mg/kg BW of cysteamine added every 5days would produce the highest body weight gain and feed conversion ratio among total 25 combinations, meanwhile it increased by 15.08% (P<0.05) and 20.21%(P<0.01) respectively compared with those of the control group. However, feed intake of the test groups silver fox was lower than that of the control group, individuals of the combination of 70 mg/kg BW of cysteamine added every 5days decreased 4.52% (P<0.05) of feed intake compared with control group.
The Observation of Effect of Vegetable Silage
LI Yong;ZHU Pingjun;FAN Weijie;ZHAO Qingfeng;WANG Jingmei
2007, 34(2): 21-22.
Abstract
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1412
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The silage feed was made by mixtures of vegetable, corn meal, wheat bran and CH3COOH of percent 2 in this experiment. By observing, smelling and touching, the results showed with the increasing of time, the effects of silage feed were becoming more and more better. Furthermore, the effects of silage feed on the 30th day and 35th day were obviously better on 15th day. But there were no significant differences between the 30th day and 35th day.
Effects of Isovalerate on Rumen Fermentation in Simmental Steer
DUAN Yu;LIU Qiang;HUANG Yingxiang;WANG Cong;WANG Hao;GUO Gang
2007, 34(2): 23-25.
Abstract
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1199
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Four Simmental steers(average BW 420 kg, aged 2.5) with permanent rumen cannula and consuming a corn straw diet were used in a 4×4 Latin square design and supplemented with four levels(0, 0.02, 0.04 and 0.06g/kgW) of supplemental isovalerate to evaluate the effects of isovalerate on ruminal pH, NH3-N, and VFA. The results showed that ruminal pH of supplemental isovalerate 0.06 g/kgW decreased (P<0.05). The NH3-N concentrations of 0.04 g/kgW and 0.06 g/kgW group were lower than control and 0.02 g/kgW group significantly (P<0.05). Ruminal actate, butyrate, acetate/propionate, TVFA, and purine derivatives of urine were increased significantly (P<0.05) by isovalerate supplementation with 0.04 g/kgW. The supplementation of isovalerate had passive effect on rumen fermentation, the optimum dose was 0.04g/kgW.
奶业专栏
Drug Sentitive Test of the Main Pathogenic Germs Causing Holstein Subclinic Mastitis
SUN Qinghua;ZHAO Zongsheng;HE Gaoming
2007, 34(2): 29-30.
Abstract
(
1937
)
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Holstein subclinic mastitis has been investigated in dairy of Shihezi city in Xinjiang. Pathogenic bateria were isolated and identified from the milk of imported australian holstein surffered from subclinical mastitis,among them staphylococcus, occupy;streptococci, occupy;E.coli, occupy, merges to infect with of occupy, proceeded respectively the 15 strains medicine sensitivity experiment, which showed that pathogenic bacteria were resisitant to penicillin and streptomycin frequently used,while Quinolones had a fairly good inhibiting effection the pathogenic germs.
畜禽产品
The Evaluating Indexes of Beef Quality and Its Influencing Factors
YU Mei;MAO Huaming;HUANG Bizhi
2007, 34(2): 33-35.
Abstract
(
1952
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1.Key Laboratory of Animal Nutrition and Feed of Yunnan Agriculture University, Kunming 650201, China;
2.Yunnan Beef Cattle and Pasture Research Center, Kunming 650212, China
生理生化
Investigation on the Hematology Parameters of Chinese Laboratory Miniature Pig Breeds
YANG Shulin;REN Hongyan;WANG Heng;FENG Shutang;GAN Shixiang;WANG Aide;LI Kui
2007, 34(2): 38-41.
Abstract
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1388
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Eighteen hematology parameters of Wuzhishan miniature pig inbreeding line, Guizhou miniature pig breedand Guangxi Bama miniature pig closed colonies have been detected with the automatic hematology analytic instrument. The statistic results showed that there is only one parameter not significant in them, six ones were highly significant difference. The average coefficient of variance in all parameters of three breed were 0.243，0.19 and 0.182 in turn. Through out the comparison among three pig breeds and between them and the human reference value, Gttingen minipig, domestic pig and exotic Landrace pig, the important information were provided for the miniature pig utilized on the human medical study.
