牛病毒性腹泻病毒、大肠杆菌和奇异变形杆菌混合感染致犊牛腹泻的研究

doi:10.16431/j.cnki.1671-7236.2018.01.024

Abstract

Abstract:

In order to determine the cause of calves diarrhea and to provide appropriate treatment and prevention measures,13 anal swab samples and 13 serum samples were taken from calves with diarrhea in a scale dairy farm in Linxia of Gansu province. Identification of antibodies to bovine viral diarrhea virus in serum and pathogenic bacteria in diarrhea feces was performed by means of colloidal gold technology, ELISA, bacterial isolation and identification, and Kirby-Bauer method. The virology test results showed that the antigens of bovine rotavirus (BRV) and bovine coronavirus (BCV) were not detected in 13 fecal samples, however the positive rate of bovine viral diarrhea virus (BVDV) was 23.08%(3/13); No antibodies to BRV and BCV were detected in 13 serum samples, whereas the positive rate of BVDV serological antibody was 38.46%(5/13).Pathogen test results showed that 13 strains of Escherichia coli and 7 strains of Proteus mirabilis were isolated from 13 stool samples. Drug sensitivity test showed that isolated Escherichia coli and Proteus mirabilis had different degrees of resistance to 20 conventional drugs; No drug was found to be effective against both of the bacteria at the same time. The calves diarrhea was caused by mixed infection of BVDV, Escherichia coli and Proteus mirabilis. Escherichia coli and Proteus mirabilis were highly resistant to drugs, which provided an effective treatment basis for calves diarrhea in the cattle farm.

Key words: calves diarrhea; bovine viral diarrhea virus (BVDV); Escherichia coli; Proteus mirabilis; drug sensitivity test

CLC Number:

S823

WANG Danyang, WANG Xurong, ZHANG Kang, WANG Hairui, WANG Lei, ZHANG Jingyan, ZHANG Kai, LI Jianxi, WANG Xuezhi. Study on Calves Diarrhea Caused by Mixed Infection of Bovine Viral Diarrhea Virus, Escherichia coli and Proteus mirabilis[J]. , 2018, 45(1): 189-195.

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Study on Calves Diarrhea Caused by Mixed Infection of Bovine Viral Diarrhea Virus, Escherichia coli and Proteus mirabilis

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