从江香猪抑制素α基因克隆及其卵巢表达研究

doi:10.16431/j.cnki.1671-7236.2016.01.028

Abstract

Abstract: To reveal the relationship between inhibin-α(INHA) gene and the reproductive traits of Congjiang Xiang pig, INHA gene was cloned and sequenced taking the genomic DNA of Congjiang Xiang pigs as templates by polymerase chain reaction(PCR) method.The polymorphisms of INHA gene were tested in Congjiang Xiang pig populations with high-litter size and low-litter size using allele-specific PCR(AS-PCR) method.The expression profile of INHA gene in ovaries was detected from Congjiang Xiang pigs with high-litter yiled or low-litter yiled by Real-time PCR method.The complete coding region of INHA gene was 1095 bp in length, which coded for 364 amino acid residues.Compared with the known sequence, two candidate sites, G359A and A373G, were found out from exon 2 region of INHA gene in Congjiang Xiang pig.After investigation for the two sites in a large population, the frequency of alleles between two populations was not significant and without obvious relativity with the litter yiled of Congjiang Xiang pig.However, the INHA mRNA level in the ovary of Congjiang Xiang pig with high-litter yiled was higher than that with low-litter yiled.It suggested that INHA gene was much conserved, INHA gene expression level might be concerned for the regulation of ovary growth and follicle development in Congjiang Xiang pig breed.

Key words: Congjiang Xiang pig; inhibin; gene cloning; ovary; SNP

CLC Number:

ZHANG Xiao, SU Yan, YANG Shi-bin, WANG Jia-fu, RAN Xue-qin. Cloning of Inhibin-α Gene and its mRNA Expression Pattern in the Ovary of Congjiang Xiang Pig[J]. , 2016, 43(1): 191-196.

References

[1] 李万宏,王春强,周虚.抑制素对动物生殖系统的影响及其在动物生产中的应用[J].中国畜牧兽医,2011,38(6):204-207.
[2] 孙晓萍,刘建斌,张万龙,等.不同来源抑制素抗原对牛羊繁殖力影响研究[J].黑龙江畜牧兽医,2014,24(12):43-44.
[3] Kempisty B,Jackowska M,Wozna M,et al.Expression and Cellular distribution of INHA and INHB before and after in vitro cultivation of porcine oocytes isolated from follicles of different size[J]. J Biomed Biotechnol,2012,2012:742829.
[4] Sasaki K,Medan M S,Watanabe G,et al.Immunization of goats against inhibin increased follicular development and ovulation rate[J]. J Reprod Dev,2006,52(4):543-550.
[5] 张超,罗艳梅,张家骅等.激活素、抑制素及其受体与动物生殖作用的研究进展[J].中国畜牧兽医,2011,38(2):115-118.
[6] 姜庆林,储明星,陈国宏,等.绵羊抑制素A基因外显子1多态性及其与高繁殖力关系的研究[J].安徽农业大学学报,2013,40(6):945-949.
[7] 田秀娥,孙红霞,王永军.3个绵羊群体INHA基因的遗传多态性及对产羔数的影响[J].西北农林科技大学学报,2010,38(1):23-29.
[8] 杨廷模,刘畅,赵恒鑫,等.高产从江香猪资源调查及其FSH基因型分析[J].畜牧与兽医,2014,46(8):5-8.
[9] 丁朝阳,陈斌,柳小春,等. INHA基因多态性与猪产仔数的相关性分析[J].中国畜牧杂志,2011,47(1):6-9.
[10] 赵中权,李周权,张家骅. INH α亚基基因的多态性与山羊产羔数的相关性分析[J].中国畜牧杂志,2012,48(5):11-13.
[11] 周文然,储明星,孙少华,等.小尾寒羊高繁殖力候选基因INHA的研究[J].农业生物技术学报,2007,15(1):32-36.
[12] 滑国华,陈世林,姚红卫,等.山羊抑制素α亚基基因Hae Ⅱ酶切多态性及其与产羔数性状的关联分析[J].遗传,2007,29(8):972-976.
[13] Hou J,An X,Li G,et al.Exploring polymorphisms and their effects on reproductive traits of the INHA and INH β A genes in three goats breeds[J]. Anim Sci J,2012,83(4):273-278.
[14] Zi X D,Xu H W,Wang Y.Variation in sequences and mRNA expression levels of inhibin subunits α(INHA) and βA(INHBA) gened between prolific and nonprolific goat breeds[J]. Mol Reprod Dev,2012,79(4):238.
[15] Mayo K E,Cerelli G M,Spiess J,et al.Inhibin A-subunit cDNAs from porcine ovary and human placenta[J]. Proc Nati Acad Sci USA,1986,83(16):5849-5853.
[16] Mylonas I,Makovitzky J,Richter D U,et al.Expression of the inhibin-alpha subunit in normal,hyperplastic and malignant endometrial tissue:An immunohistochemical analysis[J]. Gynecol Oncol,2004,93(1):92-97.
[17] Sirotkin A V.Cytokines:Signalling molecules controlling ovarian functions[J]. Int J Biochem Cell Biol,2011,43:857-861.
[18] Bernard D J,Chapman S C,Woodruff T K.Mechanisms of inhibin signal transduction[J]. Recent Prog Horm Res,2001,56:417-450.
[19] Chen F,Jiang X,Chen X,et al.Effects of downregulation of inhibin alpha gene expression on apoptosis and proliferation of goose granulosa cells[J]. J Genet Genomics,2007,34:1106-1113.
[20] Sharara F I,Scott R T Jr,Seifer D B.The detection of diminished ovarian reserve in infertile women[J]. Am J Obstet Gynecol,1998,179(3):804-812.
[21] Medan M S,Akagi S,Kaneko H,et al.Effects of re-immunization of heifers against inhibin on hormonal profiles and ovulation rate[J]. Reproduction, 2004,128(4):475-482.

