中国美利奴羊IGFBP-3基因内含子4区的PCR-RFLP多态性分析

China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (8): 134-137.

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PCR-RFLP Polymorphism in Intron 4 of IGFBP-3 Gene in Chinese Merino Sheep

SHEN Min¹，WANG Wen-Jun²，YANG Yong-Lin¹，MA Chun-ping³，LIU Shou-ren¹，LI Ning⁴

（1.Key Laboratory for Biotechnology of Sheep Breeding of Xinjiang Production & Construction Corps，Xinjiang Academy of Agricultural Reclamation，Shihezi 832000，China；2.College of Food Science and Engineering，Jiangxi Agricultural University，Nanchang 330045，China；3.Ziniquan Research Institute of Sheep Breeding，Shihezi 832025，China；4.State Key Laboratory for Agrobiotechnology，China Agricultural University，Beijing 100193，China）

Received:1900-01-01 Revised:2010-06-25 Online:2010-08-20 Published:2010-08-20

Abstract

Abstract:

In this study, PCR-RFLP method was established according to a novel SNP site (C218T) within the intron 4 of sheep insulin-like growth factor binding proteins-3 （IGFBP-3） gene. Using this method, 353 Chinese Merino sheeps (Xinjiang Junken type) were tested and illustrated three unique binding genotypes of CC (249 bp), CT (249/216/33 bp) and TT (216/33bp). Frequencies of the genotype CC, CT, TT were 0.52, 0.42, 0.07, respectively, and frequencies of the allele C, T were 0.72, 0.28, respectively. Different genotypes slightly affected several wool traits of Chinese Merino sheeps. Staple length (SL) in individuals of genotype CC was significantly shorter than that in individuals of genotype TT (P＜0.05), follicle density in individuals of genotype CC was significantly higher than that in individuals of genotype CT and TT (P＜0.05).

Key words: insulin-like growth factor binding proteins-3; single nucleotide polymorphism; wool trait; Chinese Merino sheep

CLC Number:

S827.1

SHEN Min;WANG Wen-Jun;YANG Yong-Lin;MA Chun-ping;LIU Shou-ren;LI Ning. PCR-RFLP Polymorphism in Intron 4 of IGFBP-3 Gene in Chinese Merino Sheep [J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(8): 134-137.

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PCR-RFLP Polymorphism in Intron 4 of IGFBP-3 Gene in Chinese Merino Sheep

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