中甸牦牛mtDNA Cytb基因遗传多样性及系统进化分析

doi:10.16431/j.cnki.1671-7236.2016.10.025

Abstract

Abstract:

In order to investigate the genetic diversity and the origin of evolutionary relationship of Zhongdian yak,we analyzed the complete sequence of 15 individuals Cytb gene,its sequence polymorphism was analyzed,and the phylogenetic tree was constructed.The results showed that the length of the nucleotide sequence were 1 140 bp,with nucleotide frequencies of 26.3%,31.8%,13.1% and 28.8% for T,A,G and C,respectively.Three haplotypes were identified of 15 individuals,with 3 polymorphic sites,including two conversions,one transversion,haplotype diversity was 0.2571 and nucleotide diversity was 0.00035.Phylogenetic analysis suggested that Zhongdian yak and Bos mutusc clustered firstly,then gathered with Bison bison,which indicated that there were high genetic similarity and closer genetic relationship,genetic similarity with other cattle genus was relatively low.Combining with the proof of molecular biology and paleontology,the result supported the point that Bos grunniens and Bos mutus were classified as an alone genus in Bovinae.

Key words: Zhongdian yak; Cytb gene; phylogenetic evolution; genetic diversity

CLC Number:

S823

TU Shi-ying, SONG Na-na, CHAI Zhi-xin, ZHONG Jin-cheng. Analysis on Genetic Diversity and Phyletic Evolution of mtDNA Cytb Gene in Zhongdian Yak[J]. , 2016, 43(10): 2680-2687.

References

[1] 和绍禹,田允波,葛长荣,等.中甸牦牛[J].黄牛杂志,1998,24(2):22-25.
[2] 刘国樑.中甸牦牛[J].中国牦牛,1980,2:2.
[3] 杨晓峰,松耀武,墨继光,等.中甸牦牛的起源及开发利用[J].养殖与饲料,2010,12:103-104.
[4] 孙玉鹏.中甸牦牛育肥关键技术[J].养殖与饲料,2010,3:3-4.
[5] 雷晓琴,郭成裕,马文志.云南中甸牦牛的种质特性研究[J].当代畜牧,2012,9:40-41.
[6] Hatefi Y.The mitochondrial electron transport and oxidative phosphorylation system[J].Annual Review of Biochemistry,1985,54(1):1015-1069.
[7] Johns G C,Avise J C.A comparative summary of genetic distances in the vertebrates from the mitochondrial cytochrome b gene[J].Molecular Biology and Evolution,1998,15(11):1481-1490.
[8] Paulus K B,Tiedemann R.Ten polymorphic autosomal microsatellite loci for the Eider duck Somateria mollissima and their cross-species applicability among waterfowl species (Anatidae)[J].Molecular Ecology Notes,2003,3(2):250-252.
[9] Denk A G,Gautschi B,Carter K,et al.Seven polymorphic microsatellite loci for paternity assessment in the mallard (Anas platyrhynchos)[J]. Molecular Ecology Notes,2004,4(3):506-508.
[10] 潘宝平,卜文俊.线粒体基因组的遗传与进化研究进展[J].生物学通报,2005,40(8):1-3.
[11] 田应华,钱林东,凌军,等.云南中甸牦牛的遗传多样性研究[J].西南农业学报,2009,22(3):794-797.
[12] 杨雪娇,任战军,张成东,等.基于Cytb基因多态性对藏獒群体遗传结构及系统进化的研究[J].畜牧兽医学报,2014,45(7):1068-1074.
[13] 海棠,黄金龙,赵一萍,等.蒙古家马和蒙古野马线粒体细胞色素b基因遗传多样性及系统进化分析[J].中国畜牧兽医,2014,41(6):162-167.
[14] 马国龙,常洪,李世平,等.以Cytb基因部分序列分析大额牛系统地位及其种群现状[J].遗传学报,2007,34(5):413-419.
[15] 蔡欣,陈宏,雷初朝,等.中国3个牛种Cytb 基因多态性及其系统发育研究[J].西北农林科技大学学报:自然科学版,2007,35(2):43-46.
[16] 姬秋梅,唐懿挺,张成福,等.西藏牦牛mtDNA Cytb基因的序列多态性及其系统进化分析[J].畜牧兽医学报,2012,43(11):1723-1732.
[17] 李齐发,李隐侠,赵兴波,等.牦牛线粒体DNA细胞色素b基因序列测定及其起源,分类地位研究[J].畜牧兽医学报,2006,37(11):1118-1123.
[18] 杨万远,陈雪梅,钟金城,等.野牦牛mtDNA Cytb 基因全序列测定及系统进化关系[J].中国草食动物,2009,3:8-13.
[19] Thompson J D,Gibson T J,Plewniak F,et al.The CLUSTAL_X windows interface:Flexible strategies for multiple sequence alignment aided by quality analysis tools[J].Nucleic Acids Research,1997,25(24):4876-4882.
[20] Tamura K,Dudley J,Nei M,et al.Mega4:Molecular evolutionary genetics analysis (Mega) software version 4.0[J].Molecular Biology and Evolution,2007,24(8):1596-1599.
[21] Rozas J,Sánchez-Del Barrio J C,Messeguer X,et al.DnaSP,DNA polymorphism analyses by the coalescent and other methods[J].Bioinformatics,2003,19(18):2496-2497.
[22] Wu X,Chu M,Liang C,et al.The complete mitochondrial genome sequence of the Datong yak (Bos grunniens)[J].Mitochondrial DNA,2016,27(1):433-434.
[23] Chu M,Wu X,Liang C,et al.The complete sequence of mitochondrial genome of polled yak (Bos grunniens)[J].Mitochondrial DNA A DNA Mapp Seq Anal,2016,27(3):2032-2033.
[24] 常国斌,常洪,陈国宏,等.基于Cytb基因部分序列分析巴州牦牛遗传多样性及其系统地位[J].中国畜牧杂志,2010,17:19-21.
[25] Miyamoto M M,Tanhauser S M,Laipis P J.Systematic relationships in the artiodactyl tribe Bovini (family Bovidae),as determined from mitochondrial DNA sequences[J].Systematic Biology,1989,38(4):342-349.
[26] Kraus F,Jarecki L,Miyamoto M M,et al.Mispairing and compensational changes during the evolution of mitochondrial ribosomal RNA[J].Mol Biol Evol,1992,9:770-774.
[27] 李齐发,李艳华,赵兴波,等.牛亚科MHC-DRB3 exon 2的序列变异分析[J].农业生物技术学报,2005,13(4):441-446.

Analysis on Genetic Diversity and Phyletic Evolution of mtDNA Cytb Gene in Zhongdian Yak

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