黄颡鱼迟钝爱德华菌的分离鉴定及其致病性研究

doi:10.16431/j.cnki.1671-7236.2018.03.031

Abstract

Abstract:

For determining aetiological agent of Pelteobagrus fulvidraco,the pathogen was isolated from the diseased Pelteobagrus fulvidraco and named as GDYM20160809.The isolate was identified by morphological observation,physiological and biochemical characteristics,16S rDNA analysis and phylogenetic tree construction.The susceptibility test of the isolate was carried out by filter paper diffusion method.The results showed that the isolate was gram-negative bacillus and the 16S rDNA fragment was amplified by PCR.The 16S rDNA fragment was sequenced and compared by BLAST,the isolate was 100% homologous to Edwardsiella tarda.Combined with the results of physiological and biochemical identification,the isolate was determined as Edwardsiella tarda.Susceptibility test results showed that the isolate was sensitive to sulfonamides,cephalosporins,penicillins and cotrimoxazole and so on,and was resistant to sodium sulfasalazine,novobiocin,polymyxin B,amikacin,kanamycin and vancomycin six kinds of drugs.The artificial infection test results confirmed that the isolate strain had strong pathogenicity to tilapia,Procypris merus,grass carp and Pelteobagrus fulvidraco.The study provided a theoretical basis for the effective prevention and control of catfish bacterial diseases,and could guide farmers to rational drug use.

Key words: Pelteobagrus fulvidraco; isolation and identification; 16S rDNA; Edwardsiella tarda

CLC Number:

S852.61

SHI Zhengyu, YI Yi, HAN Shuyu, LUO Fuguang, HUANG Jie, LU Jingdi, LI Ya. Isolation and Identification of Edwardsiella tarda in Pelteobagrus fulvidraco and Analysis of Its Pathogenicity[J]. , 2018, 45(3): 807-813.

