核桃青皮水提物对肉鸡肠道微生物菌群结构的影响

doi:10.16431/j.cnki.1671-7236.2020.02.034

Abstract

Abstract: The experiment was aimed to study the abundance and diversity of the intestinal microflora of broiler chickens by feeding water extract from walnut green husks,and to explore the effect of water extract from walnut green husks on the intestinal microflora community structure of broiler.A total of 240 local chickens of 1 day old were randomly divided into 2 groups,the control group and test group,with 4 replicates in each group and 30 chickens in one replicate.The experimental group began to feed the water extract of walnut green husks at 28 days of age.After feeding for 6 weeks,10 fecal samples were collected from each replicate,and the changes in number and structure of intestinal flora were compared by 16S rDNA V3-V4 region high-throughput sequencing.The high-throughput sequencing results showed that all samples contained a total of 17 phyla and 48 genera;The results of diversity index analysis showed that the intestinal flora richness on broiler chicken fed water extracts were increased.From the level of the phylum,the abundance of Firmcutes bacteria increased by 2.208% (P > 0.05),and the abundance of Proteobacteria decreased by 2.218% (P > 0.05).On the genus level,the abundance of Escherichia coli was reduced by 5.35% (P < 0.05).The abundance of the Enterococcus was increased by 9.63% (P < 0.05).The results showed that the abundance and diversity of intestinal flora of broilers increased significantly after feeding the water extract of walnut green husks (P < 0.05).In conclusion,the water extract of walnut green husks could effectively regulate the intestinal flora of broilers and had beneficial effect on intestinal health.

Key words: walnut green husks; broiler; intestinal microbes; 16S rDNA; diversity

CLC Number:

S853.7

ZENG Weihuan, LIU Yingbo, LI Rong, XIAO Wangcheng, DUAN Gang, QIN Li, DAI Feiyan. A Effect of Walnut Green Husks Extract on Intestinal Microorganisms in Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 612-619.

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A Effect of Walnut Green Husks Extract on Intestinal Microorganisms in Broilers

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