The Oxidative and Anti-oxidative Characteristics of Immune Tissuesin Chicken with Encephalomalacia
ZHANG Jinlong;XU Lili;LI Yanfei
2007, 34(2): 42-44.
Abstract
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1290
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The chicken was fed with low VE feedstuff to duplicate model of encephalomalacia.The oxidative and anti-oxidative characteristics of immune tissues in chicken with encephalomalacia were studied through detecting the content of NO,MDA and ROS in serum and immune tissues, the activity of XOD, GSH-Px and T-AOC. The results showed that the higher content of free radical and lipid peroxidation products,lower activity of GSH-Px and lower T-AOC in serum,spleen and bursa of chicken with encephalomalacia than that of control ones. The results indicated that oxidative stress occurred and the function of antioxidant system decreased in immune tissues of chicken with encephalomalacia.
Research Advance of Adiponectin and Adiponectin Receptor
DONG Biao;MENG He;GU Zhiliang;GONG Daoqing
2007, 34(2): 44-48.
Abstract
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1252
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Adiponectin is a cell factor secreted exclusively from adipocyte. It plays an important role in regulating the metabolism of glucose and lipid. This review summarizes research advance in the source, structure, regulation of glucose and lipid metabolism, expression and AdipoR of adiponectin.
Study on Effect of Egg Shape Index on Eggshell Strength, Egg Weight Loss, Allantoic and Amniotic Fluid Volume
YANG Haiming;GUO Chunyan;WAN Qiubei;WANG Zhiyue
2007, 34(2): 49-51.
Abstract
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1392
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In this experiment, egg shape index, egg weight loss in the incubation, allantoic and amniotic fluid volume were mensurated and analysed. The results showed that the egg shape index was correlative with the eggshell strengh, and the coefficient was 0.30. The eggshell strength was bigest when the egg shape index was from 0.800 to 0.819, more than and less than the range, the strength trended to descend. When the egg shape index was from 0.780 to 0.799, the egg weight loss was highest, but allantoic and amniotic fluid volume were smallest, more than and less than the range, the weight loss minished gradually, however allantoic and amniotic fluid volume increased little by little.
The Development of Diethylstilbestrol
HUANG Fen;YE Shaohui;GONG Zhenming
2007, 34(2): 51-54.
Abstract
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1790
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Diethylstilbestrol (DES), is a kind of nonsteroid synthesized estrogen. It was widely used in gynecological diseases and in the livestock industry as a growth promoter. Until mid70s, reports about neonatal DES exposed female had an increased risk to developing abnormal reproductive tracts and cancers were widely published. It was certified that DES was harmful to human and environment after over 30 years' study. But it is still controversial about whether DES should have been banned or not. This paper primarily discussed its application, side effects and the different views on DES.
遗传繁育
The Exon4 Polymorphism of IGF-Ⅰ Gene and Its Relationship with Partial Performances in Songliao Black Swine
XIAO Shuqi;ZHANG Jiabao;LI Shuang;ZHAO Zhihui;REN Wenzhi;ZHAO Zhongli;ZHANG Shumin;DAI Lisheng;GAO Yan;LI Yi
2007, 34(2): 55-57.
Abstract
(
1953
)
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IGF-Ⅰ gene was chosen as a candidate gene for growth and carcass traits of songliao black swine.The exon4(179bp) polymorphism of IGF-Ⅰ gene was detected by PCR-SSCP in 30 pigs,and the relationship between the polymorphism and growth and carcass traits was analyzed.The results showed that 2 alleles and 3 genotypes of IGF-Ⅰ gene were detected in songliao black swine,there was a higher degree of polymorphism; different IGF-Ⅰ genotypes significantly affected average daily gain after weaning.Average daily gain after weaning for AA genotype on IGF-Ⅰ was significantly higher than for AB and BB. The pigs with AA genotype had a lower lean percentage than those with AB genotype and BB genotype(P<0.01), and had a higher average backfat thickness than those with AB genotype and BB genotype(P<0.01). Other traits were not significantly affected.Therefore,the genetic analysis of IGF-Ⅰ gene revealed that IGF-Ⅰ locus significantly affected average daily gain after weaning, lean percentage,and average backfat thickness,it could be preliminarily deduced that IGF-Ⅰ gene was probably a major gene or a QTL linked gene which associated with growth and carcass traits.