Cloning of Inhibin-α Gene and its mRNA Expression Pattern in the Ovary of Congjiang Xiang Pig

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	MA Jifu, Adil·Ketan, XIE Jingang, Bakhet·Bodai, LI Zhenwei, Oralhazi·Hazikhan. Screening of Nanobody Specific to Inhibin α Subunit Based on Phage Display Technology [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2032-2043.
[2]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[3]	MA Jifu, BAKHET·Bodai, KEZERBEK·Kazibek, LI Zhenwei, ORALHAZI·Hazikhan. Screening and Identification of Inhibin α Subunit-specific Nanobody Gene [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1532-1542.
[4]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[5]	XU Haoqi, XU Jingyi, HU Lirong, ZHANG Fan, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, LIU Qiaoxiang, GUO Gang, WANG Yachun. Polymorphism of PIK3CB Gene and Its Association Analysis with Reproductive and Milk Production Traits in Chinese Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3438-3452.
[6]	FENG Xiaopin, LI Yinxia, ZHANG Chenjian, ZHANG Jun, MENG Chunhua, QIAN Yong, CAO Shaoxian. Coning and Polymorphism Identification of PLAG1 Gene and Its Association Analysis with Birth Weight and Body Size Traits in Boer Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3465-3474.
[7]	ZHOU Ya, ZHANG Heyang, LIU Linling, LI Haodong, ZHENG Junjun, WANG Guiwu. Analysis of FGFR2 Gene Polymorphism and Their Correlation with Antler Weight Traits in Sika Deer [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3006-3014.
[8]	HU Wen, HUO Konglin, SONG Xingxing, ZHANG Xin, ZHANG Duoni, LUO Rongrong, XU Wenhao, LI Xun. Effects of Gonadotropin Inhibitory Hormone on Gonadal Reproductive Function and Glucose Metabolism in SD Rats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2239-2249.
[9]	RUAN Yong, CHEN Xiang, DAI Lingang, HUANG Jiajin, AN Dongwei, ZOU Qishun. Correlation Analysis of VNN1 Gene Polymorphism and Lambing Number in F3 Generation Boza Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1384-1392.
[10]	ZHANG Zhishuai, SUI Zhiyuan, ZHANG Jihu, LI Qingjin, XING Feng. Cloning of CNP Gene and Its Tissue Expression Characteristics at Puberty in Dolang Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1402-1412.
[11]	DENG Yihua, WANG Tianjiao, WANG Hongliang, DONG Yimeng, LIU Xin, XING Xiumei. Screening of Specific SNP Sites in Tahe Red Deer Based on Resequencing Technology [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1413-1421.
[12]	SHI Yuzhu, LUO Rutang, CHEN Chao, WU Xu, LI Ang. Cloning, Bioinformatics and Tissue Expression Analysis of GDF9 Genes in Muscovy Duck [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 817-829.
[13]	JIANG Hongzheng, YANG Dezhi, MA Zhonghua, XUN Wenjuan, SHI Liguang, HOU Guanyu. Genome-wide Association Study of Body Size Traits in Danzhou Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 598-607.
[14]	ZHAO Xiaoyu, WU Yingping, LI Haiying, YAO Yingying, LI Jiahui, YAO Yang, LU Qingqing. Construction of Ovarian Transcriptional Profiles and Analysis of Follicle Development-Related Genes in Yili Geese at Various Stages Before and After Egg Laying [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4674-4687.
[15]	ZHOU Ya, ZHANG Heyang, LIU Linling, LI Haodong, ZHENG Junjun, WANG Guiwu. Polymorphism of MSRB3 Gene and Its Association Analysis with Antler Weight Traits in Sika Deer [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4279-4287.