一株猪伪狂犬病病毒变异株的分离鉴定

doi:10.16431/j.cnki.1671-7236.2018.01.025

Abstract

Abstract:

The purpose of this study was to understand the genetic variation and evolution of pseudorabies virus (PRV) in Guangdong province.In this experiment,the PRV strain was primarily identified by PCR from samples (heart,lung,tonsil,liver and spleen) collected from sick pig in Foshan,Guangdong province,and the positive samples were inoculated Vero cells for subculture. And a strain was identifited as PRV by PCR and mice test,and named as PRV FS-2015.The cytopathic effect of the strain was observed and TCID₅₀ was tested,the whole gene of TK and gC were amplified and the sequence were analyzed. The results showed that the titer of TCID₅₀ of isolated virus in Vero cells was 10^-7.5/0.1 mL.The sequence analysis results showed that the nucleotide identities of the gC and TK genes of PRV FS-2015 strain with other PRV strains in GenBank were 95.8% to 100.0% and 99.4% to 100.0%,respectively,the homology of amino acid sequence were 92.3% to 100.0% and 98.7% to 100.0%,respectively. Phylogenetic tree analysis showed that the PRV FS-2015 stain had closer relationship with GY,ZJ01,HB1201,HN1201,JS2012,BJ/YT and BP stains,while had a distant relationship with Kaplan, Becker,NIA3,Kolchis,Bartha,Yangsan,Min-A,SS and SL stains. The experiment showed that PRV FS-2015 strain belonged to the variant popular in recent years,and the results could provide data for the prevention and control of pseudorabies and the selection of vaccine strains in Guangdong province.

Key words: pseudorabies virus (PRV); isolation and identification; sequence analysis; gC gene; TK gene; genetic variation

CLC Number:

S852.65

PANG Xuanfei, LIAN Sinan, LI Zhongsheng, WAN Zengpei, BAI Aiquan. Isolation and Identification of a Mutant Strain of Pseudorabies Virus[J]. , 2018, 45(1): 196-205.

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Isolation and Identification of a Mutant Strain of Pseudorabies Virus

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