Polymorphism Analysis on 5’-UTR and 3’-UTR of Duck Growth Hormone Gene
XU Shenghai;SHU Jingting;CHENG Jinhua;JU Huiping;HUANG Jun;CHEN Guohong
2007, 34(2): 58-60.
Abstract
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1435
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The single nucleotide polymorphism (SNP) of GH gene was investigated in various breeds, including beijing ducks, xihu mallards, cherry valley meat ducks, jinding ducks, shan partridge ducks, jingjiang ducks, shaoxing ducks, jinyun partridge ducks. Five primers for 5’-UTR and 3’-UTR of GH gene were designed according the database of duck genomic sequence and the SNPs were detected by PCR-SSCP method. Polymorphism (C/G at 230 and C/A at 244 ) was only found among individuals within jinding ducks, jingjiang ducks, shaoxing ducks. Population genetics analysis showed that the frequency of AB genotype in jinding ducks was significantly higher than that of jingjiang ducks and shaoxing ducks and the frequency of A allele in jinding ducks was significantly higher than that in jingjiang ducks and shaoxing ducks.
Blood Protein Polymorphisms and Classification in Five Chinese Native Goat Breeds
DENG Zaofu;OUYANG Xuxiang;LIU Hexiang;HUANG Shengqiang;YIN Xiaoping;TAN Shengguo;HU Shuguang
2007, 34(2): 61-64.
Abstract
(
1922
)
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In order to exploit and utilize goat resource, 8 blood protein polymorphismsin had been studied by applied polyacrylamide gel electrophoresis for 5 Chinese native goat breeds．The genotypic and allele frequencies of 5 polymorphic loci (Hb, Am,Tf,Po, Alb) in each breed were calculated. The overall genetic distances were calculated by allele frequencies. Dendrogram was drawn up by group-avarage cluster analysis. The Chinese native goat breeds could be classified as 3 group:Xiangdong Black and Wuxue, Inner-Mongolian White and Gaixian White, Matou. In addition, dying methods were improved for serum proteins.
DNA Molecular Markers Clarified the Taxonomic Status of Mites from Mammals and Humans
GU Xiaobin;YANG Guangyou;FANG Chunlin;HAO Guiying;WU Kaibo
2007, 34(2): 64-66.
Abstract
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1703
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Mites caused of mange in mammals and humans and of scabies in humans have a very host range. The question as to whether these mites might be divided into different species or whether they are monospecific, has been the subject of an ongoing debate. A generally accepted answer has not yet been gound. DNA molecular markers were generally used to clarify the taxonomic status.
Mouse Oocyte Enucleation with Modified Surface Tension Assisted Method
LI Jingchun;FANG Nanzhu
2007, 34(2): 67-68.
Abstract
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1337
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To study the reliability of the new enucleation method for mouse oocyte：modified surface tension assisted method. Modified surface tension assisted method is significantly better than STA’s method in the enucleated rates of time(P<0.05). Rate of Enucleation is similar between two groups. Modified surface tension assisted method is a efficient and simple enucleation method with the advantage of saving manipulating time and nondamage to mouse oocyte．
Progress of Inhibin Immunization in Animal Reproduction
PENG Zhilan;CHU Mingxing;CHEN Hongquan
2007, 34(2): 69-73.
Abstract
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1559
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Inhibins are dimeric gonadal glycoprotein hormones that negatively regulate pituitary FSH synthesis and secretion belonging to the transforming growth factorβsuperfamily of growth factors. Inhibin immunization has an important potential use in advancing the animal reproductive performance. In this paper, we briefly introduced the structure, characteristic of inhibin, as well as the mechanisms, the types, the methods and the influencing factors of inhibin immunization and its application in livestock husbandry.
