细毛羊皮肤组织中毛囊蛋白质2-DE图谱的建立与初步分析

doi:10.16431/j.cnki.1671-7236.2015.01.019

Abstract

Abstract: In use of hair follicles in skin tissue of fine-wool sheep with different fiber diameters as experimental material, and different extraction methods, IPG strips with different pH and different sample volume as experimental methods, this paper was aimed to explore two-dimensional electrophoresis system applied to skin tissue of the fine-wool sheep and establish 2-DE gel map of skin tissue in fine-wool sheep.Gel maps using ImageMaster 2D Platinum software automatically detected protein points to compare different protein fiber diameter of fine-wool sheep differences.The results showed that the TCA/acetone extraction method was most suited to fine-wool sheep hair follicle tissue extraction of total protein.Choose to 18 cm, pH 4 to 7 linear IPG strip, we got better quality two-dimensional gel electrophoresis, got 35 protein variations among the different cantons for the follow-up work fine-wool sheep with high quality of the study of proteomics.

Key words: fine-wool sheep; two-dimensional electrophoresis; hair follicles; proteomics

CLC Number:

TQ93

YANG Jie, FU Xue-feng, HUANG Xi-xia, XU Xin-ming, SHI Gang, ZHAN Zhen-hong, ZHANG En-ping, TIAN Ke-chuan. Establishment and Preliminary Studies on the Skin Tissue Hair Follicles Proteins of 2-DE Map Building in Fine-wool Sheep[J]. , 2015, 42(1): 124-130.

References

[1] Allain D,Lantier I,Elsen J M,et al.A design aiming at detecting QTL controlling wool traits and other traits in the INRA401 sheep line[J].Genetics,1998,5:203-207.
[2] Ponz R,Moreno C,Allain D,et al.Assessement of genetic variation explained by markers for wool traits in sheep via a segment mapping approach[J].Mamm Genome,2001,12:569-572.
[3] Bidinost F.Quantitative trait loci related to merino sheep wool quality[A].The 8th World Congress on Genetics Applied to Livestock Production[C].2006.
[4] 马玉萍.绵羊主要经济性状的微卫星标记研究[D].石河子:石河子大学,2003.
[5] 徐新明.超细型细毛羊遗传多样性及羊毛性状的微卫星分析[D].乌鲁木齐:新疆农业大学,2007.
[6] 于国庆,徐铁山,孙陶,等.绵羊血浆蛋白质组2-DE图谱的初步建立分析与质谱检测[J].现代畜牧兽医,2013(6):54-56.
[7] 裴艳涛,赵宝华,孙继国.绵羊肺炎支原体蛋白质组双向电泳图谱的构建[J].安徽农业科学,2007,35(11): 3276-3277.
[8] Margaret G.Observations on the abomasal proteome during Teladorsagia circumcincta infection in sheep[D].Edinburgh:The University of Edinburgh,2010.
[9] Pisanu S,Ghisaura S,Pagnozzi D,et al.Charactcrization of sheep milk fat globule proteins by two-dimensional polyacryl-amide gel electrophoresis/mass spectrometry and generation of a reference map[J].International Dairy Journal,2012,24(2):78-86.
[10] 松杰,董扬,孟丽云,等.内蒙古绒山羊皮肤蛋白质双向电泳条件优化及图谱建立[J].中国畜牧兽医,2012,39(7):11-14.
[11] Carpentier S C,Witters E,Laukens K,et al.Preparation of protein extracts from recalcitrant plant tissues: an evaluation of different methods for two-dimensional gel electrophoresis analysis[J].Proteomics,2005,5(10):2497-2507.
[12] Pasquali C,Fialka I,Huber L A.Preparative two-dimensional gel electrophoresis of membrane proteins [J].Electrophoresis,1997,18(14):2573-2581.
[13] 林雍峰.绵羊卵母细胞差异蛋白质组学研究[D].兰州:甘肃农业大学,2011.
[14] 张丽军,谢锦云,张健,等.2DE中IPG胶条转移到SDS-PAGE中的技术改进[J].生命科学研究,2004,8(3):225-230.
[15] 郭忠军.干旱胁迫下棉花蛋白质组双向电泳体系构建与差异表达蛋白功能分析[D].乌鲁木齐:新疆农业大学,2012.
[16] 张颖,杨舸.蛋白质组学研究的有效工具——双向电泳鉴定技术[J].重庆文理学院学报(自然科学版),2010,29(5):66-68.
[17] 钟伯雄,颜海燕,沈飞英,等.家蚕蛋白质双向电泳的样品制备方法[J].蚕业科学,2003,29(4): 427-432.
[18] 朱雅卿,韩剑众,王彦波.猪骨骼肌双向电泳样品制备及等电聚焦方法研究[J].中国食品学报,2009,9(3): 99-104.
[19] 徐永杰,熊远.猪肌肉组织双向电泳分离条件的建立及常见问题分析[J].农业生物技术学报,2012,20(1): 105-112.
[20] 高丽霞,刘志红,张燕军,等.绒山羊皮肤蛋白双向电泳条件的优化与常见问题分析[J].农业生物技术学报,2014,22(2): 249-256.
[21] 胡惠忠,付强,卢晟盛,等.猪卵母细胞蛋白质组双向电泳体系的建立及初步分析[J].动物学杂志,2008,43(4):67-71.
[22] 王一鸣,花宝光,王有年,等.桃果实蛋白质双向电泳影响因素的研究[J].园艺学报,2007,6:244-249.
[23] 马洪雨,王占奎,俞阗,等.适用于黄麻根部蛋白质组学分析的双向电泳技术[J].西北植物学报,2010,30(1):195-202.
[24] 张丽,罗海波,姜丽,等.果实成熟衰老过程中蛋白质组学研究进展[J].植物生理学报,2011,47(9):861-871.

Establishment and Preliminary Studies on the Skin Tissue Hair Follicles Proteins of 2-DE Map Building in Fine-wool Sheep

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