一株弗氏柠檬酸杆菌的分离鉴定及致病性研究

doi:10.16431/j.cnki.1671-7236.2018.02.024

Abstract

Abstract:

In order to determine the cause of disease in tilapia, the dying fish was carried out in sterile, a strain of bacterium (GXKJDX111) was isolated from the heart, through morphological observation, physiological and biochemical identification and 16S rDNA analysis, we identified the isolate as Citrobacter freundii, the results of drug sensitivity test showed that the isolate had very strong resistance, among the 20 kinds of drugs tested, the isolate was resistant to 13 kinds of drugs, including enrofloxacin, penicillin and so on, and had the sensitivity to 5 kinds of medicines, including ceftriaxone, ceftazidime, cefadroxil, piperacillin, Shubactam etc. Artificial infection showed that the bacterium had strong pathogenicity to grass carp, Pelteobagrus fulvidraco, tilapia and Procyprismerus. The result clarified the causes of the prevalence of tilapia, and could guide the farmers to use drugs reasonablely in the breeding process.

Key words: tilapia; Citrobacter freundii; 16S rDNA; drug resistance

CLC Number:

S943

SHI Zhengyu, YI Yi, HUANG Desheng, LUO Fuguang, HUANG Jie, LU Jingdi, LI Ya. Isolation, Identification and Pathogenicity of a Strain of Citrobacter freundii[J]. , 2018, 45(2): 479-485.

