Abstract: (1.College of Animal Science and Technology, Guangxi Univesity, Nanning 530005, China；2. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China；3. Lanzhou Veterinary Research Institute/State Key Laboratory of Veterinary Etiological Biology, Chinese Academy of Agricultural Sciences, Lanzhou 730046,China)〖JZ）〗〖HJ1*2〗〖HJ〗
〖WT5”HZ〗Abstract:〖WT5”BZ〗In order to analyze sequence variation in the partial mitochondrial large subunit ribosomal rRNA gene (rrnL) of Galba pervia from different geographical locations in Guangxi, rrnL genes were amplified from G.pervia isolates obtained from 6 endemic origins. After screening by SSCP technique, representative samples with variable banding patterns were selected for sequencing. DNAStar 5.0 and MEGA 4.0 were used to analyze the sequences subsequently. The results showed that a fragment of the rrnL approximately 500 bp in length was amplified by PCR and a total of 15 variable sites (5.49%) were detected within a 273 bp sequence in 6 endemic origins. There was a notable sequence variation among isolates from same geographical origin in Baise, Nanning and Guilin. Phylogenetic analysis revealed that mitochondrial rrnL was not a good molecular marker for population genetic study of G.pervia to some extent. These results provided novel data for elucidating the genetic variation of G.pervia, the intermediate host of Fasciola in China.

Key words: Galba pervia; mitochondrial rrnL gene; PCR-SSCP; sequence analysis〖