›› 2007, Vol. 34 ›› Issue (2): 86-88.

• 疾病防治 • Previous Articles     Next Articles

Development of a Cold-SSCP Technique for the Identification of Fasciola Spp

DENG Guoxiang1，ZHANG Hanyun2,CHEN Beirong1，LI Qiuyan1，LIN Ruiqing1, MA Nini1， ZHU Xingquan1
   

  1. 1.College of Veterinary Medicine, South China Agricultural University，Guangzhou 510642，China; 2.Shiqiao Animal Husbandry and Veterinary Station, Panyu District, Guangzhou 511400, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-20 Published:2007-02-20

Abstract: A nonisotopic single strand conformation polymorphism (ColdSSCP) technique was established to differentiate fasciola hepatica from fasciola gigantica from different geographical locations in China using the first internal transcribed spacers (ITS1) of ribosomal DNA (rDNA) as genetic marker. The partial ITS1 was amplified by PCR from individual fasciola and the amplicons were subjected to nondenaturing gel electrophoresis. ColdSSCP analyses showed that fhepatica and fgigantica displayed distinct SSCP profiles, allowing their equivocal differentiation from each other. Sequencing analyses supported the results of ColdSSCP. The establishment of the ColdSSCP approach for the identification and differentiation of
fhepatica and fgigantica has important implications for further studies of fasciola spp. 

Key words: fasciola; rDNA; ITS-1; Cold-SSCP

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