番鸭脂肪酸合成酶基因的克隆及填饲对其mRNA水平的影响

Abstract

Abstract: The Muscovy duck were used to clone the sequence of fatty acid synthase (FAS) gene by RT-PCR，the sequence was analyzed by related softwares and on-line tools, the tissue-specific expression and the effect of overfeeding on FAS gene mRNA level were performed by Real-time PCR. The results showed that the cloned sequence was 1122 bp and encoded 374 amino acids. The nucleotide homology of Muscovy duck FAS was high similar with other avain species from 92% to 99%. The tissue-specific expression result showed that the mRNA expression level of Muscovy duck FAS gene was the higher in liver and adipose tissues and was not detected or little in other 10 tissues. It revealed that Muscovy duck FAS gene was tissue specific.The mRNA expression of Muscovy duck FAS gene in liver was significantly increased after overfeeding （P<0.05）.The results indicated that FAS gene could play an important role in duck liver steatosis.

Key words: Muscovy duck; fatty acid synthase; gene clone; overfeeding

ZHANG Yi-hui，ZHANG Rui，WANG Jian，NIU Jun-wei，SUN Xiao-xian，GONG Dao-qing. Cloning of Muscovy Duck Fatty Acid Synthase Gene and the Effect of Overfeeding on its mRNA Level[J]. China Animal Husbandry & Veterinary Medicine.

References

1 刘振春，郭炫，杨童奥，等.填饲对鹅肥肝脂肪酸合成酶基因mRNA表达的影响［J］.生物工程，2012，33（5)：165～169.
2 单体中，汪以真，刘建新，等.不同日龄猪腹脂中脂肪酸合成酶（FAS)基因表达规律的研究［J］.畜牧兽医学报，2006，37（7）：662～666.
3 彭祥伟，范守成，邢豫川，等.鹅肥肝的生产及其FAS活性测定［J］.中国家禽，2005，9（1）：48～51.
4 舒常平，王宝维，李桢，等.填饲鹅肝脏组织中脂肪酸沉积与FAS基因mRNA的表达丰度［J］.中国农业科学，2012，45（10）：2002～2011.
5 熊文中，杨风，周安国.猪重组生长激素对不同杂交肥育猪脂肪代谢调控的研究［J］.畜牧兽医学报，2001，32（1）：1～4.
6 Chirala S S，Kasturi R，Pazirandeh M，et al.A novel cDNA extension procedure.Isolation of chicken fatty acid synthase cDNA clones［J］.J Biol Chem，1989，264（7）：3750～3757.
7 Fournier E，Peresson R，Guy G，et al. Relationships between storage and secretion of hepatic lipids in two breeds of geese with different susceptibility to liver steatosis［J］.Poultry Science，1997，76（4）：599～607.
8 Hermier D，Saadoun A，Salichon M R，et al. Plasma lipoproteins and liver lipids in two breeds of geese with different susceptibility to hepatic steatosis：Changes induced by development and force-feeding［J］.Lipids，1991，26（5）：331～339.
9 Holzer K P，Liu W，Hammes G G. Molecular cloning and sequencing of chicken liver fatty acid synthase cDNA［J］.PNAS，1989，86(12):4387～4391.
10 Kameda K，Goodridge A G. Isolation and partial characterization of the gene for goose fatty acid synthase［J］.J Biol Chem，1991，266（1）：419～426.
11 Leveille G A. Glycogen metabolism in meal-fed rats and chicks and time sequence of lipogenic and enzymeatic adaptive changes［J］.J Nutr，1967，90：449～460.
12 Mourot J，Guy G，Lagarrigue S，et al. Role of hepatic lipogenesis in the susceptibility to fatty liver in the goose (Anser anser)［J］.Comp Biochem Physiol B Biochem Mol Biol，2000，126（1）：81～87.
13 Nathalie L E，Catherine E L，Rohan S，et,al. Characterization of the chicken fatty acid synthase gene 5′part and promoter region［J］.Eur J Biochem，1996，240（2）：323～330.
14 Romsos D R，Allee G L，Leveille G A. In vivo cholesterol and fatty acid synthesis in the pig intestine［J］.Exp Bioi Med，1971，137：570～573.
15 Roy R，Taourit S，Zaragoza P，et al. Genomic structure and alternative transcript of bovine fatty acid synthase gene(FASN): Comparative analysis of the FASN gene between monogastric and ruminant species［J］.Cytogenet Genome Res，2005，111（1）：65～73.
16 Semenkovich C F. Regulation of fatty acid synthase(FAS)［J］.Progress in Lipid Research，1997，6（1）：43～53.
17 Smith S，Witkowski A，Joshi A K. Structural and functional organization of the animal fatty acid synthase［J］.Progress in Lipid Research，2003，42（4）：289～317.

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Cloning of Muscovy Duck Fatty Acid Synthase Gene and the Effect of Overfeeding on its mRNA Level

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