References

[1] 成庆泰.中国鱼类系统检索[M].北京:科学出版社,1987. CHENG Q T.Systematic Synopsis of China Fishes[M].Beijing:Science Press,1987.(in Chinese)
[2] 陈涛,赵鑫,黄凯.江黄颡鱼肌肉氨基酸组成及营养分析[J].湖北农业科学,2011,50(21):4448-4450. CHEN T,ZHAO X,HUANG K.Analysis of the contents of amino acids and nutrition in muscle of Peltebagrus vachelli[J].Hubei Agricultural Sciences,2011,50(21):4448-4450.(in Chinese)
[3] 黄钧,陈琴,陈意明,等.黄颡鱼的含肉率及肌肉营养价值研究[J].广西农业生物科学,2001,1:45-50. HUANG J,CHEN Q,CHEN Y M,et al.Study on flesh content and its nutritive value of Pseudobagrus fulvidraco (Richardson)[J].Journal of Guangxi Agricultural and Biological Science,2001,1:45-50.(in Chinese)
[4] 梁正生,黄钧,施金谷,等.黄颡鱼腹水病病原菌的分离鉴定及药敏试验[J].南方农业学报,2012,43(9):1400-1404. LIANG Z S,HUANG J,SHI J G,et al.Isolation and identification ascites disease pathogen from Pelteobagrus fulvidraco Richardson and its drug sensitivity test[J].Journal of Southern Agriculture,2012,43(9):1400-1404.(in Chinese)
[5] 赵静,王利.黄颡鱼感染小肠结肠炎耶尔森氏菌的组织病理学观察[J].中国畜牧兽医,2014,41(12):254-257. ZHAO J,WANG L.Histopathological observation of Pelteobagrus fulvidraco infected with Yersinia enterocolitica[J].China Animal Husbandry & Veterinary Medicine,2014,41(12):254-257.(in Chinese)
[6] 隗黎丽,吴华东,阮记明.黄颡鱼鮰爱德华氏菌的分离及鉴定[J].江西农业大学学报,2014,36(1):187-192. WEI L L,WU H D,RUAN J M.Isolation and identification of Edwardsiella ictaluri from Yellow catfish Pelteobagrus fulvidraco[J].Acta Agriculturae Universitatis Jiangxiensis,2014,36(1):187-192.(in Chinese)
[7] 徐进,罗晓松,曾令兵.黄颡鱼鲇爱德华氏菌的分离鉴定及其致病性研究[J].淡水渔业,2009,39(6):47-53. XU J,LUO X S,ZENG L B.Isolation,identification and pathogenicity of Edwardsiella ictaluri from Chinese Yellow catfish (Pelteobagrus fulvidraco)[J].Freshwater Fisheries,2009,39(6):47-53.(in Chinese)
[8] 黄钧,温华成,施金谷,等.黄颡鱼体表溃疡病病原菌分离鉴定及药敏试验[J].南方农业学报,2012,43(1):107-112. HUANG J,WEN C H,SHI J G,et al.Isolation and identification pathogenic bacteria from Pelteobagrus fulvidraco ulcerative syndrome and its drug sensitive test[J].Journal of Southern Agriculture,2012,43(1):107-112.(in Chinese)
[9] 罗玉双,夏维福,刘良国,等.黄颡鱼出血性水肿病诊治[J].科学养鱼,1999,12:26. LUO Y S,XIA W F,LIU L G,et al.Diagnosis and treatment of hemorrhagic edema of Yellow catfish[J].Scientific Fish Farming,1999,12:26.(in Chinese)
[10] 王文彬,夏维福,罗玉双,等.黄颡鱼的寄生虫病及其防治[J].内陆水产,2001,7:34-35. WANG W B,XIA W F,LUO Y S,et al.Parasitic diseases of Pelteobagrus fulvidraco and control[J].Current Fisheries,2001,7:34-35.(in Chinese)
[11] 吴萍,崇加荣.黄颡鱼养殖中常见疾病及其防治[J].水利渔业,2003,2:65-66. WU P,CHONG J R.Prevention and treatment of common disease of Pseudobagrus vachelli[J].Reservoir Fisheries,2003,2:65-66.(in Chinese)
[12] 冯辉,何云崇.黄颡鱼鱼病的防治技术[J].动物科学与动物医学,2002,3:57-58. FENG H,HE Y C.Control technology of Pseudobagrus vachelli[J].Animal Science and Animal Medicine,2002,3:57-58.(in Chinese)
[13] 东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001. DONG X Z,CAI M Y.Common Bacterial System Identification Manual[M].Beijing:Science Press,2001.(in Chinese)
[14] 马勋,欧阳志明,陈怀青,等.迟缓爱德华氏菌对HEp-2细胞的侵袭特性[J].微生物学报,1998,5:336-340. MA X,OUYANG Z M,CHEN H Q,et al.Internalization and replication of Edwardsiella tarda in HEp-2 cells[J].Acta Microbiologica Sinica,1998,5:336-340.(in Chinese)
[15] 刘玉平,张晓峰.抗生素滥用的危害及防范[J].中外医疗,2012,31(1):188-189. LIU Y P,ZHANG X F.Harm and prevention of antibiotic abuse[J].China Foreign Medical Treatment,2012,31(1):188-189.(in Chinese)
[16] TABER H W,MUELLER J P,MILLER P F,et al.Bacterial uptake of aminoglycoside antibiotics[J].Microbiological Reviews,1987,51(4):439.
[17] HOUGHTON J L,GREEN K D,CHEN W,et al.The future of aminoglycosides:The end or renaissance?[J].Chembiochem,2010,11(7):880-902.
[18] ALEKSHUN M N,LEVY S B.Molecular mechanisms of antibacterial multidrug resistance[J]. Cell,2007,128(6):1037-1050.
[19] MAGNET S,BLANCHARD J S.Molecular insights into aminoglycoside action and resistance[J].Chemical Reviews,2005,105(2):477-498.
[20] RAMIREZ M S,TOLMASKY M E.Aminoglycoside modifying enzymes[J].Drug Resistance Updates,2010,13(6):151-171.
[21] 王燕,张晓华,吕俊超,等.养殖大菱鲆病原菌迟缓爱德华氏菌的分离鉴定及其疫苗研制[J].中国水产科学,2009,16(3):394-403. WANG Y,ZHANG X H,LV J C,et al.Isolation of Edwardsiella tarda from diseased turbot (Scophthalmus maximus)and vaccination against Edwardsiella tarda[J].Journal of Fishery Sciences of China,2009,16(3):394-403.(in Chinese)
[22] 陈言峰,卓孝磊,王超,等.鲮鱼致病性迟钝爱德华氏菌的鉴定及药敏分析[J].水产科学,2017,36(1):48-53. CHEN Y F,ZHUO X L,WANG C,et al.Identification and analysis of antibiotic sensitivity of pathogenic bacterium Edwardsiella tarda from cultured mud carp (Cirrhinus molitorella)[J].Journal of Fishery Sciences,2017,36(1):48-53.(in Chinese)
[23] 赵飞,邹为民,谭爱萍,等.地图鱼迟钝爱德华氏菌病病原菌的鉴定及毒力基因的检测[J].大连水产学院学报,2007,22(6):403-408. ZHAO F,ZOU W M,TAN A P,et al.Identification and detection of virulence gene of the pathogenic bacteria Edwardsiellosis in oscar Astronotus ocellatus[J].Journal of Dalian Fisheries University,2007,22(6):403-408.(in Chinese)

