麦洼牦牛G蛋白信号转导调节因子5克隆及蛋白结构分析

doi:10.16431/j.cnki.1671-7236.2015.01.006

Abstract

Abstract: Regulator of G protein signaling 5 was cloned and analyzed by RT-PCR and bioinformatic methods.Phylogenetic tree was constructed using ClustalX 1.83 and Mega 5.0.The results showed that the sequence of RGS5 was 863 bp and contained an open reading frame of 546 bp, which encoded 181 amino acids.Phylogenetic tree indicated that amino acids of RGS5 in Maiwa yak had high homology with cattle.The molecular mass of RGS5 protein was 20.94 ku and the theoretical PI was 6.35.The RGS5 was an unstable protein.It mainly contained helix with no signal peptide and transmembrane domain.Tertiary structure of RGS5 protein of Maiwa yak had similar tertiary structure with human RGS5 protein.It would be benefit for further study on function of RGS in Maiwa yak.

Key words: Maiwa yak; regulator of G protein signaling 5; cloning; protein analysis

CLC Number:

Q785

ZHAO Jing, WANG Li, LI Jian, ZI Xiang-dong, ZHONG Jin-cheng, JIANG Ming-feng, WANG Hai-juan, LI Xue-feng. Cloning and Protein Structure Analysis of Regulator of G Protein Signaling 5 in Maiwa Yak[J]. , 2015, 42(1): 38-43.

References

[1] 袁有清,孙福勇.麦洼牦牛品种资源的保护和开发利用[J].阿坝科技,2013,90(1):4-6.
[2] 刘仲娜,钟金城,柴志欣,等.麦洼牦牛SRY基因克隆及序列分析[J].中国畜牧兽医,2013,45(12):5-11.
[3] Xiao B,Zhang Y,Niu W Q,et al.Haplotype-based association of regulator of G-protein signaling 5 gene polymorphisms with essential hypertension and metabolic parameters[J].Clin Chem Lab Med,2009,47(12):1483-1488.
[4] Yang T,Terman J R.Regulating small G protein signaling to coordinate axon adhesion and repulsion[J].Small GTPases,2013,4(1):34-41.
[5] 尹琳琳,朱兴族.G蛋白信号调节蛋白作为中枢神经系统药物新靶点研究进展[J].中国药理学通报,2005,21(9):1041-1045.
[6] 何明珠.麦洼牦牛、九龙牦牛种质资源特性及保护措施[J].草业与畜牧,2012,201(8):42-46.
[7] 马晓琴,黄林,刘薇,等.麦洼牦牛乳酸脱氢酶-B基因多态性的PCR-RFLP分析[J].四川动物,2013,32(2):192-194.
[8] DiBello P R,Garrison T R,Apanovitch D M,et al.Selective uncoupling of RGS action by a single point mulation in the G protein α-subunit[J].J Biol Chem,1998,273(10):5780-5784.
[9] 黄维荪,王靖华.G蛋白信号调节蛋白5在肿瘤血管正常化中的作用[J].医学研究生学报,2010,23(10):1090-1092.
[10] Li H L,He C W,Feng J H,et al.Regulator of G protein signaling 5 protects against cardiac hypertrophy and fibrosis during biomechanical stress of pressure overload[J].Proc Natl Acad Sci USA,2010,107(31):13818-13823.
[11] Berqer M,Berqer G,Arnold B,et al.Regulator of G-protein signaling-5 induction in pericytes coincides with active vessel remodeling during neovascularization[J].Blood,2005,105(3):1094-1101.
[12] 谢志华,张德昌.RGS蛋白在G蛋白信号转导中的作用与调节[J].国外医学分子生物学分册,2002,24(2):93-97.
[13] Burchett S A.Regulators of G protein signaling: A bestiary of modular protein binding domains[J].J Neurchem,2000,75(4):1335-1351.
[14] Zhou J,Kayoko M,Nishiyama M,et al.Characterization of RGS5 in regulation of protein-coupled receptor signaling[J].Life Sci,2001,68(13):1457-1466.
[15] Seki N,Sugano S,Suzuki Y,et al.Isolation,tissue expression,and chromosomal assignment of human RGS5,a novel G-protein signaling regulator gene[J].J Hum Genet,1998,43(3):202-205.
[16] 赵朝芬,何郎,文世民.G蛋白调节信号5在肿瘤中的作用[J].国际肿瘤学杂志,2013,40(10):729-732.
[17] 史雌华.G蛋白信号调节因子在心血管系统中的作用[J].中国当代医学,2012,19(12):15-17.
[18] Moroi K,Nishiyama M,Kawabata S,et al.Phosphorylation of Ser166 in RGS5 by protein kinase C causes of RGS function[J].Life Sci,2007,81(1):40-50.
[19] Bordoli L,Kiefer F,Arnold K,et al.Protein structure homology modeling using SWISS-MODEL workspace[J].Nat Prot,2009,4(1):1-13.
[20] 王利,魏勇,龙钟明,等.齐口裂腹鱼同种移植炎症因子AIF-1的克隆及蛋白结构预测[J].西南农业学报,2012,25(2):723-727.