Establishment of Real- time PCR for β-actin Gene of Chicken
MA Li;XIE Xiulan;YUE Hua
2007, 34(2): 73-75.
Abstract
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1810
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According to the chicken β-actin gene sequences available in GenBank, a pair of primers was designed for establish a SYBR Green I quantitative real-time PCR method for β-actin gene of chicken. To establish the standard curve, the product of conventional PCR served as a standard. The analysis of melting curve was also carried out. The results show the linear range of Ct value was from 12 to 31 with a good correlation coefficient(r=0.996). The melting curve show a single peak with a 88±0℃ Tm value. The real-time PCR assay developed in this study can detect β-actin in expand range with high efficiency and less time. The assay provide the basis for β-actin gene of chicken as a reference gene in quantitative analysis of mRNA expression.
The Application of RNA Interference
WANG Jun;ZHANG Yifang;ZHANG Ye
2007, 34(2): 76-78.
Abstract
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1244
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Putting doublestranded RNA into cell could cause homologous genes silencing.This phenomenon was called RNA interference. In this paper, the history，mechanism of action， application and perspective of RNA interference were summarized. Especially summarized from the application of RNA interference.
The Application of Bioinformatics on Livestock and Poultry Genomics Study
LI Caie;;MA Yuehui;YE Shaohui
2007, 34(2): 79-82.
Abstract
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1398
)
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Bioinformatics is an importand component of the whole life science,has become the advanced research domain of life science.This paper mainly discuss from the following aspects:livestock and poultry genomics,livestock and poultry bioinformatics and its application on livestock and poultry genome study and also propect ist foreground.
DNA Methylation, Histone Modifications and Genes Silencing
SUO Xiaojun;ZHANG Nian
2007, 34(2): 82-85.
Abstract
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1393
)
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DNA methylation, histone deacetylation, and methylation of histone H3 at lysine 9 are the three bestcharacterized covalent modifications associated with a repressed chromatin state, and they are all involved in genes silencing. Recent advances highlight an intricate connection of interactions among these processes, generating a selfreinforcing epigenetic cycle that might perpetuate a repressed chromatin state. Histone deacetylation and methylation at lysine 9 of H3 might also contribute to the establishment of DNA methylation patterns. What's more, the recent studies show that CpG methylation and other histone modifications are correlated.
疾病防治
Development of a Cold-SSCP Technique for the Identification of Fasciola Spp
DENG Guoxiang;ZHANG Hanyun;CHEN Beirong;LI Qiuyan;LIN Ruiqing;MA Nini;ZHU Xingquan
2007, 34(2): 86-88.
Abstract
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1266
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A nonisotopic single strand conformation polymorphism (ColdSSCP) technique was established to differentiate fasciola hepatica from fasciola gigantica from different geographical locations in China using the first internal transcribed spacers (ITS1) of ribosomal DNA (rDNA) as genetic marker. The partial ITS1 was amplified by PCR from individual fasciola and the amplicons were subjected to nondenaturing gel electrophoresis. ColdSSCP analyses showed that fhepatica and fgigantica displayed distinct SSCP profiles, allowing their equivocal differentiation from each other. Sequencing analyses supported the results of ColdSSCP. The establishment of the ColdSSCP approach for the identification and differentiation of
fhepatica and fgigantica has important implications for further studies of fasciola spp.
Identification of Antibioticresistant Phenotype and Genotype in Methicillinresistant Staphylococcus
WANG Jiaoyu;HAN Wenyu;LEI Liancheng;FENG Xin
2007, 34(2): 89-92.
Abstract
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1369
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We identified 16 isolates of methicillinresistance staphylococcus aureus and 4 isolates of menthicillinresistance coagulasenegative staphylococcus by drugresistant phenotype and genotype assay to 41 staphylococcal isolates.The rate of menthicillinresistance staphylococcus is 48.8%.It not only suggested that veterinary clinical must boost the reconstruction to MRS,but also provide the reference of clinical appraisal to MRS.