References

[1] BOWEN S H. Digestion and assimilation of periphytic detrital aggregate by tilapia mossambica[J]. Transactions of the American Fisheries Society, 1981, 110(2):239-245.
[2] 金霄,李灿,孙宗毅,等. 罗非鱼常见细菌病及防治方法[J]. 渔业致富指南,2016,22:47-50.JIN X,LI C,SUN Z Y, et al. Common bacterial diseases and prevention methods of tilapia[J]. Fishery Guide to be Rich, 2016,22:47-50. (in Chinese)
[3] BOWEN P R, WOOSTER G A, GETCHELL R G, et al. Streptococcus iniae infection of tilapia Oreochromis niloticus in a recirculation production facility[J]. Journal of the World Aquaculture Society, 1998, 29(3):335-339.
[4] 杨宁,黄海,张希,等.尼罗罗非鱼嗜水气单胞菌病的病原分离鉴定和药敏试验[J].水产科学, 2014,33(5):306-310.YANG N,HUANG H,ZHANG X. Isolation,identification and drug sensitive test of pathogen Aeromonas hydrophila from Nile tilapia Oreochromis nilotica[J]. Fisheries Science, 2014, 33(5):306-310. (in Chinese)
[5] 邓显文,谢芝勋,刘加波,等.罗非鱼荧光假单胞菌的分离鉴定[J]. 广西农业科学, 2010,41(6):612-615.DENG X W,XIE Z X,LIU J B, et al. Isolation and identification of Pseudomonas fluorescens in tilapia[J]. Guangxi Agricultural Sciences, 2010, 41(6):612-615. (in Chinese)
[6] ELDAR A, BEJERANO Y, BERCOVIER H. Streptococcus shiloi and Streptococcus difficile:Two new streptococcal species causing a meningoencephalitis in fish[J]. Current Microbiology, 1994, 28(3):139-143.
[7] 王利,魏勇.鲫鱼弗氏柠檬酸杆菌的鉴定及系统发育分析[J].水产科学,2012,8:481-484.WANG L,WEI Y. Identification and phylogenetic analysis of bacterium Citrobacter freundii from crucian carp Carassius auratus by 16S rDNA genes[J]. Fisheries Science, 2012, 8:481-484. (in Chinese)
[8] 王利,魏勇,龙钟明,等.鲫鱼弗氏柠檬酸杆菌的耐药表型及耐药基因检测[J].黑龙江畜牧兽医,2012,15:14-16.WANG L,WEI Y,LONG Z M, et al. Detection of the drug-resistant phenotypes and genes in Citrobacter freundii isolated from Carassius aumtus[J]. Heilongjiang Animal Science and Veterinary Medicine, 2012, 15:14-16. (in Chinese)
[9] 陈翠珍,房海,葛慕湘,等. 锦鲤弗氏柠檬酸杆菌的鉴定[J]. 河北科技师范学院学报,2011, 25(4):21-25.CHEN C Z,FANG H,GE M X,et al. Identification of Citrobacter freundii from ornamental carp (Cyprinus carpio L.)[J]. Journal of Hebei Normal University of Science and Technology, 2011, 25(4):21-25. (in Chinese)
[10] 吕爱军,胡秀彩,朱静榕,等. 弗氏柠檬酸杆菌感染诱导斑马鱼皮肤免疫相关基因的差异表达[J]. 水产学报, 2012,36(3):359-366.LV A J,HU X C,ZHU J R, et al. Differential expression of immune-related genes from skin of zebrafish induced of Citrobacter freundii[J]. Journal of Fisheries of China, 2012, 36(3):359-366. (in Chinese)
[11] 蔡妙英, 东秀珠. 常见细菌系统鉴定手册[M]. 北京:科学出版社,2001.CAI M Y,DONG X Z. Common Bacterial System Identification Manual[M].Beijing:Science Press, 2001.(in Chinese)
[12] FERARO M J. Performance standards for antimicrobial susceptibility testing[S]. National Committee for Clinical Laboratory Standards, 2001.
[13] BARNES L A, CHERYWB A.A group of paracolon organism having apparent pathogenicity[J].American Journal of Public Health and the Nation's Health,1946,36:481-483.
[14] 薛巧,赵战勤,刘会胜,等. 弗氏柠檬酸杆菌对动物和人致病性研究进展[J]. 动物医学进展, 2015,36(7):81-85.XUE Q,ZHAO Z Q,LIU H S, et al. Progress on Citrobacter freundii pathogenicity in animals and humans[J]. Progress in Veterinary Medicine,2015,36(7):81-87. (in Chinese)
[15] 周加利. 柠檬酸杆菌O抗原基因簇RFLP分型及5个O血清型基因簇的破译[D].天津:南开大学, 2011.ZHOU J L. The RFLP typing of citrate bacillus O antigen gene cluster and the deciphering of 5 O serotype gene clusters[D].Tianjin:Nankai University,2011. (in Chinese)
[16] 孙洋,郭学军,周博,等. 东北虎出血性腹泻病原分离与鉴定[J]. 中国兽医杂志,2008,44(9):89-90.SUN Y,GUO X J,ZHOU B,et al. Isolation and identification of hemorrhagic diarrhea in the Amur tiger[J]. Chinese Journal of Veterinary Medicine,2008, 44(9):89-90. (in Chinese)
[17] 陈红莲,宋光同,何吉祥,等. 克氏原螯虾弗氏柠檬酸杆菌的分离鉴定与药敏试验[J].淡水渔业,2014,1:73-77.CHEN H L,SONG G T,HE J X, et al. Isolation,identification and antibiotics susceptibility test of Citrobacter freundii from Procambarus clarkia[J]. Freshwater Fisheries, 2014, 1:73-77. (in Chinese)
[18] 沈锦玉,顾志敏,潘晓艺,等.红螯螯虾弗氏柠檬酸杆菌病病原的分离与鉴定[J].中国水产科学,2005, 12(2):197-200.SHEN J H,GU Z M,PAN X Y, et al. Isolation and identification of Citrobacter freundii from Cherax quadricarinatus[J].Journal of Fishery Sciences of China,2005, 12(2):197-200. (in Chinese)
[19] 李华,邢殿楼,白国福,等. 弗氏柠檬酸杆菌对河蟹致病性的研究[J]. 水生生物学报,2001,25(3):217-223.LI H,XING D L,BAI G F, et al. Studies on disease of mitten crab caused by Citrobacter freudii[J]. Acta Hydrobiologica Sinica, 2001, 25(3):217-223. (in Chinese)
[20] 杨方园,关瑞章,李忠琴,等. 花鳗鲡病原菌弗氏柠檬酸杆菌的鉴定[J]. 集美大学学报(自然科学版),2013,18(2):81-87.YANG F Y,GUAN R Z,LI Z Q, et al. Identification of pathogenic Citrobacter freundii isolated from Anguilla marmorata[J]. Journal of Jimei University (Natural Science), 2013, 18(2):81-87. (in Chinese)
[21] 韦阳道,易弋,黎娅,等.胡子鲶致病性摩氏摩根菌的分离鉴定与致病性研究[J].中国畜牧兽医,2017,44(1):221-226.WEI Y D,YI Y,LI Y, et al. Isolation and identification of Morganella morganii in Clarias fuscus and analysis of its pathogenicity[J]. China Animal Husbandry & Veterinary Medicine,2017,44(1):221-226. (in Chinese)
[22] 胡秀彩,吕爱军,刘云燕,等. 罗非鱼肠道中弗氏柠檬酸杆菌的分离与鉴定[J]. 黑龙江畜牧兽医, 2014,21:174-176.HU X C,LV A J,LIU Y Y, et al. Isolation and identification of Citrobacter freundii from the intestine of tilapia[J]. Heilongjiang Animal Science and Veterinary Medicine, 2014, 21:174-176. (in Chinese)

Isolation, Identification and Pathogenicity of a Strain of Citrobacter freundii

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