牛源大肠杆菌不同生长阶段的比较蛋白质组学研究

Abstract

Abstract: To investigate the differentially expressed proteins of Escherichia coli from mastitic cow during growth phase，two-dimensional electrophoresis coupled with mass spectrometry method were used to separate and identify the changes of proteins in the lag phase，exponential phase and stationary phase of Escherichia coli.The results showed that some protein compositions of Escherichia coli were changed in lag phase，exponential phase and stationary phase.Relative abundance of outer membrane protein W and phosphoenolpyruvate carboxykinase in Escherichia coli were increased，while periplasmic protein，outer membrane protein Ⅱ and phosphotransacetylase were decreased in the lag phase comparative with exponential phase and stationary phase.Results of this study help provide the relationships between proteins expression and the growth of this pathogen，and were used to control and protect the host against the pathogen.

Key words: two-dimensional electrophoresis; growth phase; proteomic; Escherichia coli

ZHAO Xiao-wei，YANG Yong-xin，CHENG Guang-long，HUANG Dong-wei，ZHAO Hui-ling. Comparative Proteomic Analysis of Escherichia coli Protein during Growth Phase[J]. .

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Comparative Proteomic Analysis of Escherichia coli Protein during Growth Phase

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