麦洼牦牛基因组三碱基重复微卫星的挖掘及其遗传多态性分析

doi:10.16431/j.cnki.1671-7236.2017.05.026

Abstract

Abstract:

In order to study the genetic diversity and differentiation characteristics of the existing population of Maiwa yak and provide a theoretical basis for utilization in the future, a total of 9 466 tri-nucleotide repeats microsatellites loci were screened based on the release of genome of yaks by bioinformatics method. The tri-nucleotide repeats microsatellites were amplified by PCR using the genomic DNA of 191 Maiwa yaks as templates,and the polymorphic microsatellite loci were genotyped through polyacrylamide electrophoresis experiment. After the Hardy-Weinberg equilibrium test, 5 tri-nucleotide microsatellite loci were deviated from the Hardy-Weinberg equilibrium (P <0.05). 15 microsatellite loci were highly polymorphic (PIC >0.5), the mean observed heterozygosity (Ho),mean expected heterozygosity(He) and mean polymorphic information content(PIC) were 0.5041,0.8152 and 0.7831,respectively. Maiwa yak population exhibited no characteristics of differentiation by Structure 3.0 software analysis. Therefore, Maiwa yak had plentiful genetic polymorphism while no signs of genetic differentiation were observed within the population.

Key words: Maiwa yak; tri-nucleotide; microsatellite loci; genetic polymorphism

CLC Number:

S823.8⁺5

LUO Hui, ZHAO Fang-fang, SUN Lei, WU Shi-xin, XU Chuan-fei, CAI Xin. Screening the Polymorphic Tri-nucleotide Repeat Microsatellites from the Genome of Maiwa Yak and Their Genetic Diversity Analysis[J]. , 2017, 44(5): 1438-1445.

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Screening the Polymorphic Tri-nucleotide Repeat Microsatellites from the Genome of Maiwa Yak and Their Genetic Diversity Analysis

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