假蒟提取物的体外抗氧化和抗炎效果研究

doi:10.16431/j.cnki.1671-7236.2019.03.005

Abstract

Abstract:

This study was aimed to investigate the active ingredient and evaluate the antioxidant and anti-inflammatory activities of Piper sarmentosum Roxb. extracts in vitro.The antioxidant activities of three pepper extracts (petroleum ether,n-butanol and ethyl acetate) in vitro were determined by ABTS method.The inflammation model of IPEC-J2 cell was established with LPS in vitro,the levels of tumor necrosis factor (TNF-α),interleukin-1 (IL-1) and interleukin-6 (IL-6) were detected in cell supernatant by ELISA after pretreatment of Piper sarmentosum Roxb. extract.The results showed that the total antioxidant activity of Piper sarmentosum Roxb. extract in descending order were n-butanol extract,ethyl acetate extract and petroleum ether extract (P<0.05).The contents of total alkaloids and total phenols of Piper sarmentosum Roxb. n-butanol extract were the highest,which were 2.81 mEq/100 g and 161.82 mg/g,respectively.In anti-inflammatory experiments,compared with the blank group,the levels of TNF-α(P<0.05),IL-1(P>0.05) and IL-6 (P<0.05) in IPEC-J2 cells increased in LPS group,while compared with the Piper sarmentosum Roxb. pretreatment groups,these indexes in LPS group decreased significantly (P<0.05).The concentrations of cytokines from high to low were ethyl acetate extract,petroleum ether extract and n-butanol extract.In conclusion,the extract of Piper sarmentosum Roxb. had antioxidant activity,and it could affect the secretion of cytokines in IPEC-J2 cells and inhibit the inflammation.

Key words: Piper sarmentosum Roxb.; pig intestinal epithelial cells; antioxidant activity; anti-inflammatory

CLC Number:

S816.7

CHEN Chuanwei, ZHOU Luli, WANG Dingfa, ZHOU Hanlin. Study on Antioxidant and Anti-inflammatory Effects of Extracts from Piper sarmentosum Roxb. in vitro[J]. , 2019, 46(3): 677-683.

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Study on Antioxidant and Anti-inflammatory Effects of Extracts from Piper sarmentosum Roxb. in vitro

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