The Immunological Enhancement and Application of Propolis
WANG Ru;XIE Yinqian;SHEN Zhiqiang;MIAO Xiaoqing
2007, 34(2): 93-95.
Abstract
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1441
)
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Propolis extremely complex chemical composition determines its broad biological actions. Propolis have anti-bacterial, anti-virus and immunological enhancement etal: particles and antigens link with each other into composites (ISCOM) structure with antigen “warehouse” and the role of immune stimulating compounds: although propolis itself has no antigen property, it plays a part as an immune adjuvant in the promotion of antibody generation.the role and synergies between themselves decide propolis is a immunopotentiator. Since the 1960s, its immunological enhancement and its application in vaccine adjuvant are futher researched, and have achieved satisfactory results, which provide some theoretical guidance to the research and development of new vaccines.
Detection of Salmonella in Chicken with Fluorescence Polymerase Chain Reaction
JIA Guangle;LIN Xiangmei;HAN Xueqing;WU Shaoqiang;LIU Jian;MEI Lin
2007, 34(2): 96-97.
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1309
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In present article, there are two positive standard salmonella bacteria, two positive sample about chicken and a sample of aseptic water. This samples are detected with fluorescence polymerase chain reaction. The results showed: The method is efficient and accurate. Furthermore, it took only 30 hours for whole PCR process. It is concluded that PCR is a specific, sensitive, rapid, simple and convenient method for detection of salmonella. It may have great values for the entryexit inspection and quarantine.
Studies of Pathologyical Changes of Lung and Extrapulmonary Organs in M ovipneumoniae Infection of Sheep
TAO Yue;LI Xinping;LIN Weimin;ZHANG Xiaoen;SUN Yue;TIAN Xinling
2007, 34(2): 98-99.
Abstract
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1340
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M ovipneumoniae is pathogen to cause Contagious ovine pleuropneumonia. Generally M ovipneumoniae may cause sheep's peripneumonia. Besides lung, M ovipneumoniae may be detected in other internal organs with culture and PCR detection, the probability to separate M ovipneumoniae is higher form extralung organ of the serious illnesss sheep. Besides M ovipneumoniae can mainly cause the lung pathological change,it can cause lesion of heart, liver, spleen, kidney, brain, lymph node as well. Lung is not only one critical organ by M ovipneumoniae infection.
The Cellular Immune Function of Chicken with Nutritional Encephalomalacia
WANG Ben;ZHANG Hongling;LI Yanfei
2007, 34(2): 100-101.
Abstract
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1281
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The number of the white blood cell(WBC),the sum total of lymphocytes ,the activity of T lymphocytes ANAE+ and the concentration of interleukins were studied by Acid αnaphthyl acetate esterase，（ANAE） dye method, and radio immunoassay(RIA) in the chicken with encephalomalicia induced by giving the feed being deficient in VE and extra fish oil artificially . The results showed that the number WBC,the sum total of lymphocytes and,activity of T lymphocytes ANAE+ and the concentration of IL1,IL2 in the ataxic chicks were significantly lower than that of the control group. The results indicated that vitamin E deficiency has evidently inhibited in the cellmediated immune functions of the chicken.
A Cross Immunoprophylaxis Experiment with Epidemic Avian NDV Strain and Avian NDV Strain
LIU Yan;XUN Laiwu;YANG Xiaohua;HE Huachuan
2007, 34(2): 102-104.
Abstract
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1314
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12 day old chicken, which averange titres of NDV antibodies is 2log2, was immunized with the oil adjuvant NDV velogenic strains vaccine and the oil adjuvant NDV La Sota strains vaccine. After the inoculability, the serum was pooled every week, and the titres of NDV antibodies was showed by the MicroHIA. The peak titres of NDV antibodies was appeared the 21th day, and the 6log2 high titres was keeping above 30 days. 40 days later in the immunization, the chicken were challenged by NDV velogenic isolates and NDVF48E9 isolates, and no chicken was infected. The percentage of protection from the two vaccines are 100%. The test of challege indicated that immunigenicity difference between the oil adjuvant NDV velogenic strains vaccine and the oil adjuvant NDV LaSda strains vaccine is not remarkable.