Cloning and Protein Structure Analysis of Regulator of G Protein Signaling 5 in Maiwa Yak

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	FAN Bingfeng, LI Wen, LIU Lixiang, ZHAO Xiangyuan, SHAO Jing, LIN Lefeng, SUN Huimin, ZHANG Xulin, XU Baozeng. Cloning and Bioinformatics Analysis of AANAT Gene in Mink [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1307-1318.
[2]	LIU Hongrun, ZHU Siran, FENG Lingli, ZHANG Kun, YAN Gang, WANG Yubin, ZHANG Shuai, JIANG Shan, XU Di, LAN Ganqiu, LIANG Jing. Cloning,Bioinformatics Analysis and Tissue Expression Localization of CDO1 Gene in Large White Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1352-1363.
[3]	ZHANG Yongjie, SUI Zhiyuan, ZHANG Zhishuai, WANG Chenguang, LI Xiaojun, XING Feng. Cloning and Bioinformatics Analysis of NPTX1 Gene and Its Tissues Expression at Puberty in Dolang Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1048-1058.
[4]	WEI Yirong, ZHENG Zihua, ZHANG Sanbao, SONG Ying, JIANG Haiyu, SUN Wenyue, CHENG Pengfei, LIU Yufan, ZOU Jianwei, HUANG Yanna, PAN Yan, JIANG Qinyang. Cloning,Bioinformatics Analysis and Tissue Expression of UCP1 Gene in Nubian Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 440-450.
[5]	TANG Jingyu, DU Hanyu, MENG Chunchun, LIU Guangqing. Cloning,Bioinformatics Analysis and Subcellular Localization of Interferon-stimulated Gene 15 in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2843-2854.
[6]	QI Nannan, QIAN Yonghang, LUO Gang, SITU Xuqi, LIU Jiying. Cloning, Bioinformatics Analysis and Tissue Expression Location of Porcine MORC2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2431-2441.
[7]	ZHANG Hang, SHA Huiyang, LI Huawei, HUANG Liangzong, ZHAO Mengmeng. Cloning, Bioinformatics and Tissue Expression Analysis of TRIM3 Gene in Swine [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2474-2483.
[8]	YAN Yelian, ZHANG Mengya, LIU Qiuchen, WANG Xin, XU Changzhi, ZONG Yanfeng, ZHU Zhihua, WU Sucheng, SONG Yu, LI Yunsheng, ZHANG Yunhai, CAO Zubing. Research Progress of Epigenetic Reprogramming in Mammalian Somatic Cell Nuclear Transfer Embryo Development [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2259-2269.
[9]	WANG Baobao, JIN Hang, XIAN Yuhan, FENG Hongsheng, GAO Fengshan. Cloning SLA-1 Gene from Porcine Kidney Epithelial Cells and Analyzing its Molecular Structure Characteristics [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1671-1678.
[10]	ZHANG Jihu, MI Renisa, WANG Zixuan, LI Lin, XING Feng, LIU Shudong. Cloning and Bioinformatics of ERα Gene and Its Expression of Ovary Tissue in Cele Black Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1679-1687.
[11]	SHEN Xiang, CHEN Linghui, YAO Jingjie, WANG Sanhu, BAI Yueyu, ZHANG Xiaojian. Cloning,Bioinformatics and Expression Analysis of Bovine CCR7 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1244-1252.
[12]	ZHANG Zhishuai, SUI Zhiyuan, ZHANG Jihu, LI Qingjin, XING Feng. Cloning of CNP Gene and Its Tissue Expression Characteristics at Puberty in Dolang Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1402-1412.
[13]	HOU Jinxing, WANG Zhanhang, ZHU Junru, JIANG Yue, LIU Shujuan, AN Xiaopeng. Cloning, Bioinformatics and Tissue Expression Profile Analysis of CSN1S1 Gene in Guanzhong Dairy Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 857-865.
[14]	WEI Yunfang, LI Feixiang, Xing Yun, Huang Qingguo, LI Judong, WAN Jiusheng, CHEN Fangliang. Study on Cloning, Bioinformatics Analysis and Spatio-temporal Expression of IGF2 Gene in Kunming Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 866-875.
[15]	GONG Shujuan, SHI Ruijun, WU Tinghui, LI Shuwei. Cloning, Prokaryotic Expression and Skin Follicles Expression of KAP11.1 Gene in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 876-886.