荧光标记微卫星分析广西树鼩群体的遗传多态性

doi:10.16431/j.cnki.1671-7236.2016.01.027

Abstract

Abstract: A total of 10 fluorescent labeling microsatellite markers detected by capillary electrophoresis were selected to analyze the genetic diversity of tree shrew(T.belangeri chinensis).Microsatellite Toolkit and PopGene softwares were used to calculate the genetic correlation indexes to assess the genetic diversity of the population.64 alleles with 6.4 alleles per locus were identified among 10 microsatellite loci.The average effective number of alleles, observed heterozygosity, expected heterozygosity and polymorphic information content were 3.7892, 0.6156, 0.6963, and 0.6367, respectively.Seven of all loci were high polymorphic, and three were moderate polymorphic.Five loci extremely significantly deviated from Hardy-Weinberg equilibrium(P<0.01).These novel polymorphic microsatellite markers indicated high genetic diversity, which will be used to evaluate genetic diversity in tree shrew.

Key words: tree shrew(T.belangeri chinensis); capillary electrophoresis; microsatellite DNA; genetic polymorphism

CLC Number:

S813.3

YANG Xiang-di, CAO Ji, YANG Chun, LI Yuan, ZHOU Ling-li, LUO Wang, WANG Gu-yang, LI Ke-zhi. The Genetic Polymorphism Analysis in Tree Shrew(T.belangeri chinensis) based on Fluorescent Microsatellite[J]. , 2016, 43(1): 182-190.

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The Genetic Polymorphism Analysis in Tree Shrew(T.belangeri chinensis) based on Fluorescent Microsatellite

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