嗜酸乳杆菌发酵中药对产肠毒素大肠杆菌K88所致腹泻的防治作用

doi:10.16431/j.cnki.1671-7236.2018.03.030

Abstract

Abstract:

The experiment was conducted to investigate the effects of Lactobacillus acidophilus fermented traditional Chinese medicine on diarrhea mice induced by enterotoxigenic Escherichia coli (ETEC) K88.100 6-8 weeks old BALB/c mice were chosen and divided into 4 groups:Groups Ⅰ,Ⅱ,Ⅲ and Ⅳ.Group Ⅰ was the blank control group,while the mice in groups Ⅱ,Ⅲ and Ⅳ were induced by ETEC K88.The mice in groups Ⅰ and Ⅱ were fed with basal diet without any medicine,while that in groups Ⅲ and Ⅳ were fed with basal diet supplemented with 10% traditional Chinese medicine fermentation liquor and 10% traditional Chinese medicine aqueous extract,respectively.The diarrhea rate,diarrhea index,immune organ index,intestinal flora and cytokine IFN-γ and IL-4 level were statistically analyzed.The results showed that,after 36 h of K88 infection,the diarrhea index (P < 0.05) and diarrhea rate of groups Ⅲ and Ⅳ were decreased compared with group Ⅱ,and the diarrhea index of group Ⅲ were significantly lower than group Ⅳ (P < 0.05),and the number of Escherichia coli in the cecum was decreased,while the number of Bifidobacteria and Lactobacillus bacteria were increased.The immune indexes of groups Ⅲ and Ⅳ were higher than that of groups Ⅰ and Ⅱ.Compared with group Ⅱ,the spleen index,thymus index and liver index of group Ⅲ were increased by 48.37%,29.57% and 36.88%,respectively (P < 0.05).Compared with group Ⅱ,the IFN-γ/IL-4 ratio of groups Ⅲ and Ⅳ was increased,and that of group Ⅲ was higher than group Ⅳ.In conclusion,Lactobacillus acidophilus fermented traditional Chinese medicine could reduce the diarrhea rate and diarrhea index,improve the index of immune organs,maintain the balance of intestinal flora and eliminate the inflammatory reaction in diarrhea,providing reference for the development of products for piglet diarrhea induced by ETEC.

Key words: Lactobacillus acidophilus; fermented Chinese medicine; enterotoxigenic Escherichia coli(ETEC); diarrhea rate

CLC Number:

S816.7

GU Wei, WANG Lirong, SUN Mingjie, CHEN Jing, CHEN Zhen, ZENG Jiajia, LI Shidong, WANG Chunfeng, SHAN Baolong, XU Haiyan. Effect of Lactobacillus acidophilus Fermentated Traditional Chinese Medicine on Diarrhea Induced by Enterotoxigenic Escherichia coli K88[J]. , 2018, 45(3): 798-806.

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Effect of Lactobacillus acidophilus Fermentated Traditional Chinese Medicine on Diarrhea Induced by Enterotoxigenic Escherichia coli K88

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