牛源荚膜A型多杀性巴氏杆菌疫苗候选株的筛选及鉴定

doi:10.16431/j.cnki.1671-7236.2018.02.023

Abstract

Abstract:

In order to screen the inactivated strain of bovine capsular type A Pasteurella multocida, which was fast, virulent, immunogenicity and small side reaction, six strains of bovine capsular type A Pasteurella multocida were collected in different areas. The growth curve of the medium, the virulence of mice, the content of lipopolysaccharide (LPS) and the antibody titer in mice and rabbits immunized with the inactivated bacteria of each strain were determined, and the attack protection test was carried out. The results showed that the growth rate of Pm2, Pm3 and Pm5 isolates were faster, the virulence were stronger and the LPS content were higher. All of them contained ptfA and fimA genes related to virulence and immunity. No significant adverse reactions were found in immunized mice and rabbits, the serum antibody was 1:64 to 1:128 after 14 days of the second immunization, and all animals tested survived after virulent challenge, that in the PBS control group were all died. The results indicated that Pm2, Pm3 and Pm5 could be used as Pm inactivated vaccine candidate strains, and of which Pm3 was the preferred strain.

Key words: bovine Pasteurella multocida; capsular serum type A; inactivated vaccine; immuno-protection; antibody titer

CLC Number:

S858.23

ZHU Ling, LIU Xuwei, YAN Wenliang, ZHANG Rui, HAN Xiaoli, YAN Genqiang. Screening and Identification of Bovine Capsular Type A Pasteurella multocida Vaccine Candidate Strains[J]. , 2018, 45(2): 471-478.

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Screening and Identification of Bovine Capsular Type A Pasteurella multocida Vaccine Candidate Strains

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