不同羊毛纤维直径细毛羊皮肤组织差异表达蛋白质研究

doi:10.16431/j.cnki.1671-7236.2016.04.005

Abstract

Abstract: The purpose of this paper was to study the differentially expressed proteins of hair follicles in skin tissue with different fiber diameter of Fine-wool sheep, and discussed the function of proteins related to wool fineness.The differentially expressed protein maps of Fine-wool sheep skin hair follicle were established by two-dimensional gel electrophoresis, and the differentially expressed protein spots were detected and analyzed by the ImageMaster 2D Platinum software, Fine-wool sheep skin hair follicle specific proteins were classified and functional identified by mass spectrum identification technology and database matching method.The results showed that 94 different proteins were successfully identified in Fine-wool sheep with different fiber diameter, including 73 different proteins between superfine wool sheep and Fine-wool sheep at the same age, and 21 different proteins of superfine wool sheep at the different age.According to the functional properties got eight kind of functional proteins, keratin, molecular chaperone protein, cytoskeleton protein, 14-3-3 protein, protease, myosin, serum albumin, other protein, respectively.These results indicated that the internal relation between the differentially expressed protein and the wool fiber diameter was analyzed by the bioinformatics knowledge, which would provide effective genetic resources information directly for high quality breeding of Fine-wool sheep, and had important practical significance for improving the quality of the wool.

Key words: Fine-wool sheep; two-dimensional gel electrophoresis; proteomics; differentially expressed proteins

CLC Number:

TQ93

FU Xue-feng, YANG Han-yulu, SHI Gang, XU Xin-ming, ZHANG En-ping, DI Jiang, ZHANG Yan-hua, HUANG Xi-xia, TIAN Ke-chuan. Proteome Array on Differentially Expressed Proteins of Skin Tissue in Fine-wool Sheep with Different Fiber Diameter[J]. , 2016, 43(4): 879-891.

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Proteome Array on Differentially Expressed Proteins of Skin Tissue in Fine-wool Sheep with Different Fiber Diameter

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