一株解淀粉芽孢杆菌的益生特性及抑菌性研究

doi:10.16431/j.cnki.1671-7236.2020.02.013

Abstract

Abstract: This experiment mainly studied the growth curve,artificial gastroenteric fluid,pH and pig bile salt tolerance of Bacillus amyloliquefaciens DHN04,and its bacteriostatic characteristics and antibiotic sensitivity.The growth curve of the bacteria was measured by growth rate method,the tolerance,acid resistance and bile salt resistance of artificial gastrointestinal fluid were measured by viable count method,the antibacterial activity was measured by Oxford cup method,and the antibiotic sensitivity was measured by drug sensitive test piece.The results showed that Bacillus amyloliquefaciens DHN04 entered the logarithmic growth phase after a slow growth period of 4-6 h,and the stable growth period of the bacteria was 12-24 h.After 3 h in artificial gastric juice and artificial intestinal fluid,the survival rates were 55.55% and 53.33%,respectively.The survival rate of the bacteria was 14.14% at pH 2.0.With the decrease of acidity,the survival rate increased gradually,and the survival rate reached 93.85% at pH 7.0.With the increase of bile salt concentration,the survival rate of Bacillus amyloliquefaciens decreased gradually.The same concentration of bile salt,the survival rate of 24 h was lower than that of 3 h.Bacillus amyloliquefaciens DHN04 had a certain inhibitory effect on Escherichia coli,Salmonella,Staphylococcus aureus and Tetracocci.It was resistant to amoxicillin.In summary,the characteristics of the Bacillus amyloliquefaciens DHN04 were found to be well tolerated by artificial gastrointestinal,bile salts and pH,and had certain inhibitory effects on Escherichia coli,Salmonella and Staphylococcus aureus,and could be activated quickly,which could be used as a potential probiotic strain for livestock and poultry production.

Key words: Bacillus amyloliquefaciens; probiotic characteristics; antibacterial activity; antibiotic sensitivity

CLC Number:

S816

XIA Chaodu, ZHAN Suizhang, AI Qin, LIU Xiaoyan, FANG Binghu. A Study on the Probiotic Characteristics and Antibacterial Activity of a Strain of Bacillus amyloliquefaciens[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 425-432.

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A Study on the Probiotic Characteristics and Antibacterial Activity of a Strain of Bacillus amyloliquefaciens

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