Serodiagnosis of
Neospora Caninum
Infection in Improvement Cashmere Goat by Enzyme-Linked Immunosorbent Assay with Recombinant Truncated NcSAG1t
LU Yan;WANG Geping;MA Liqing
2007, 34(2): 109-111.
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1774
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N caninum
；NcSAG1t；ELISA；改良绒山羊；抗体
The Control of PR Disease on Intensive Pig Farms in China
2007, 34(2): 112-115.
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1580
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PR is an acute infectious disease caused by PR virus. Vaccines immune is a key way to protection against PR, and we should adopt actual control measures in according to the severity degree to which it’s infected by wild virus in intensive pig farms. There are different characters and the usage scopes of the normal vaccines for inactivated vaccine, weak poison vaccine and gene deficiency vaccine, and after a series of analysis we find out that using gene deficiency vaccine is the best choice to protection against PR at pig farms. We finally pointed out the importance of immunology monitoring after we had analyzed those Disputed issues on immune programs and methods such as the 1st immune day, no time immune day, the interval time of immune as well as immune through dropping noses. At last we put forward the measures and feasibility to perish PR disease on intensive pig farms and summarize successful experiences which are from home and abroad. We hope it can offer some great practical directions on control PR disease.
Research on the Diagnosis and Treatment of H.Canis
DONG Xia;LIU Fenghui
2007, 34(2): 115-116.
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1202
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In the research ,direct microscopic exam, hematology inspection, idodine brake test, vigor experiment were used to diagnose 20 dogs admitted by animal hospital. As a result, 18 of them were determined dominant or latent H.Canis . Refering to the above diagnosis experiment, 10 dogs were sampled out at random among the 18 infectious dogs and were divided into 5 groups to carry on in vivo pharmacokenetics experiment. The dogs were injected Berenil+Imidocarb , Berenil, Imidocarb and Special effect Mi Xian respectively in different groups; the last group is a control group, The results indicated that Berenil combining with Imidocarb shows the best effects; Berenil and Imidocarb used separately were secondly effective; Special effect Mi Xian respectively hardly showed any effects.
The Prevention & Control of Infectious Laryngotracheitis of Chickens
QIAN Fengqin;GUO Ning
2007, 34(2): 117-119.
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1286
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This essay summarized the pathogeny of infectious laryngotracheitis of chickens,the character of its cultivation, pathogenesis,the latent infection,the method of diagnosis and its prevention and control,meanwhile ,the essay prospected how to control and deracinate infectious laryngotracheitis of chickens.
The Separation Appraisal and Drugs Susceptibility Test of Chicken’s
Escherichia Coli
Disease Etiology
ZOU Ling;TANG Dong;LIU Wenhua;REN Huiying
2007, 34(2): 119-121.
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1674
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Fourty-four strains of
escherichia coli
from chickens were isolated from cases that were suspected to be
escherichia coli
, and the pathogen's characteristics were identified. It was found that the biochemical characters of these bacteria were fundamentally identical and agreed with documents. Moreover, the serotypes of 39 isolates were identified. Thirteen types of serum O were found out, among which O
78
,O
35
,O
24
,O
1
,O
15
,O
76
and O
88
were main serotypes with a 84.6% of all the isolates. The dominant serotype of these isolates was O
78
with a 38.5% of all the isolates. Antibiotic resistance of 44 strains
escherichia coli
to nine antimicrobial drugs (Cefazolin,Gentamicin,Amikacin,streptomycin,Ciprofloxacin,Norfloxacin,Penicillin,Tobramycin,Erythromycin) was detected and analyzed by disk diffusion susceptibility test. The results indicated that the isolates showed a greater or lesser degree resistance to nine antimicrobial drugs. Most isolates showed resistance to multiple antibiotics and displayed a highest level resistance to Penicillin and Erythromycin with a frequency of 97.7%,followed by Ciprofloxacin with a frequency of resistance of 81.8%.While most isolates displayed a higher level susceptibility to Amikacin, Tobramycin and Gentamicin,especially to Amikacin with a frequency of 90.9%.
