马可·波罗盘羊和5种家养绵羊MHC-DRB3基因第2外显子遗传多态性分析

doi:10.16431/j.cnki.1671-7236.2022.03.018

Abstract

Abstract: 【Objective】 This study was aimed to analyze the polymorphism of MHC-DRB3 gene in Ovis ammon polii, determine its allele number, nucleotide and amino acid polymorphic loci, as well as genetic relationship and evolutionary relationship with domestic sheep.【Method】 PCR-SSCP method was used to detect the alleles of MHC-DRB3 gene exon 2 in 39 Ovis ammon polii, 159 Taxkorgan sheep, 72 Tibetan sheep, 76 Ningxia Tan sheep, 36 Kirgiz sheep and 26 Duolang sheep.The differential alleles of different sheep breeds were cloned and sequenced, and the haplotype sequences of alleles were analyzed.The physical and chemical properties, structure and function of MHC-DRB3 gene exon 2 coded protein in Ovis ammon polii were analyzed by bioinformatics online softwares.【Result】 This study obtained 66 MHC-DRB3 gene alleles, including 23 alleles for Ovis ammon polii, 14 alleles for Taxkorgan sheep, 12 alleles for Tibetan sheep, 12 alleles for Ningxia Tan sheep, 4 alleles for Kirgiz sheep, and 1 allele for Duolang sheep.The number of nucleotide polymorphic loci of allele sequence of MHC-DRB3 gene exon 2 in Ovis ammon polii, Taxkorgan sheep, Tibetan sheep, Ningxia Tan sheep and Kirgiz sheep were 74, 76, 45, 49 and 25, respectively.It showed that Ovis ammon polii and Taxkorgan sheep had higher polymorphisms than Tibetan sheep, Ningxia Tan sheep and Kirgiz sheep.The phylogenetic analysis results showed that the MHC-DRB3 gene of Ovis ammon polii and Taxkorgan sheep both diverged into two branches.The MHC-DRB3 gene haplotypes of Ovis ammon polii, Taxkorgan sheep and Tibetan sheep had high similarity.The product encoded by MHC-DRB3 gene exon 2 of Ovis ammon polii was an unstable hydrophilic protein.The secondary structure mainly composed of random coil and alpha helix, and its biological effects were mainly reflected in cytoplasm.【Conclusion】 In this study, the number of alleles of MHC-DRB3 gene exon 2 and the polymorphic loci of nucleotide and amino acid of MHC-DRB3 gene in Ovis ammon polii and five species of domestic sheep were determined, it provided materials for further exploring the regulatory mechanism and evolutionary significance of MHC-DRB3 gene in sheep.

Key words: Ovis ammon polii; domestic sheep; MHC-DRB3 gene; PCR-SSCP; genetic polymorphism

CLC Number:

S813.3

ZHANG Yonghao, XIAO Yi, GUO Lijun, Tuerxun·Abudoureheman, WANG Yutao. Genetic Polymorphism Analysis of MHC-DRB3 Gene Exon 2 in Ovis ammon polii and Five Domestic Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 962-972.

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Genetic Polymorphism Analysis of MHC-DRB3 Gene Exon 2 in Ovis ammon polii and Five Domestic Sheep

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