关中奶山羊PIS区域序列多态性分析

doi:10.16431/j.cnki.1671-7236.2017.12.023

Abstract

Abstract:

Polled trait of goat is important economic trait in livestock production,goat polled intersex syndrome (PIS) influenced polled phenotype and intersex phenotype of goats. PIS sequence locats in a approximately 11.7 kb nucleotide sequence in chromosome 1. This experiment was aimed to study the effect of PIS sequence on polled phenotype in Guanzhong dairy goats (3 horned goats, 3 polled goats). The PIS whole sequence in Guanzhong dairy goats were amplified using four pair of primers (PIS1-1, PIS1-2, PIS2 and PIS3), the whole absence of PIS sequence was detected using the fifth primer (PIS whole). The sequencing result was assembled, analyzed and annotated. The result showed that the PIS sequence full length was 12.814 kb, and the BLAST alignment result was correct. PIS whole primer didn't amplify any srtipes, which showed that there was no a total deletion of 11.7 kb in Guanzhong dairy goats. SeqMan software analysis result showed that there was a total of 30 polymorphic sites, wihch were likely to connect with polled/horned phenotype in Guanzhong dairy goats. The annotation result of concatenation sequence showed that there were one tRNA encoding locus, two microsatellites, three microRNA encoding loci, L1-EN conserved domain, RT-nLTR-like conserved domain, also find a CpG island, one pair of inverted repeats, and two tandem repeats. This experiment result had a significant reference value for studying the molecular mechanism of polled phenotype in Guanzhong dairy goats.

Key words: Guanzhong dairy goat; polled trait; polled intersex syndrome (PIS); polymorphism; sequence annotation

CLC Number:

S827.9⁺4

HOU Ce, SU Xiao-ping, WANG Qi-tian, CUI Kui-qing, SHI De-shun, LIU Qing-you. Polymorphism Analysis of PIS Region Sequence in Guanzhong Dairy Goat[J]. , 2017, 44(12): 3554-3562.

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Polymorphism Analysis of PIS Region Sequence in Guanzhong Dairy Goat

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