How to Differential Diagnosis the Symptom-group about Alopecia of Dogs
WEI Dong;ZHANG Naisheng
2007, 34(2): 122-125.
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1815
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犬；脱毛；症状；鉴别诊断
动物生产
Study on the Effect of Superovulation in Qinchuan Cattle
LI Jinhui;WANG Xinzhuang;LIANG Jiakui;NIU Hui;WANG Yimin;WANG Yerong;WANG Mu.;GAO Shunqi;WANG Tao
2007, 34(2): 136-138.
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1676
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31 Qinchuan cattle were superovulated, 20 cattle were effective with the rate 64.5%, and 6.45 embryos were obtained on average. Among them, 4.20 embryos were useful. The embryos obtained from each cattle and useful embryos of the 300 mg group were significantly higher than groups that treated with FSH 260 mg and 340 mg per cattle (P<0.05). The obtained (8.56) and useful embryos (6.00) per cattle treated in spring and summer were significantly higher than that treated in autumn and winter (4.73, 2.73) (P<0.05). 10 cattle were superovulated repeatedly with the interval 70 days. There were not any significantly different between each superovulation (P<0.05).
Study on Milking Performance of Murrah × Local Buffalo (F
1
)
ZHANG Jicai;WANG Ankui;FU Meifen;WANG Zhe;YANG Guorong
2007, 34(2): 145-147.
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1681
)
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This paper were introduced the study on milk performance of Murrah×Local Buffalo (F
1
) at Xiaoshao, Kunming. The results showed that the lactation was longer, average was 292±45.5 d, fewer was 370 d.The average milk yield was 2015.38 kg (1761.25~2299.54 kg), daily milk yield was 6.39 kg.
Trends of Drugresistance and Pathogens Isolated from Animals in Zhejiang Province
NI Baifeng;ZHU Jiaxin;LU Guolin;YU Guoqiao;XU Hui;ZHAO Lingyan;WANG Yong
2007, 34(2): 147-149.
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1649
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Bacteria culture isolation and antibiotic sensitivity tests from 516 animals in Zhejiang province had been performed from 2002 to 2005. The main pathogens isolated were staphylococci, streptococci and E.coli, being 18.92% of total isolated bacteria in 2002~2003, 24% in 2003~2004 and 16.21% in 2004~2005. Among grampositive coccobacteria, staphylococci occupied 6.90% in 2002 and increased to 23.25% in 2005. Drugresistance of these three main pathogens to sulfamethoxazole ,tetracycline, kanamycin, streptomycin were 88.78%, 100%, 83.33% and 82.22%. Drug-resistance of staphylococci and E.coli to enoxacin was 11.31% and 22.62%, respectively, suggesting enoxacin had efficiently Bactericidal effect on staphylococci and E.coli. Similarly, ampicillin showed bactericidal effect on streptococci and only 34.32% of streptococci isolated in 2002~2005 showed ampicillinresistance. These results suggest that drugresistance of pathogens isolated from animals increase gradually.
经验交流
The Potting Experiment of Composting Effect
WANG Qian;CHENG Jiemin;WANG Zhifeng
2007, 34(2): 161-162.
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1879
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Using the different composting products carried on the rape potting experiment, the result indicated that the chemical fertilizer production increase scope was the biggest, the rape fresh weight increased 76.4%. The organic fat VIII of rape vitamin C content enhanced the scope to be the biggest, is 22.2%; The organic fat III of rape soluble sugar content increases the scope to be the biggest, is 181%.
Discuss on Veterinary Technological Progress & Public Health Security
SONG Dongliang;HE Yuying
2007, 34(2): 163-166.
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1670
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This article, initiated the veterinary science basic concepts, is summarized the Progressive status in veterinary technology under the medicine science and technology influence and analyzed relationships between the veterinary technology and the public health which developing along the medicine advance in technology. It is suggested that the veterinary technical progresses existence superiority and the hidden danger to the public health entire peace. And constructing the harmonious relationship between the veterinarian technical progresses and the public health has carried on the forecast.