Isolation and Identification of Edwardsiella tarda in Pelteobagrus fulvidraco and Analysis of Its Pathogenicity

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.
[2]	LI Zhijie, ZHAO Ying, MA Xin, WANG Meng, YU Sijiu, WANG Libin, PAN Yangyang. Research Progress on Isolation Methods and Main Reproductive Functions of Exosomes from Animal Follicular Fluid [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1480-1488.
[3]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[4]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[5]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[6]	ZHONG Huachen, WANG Lifang, GUO Chenyang, LIU Jialin, SONG Jie. Isolation,Identification and Drug Resistance Analysis of Pathogenic Bacteria from Milk Samples and Environment of Mastitis in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 817-826.
[7]	SUN Yu, ZHANG Hao, LI Changyang, TAO Yanzi, WANG Qiuju. Isolation,Identification and Biological Characteristics of Antioxidant Bacillus from Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 359-367.
[8]	WEI Jin, WANG Jia, ZHAO Haoran, LIU Bo, LIU Yuanjie, YAO Wensheng, LIU Yan, MA Xin. Isolation, Identification and Antimicrobial Susceptibility of Mycoplasma gallisepticum from Different Areas [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 368-376.
[9]	YANG Fumei, YANG Linfu, ZENG Cheng, YANG Zhi, WANG Kang, DUAN Gang, DAI Feiyan. Isolation,Identification and Biological Characterization Analysis of a Strain of Carnobacterium maltaromaticum from Barking Deer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 398-407.
[10]	ZHANG Wei, WU Hua, YIN Yajie, LI Songli, HOU Shaohua. Isolation and Identification of Novel Duck Reovirus and Its σC Gene Sequence Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3520-3529.
[11]	XI Jing, YI Jihai, WANG Yueli, XU Zhenyu, LI Peidong, ZHANG Jiangwei, CHEN Chuangfu. Isolation, Identification and Genotype Analysis of Group A Bovine Rotavirus G10 Strain XJ-2022 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3530-3538.
[12]	LIU Xiaolan, LIU Changjin, YU Wenyang, LI Xiaoxiang, BIAN Yanchao, HUANG Xiaohua, LUO Feng, DENG Shunzhou. Isolation and Identification of Porcine Rotavirus Jiangxi Strain AY01 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3151-3162.
[13]	XU Minsheng, KE Haiyi, SHI Keda, YANG Dongxia, ZHAI Shaolun, ZANG Yingan, LI Chunling. Isolation and Identification of Actinobacillus pleuropneumoniae from Guangdong Province and Detection and Analysis of Drug Resistance Phenotype and Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3212-3225.
[14]	YANG Zemin, LI Shuang, JIN Zhengyu, LIAO Yixiao, YANG Ying, WEN Ming. Isolation,Identification and Biological Characteristics of a Strain of Lactobacillus plantarum from Carp [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2805-2811.
[15]	YIN Yajie, LIANG Ruiying, CUI Huan, MENG Lijia, NI Weiling, QIAO Sina, GUO Kangkang, ZHANG Bo, LI Songli, HOU Shaohua, DONG Shishan. Isolation,Identification of Duck Tembusu Virus,and Genetic and Evolutionary Analysis of Its Envelope Protein [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1497-1506.