番鸭脂肪酸合成酶基因的克隆及填饲对其mRNA水平的影响

Abstract

Abstract: The Muscovy duck were used to clone the sequence of fatty acid synthase (FAS) gene by RT-PCR，the sequence was analyzed by related softwares and on-line tools, the tissue-specific expression and the effect of overfeeding on FAS gene mRNA level were performed by Real-time PCR. The results showed that the cloned sequence was 1122 bp and encoded 374 amino acids. The nucleotide homology of Muscovy duck FAS was high similar with other avain species from 92% to 99%. The tissue-specific expression result showed that the mRNA expression level of Muscovy duck FAS gene was the higher in liver and adipose tissues and was not detected or little in other 10 tissues. It revealed that Muscovy duck FAS gene was tissue specific.The mRNA expression of Muscovy duck FAS gene in liver was significantly increased after overfeeding （P<0.05）.The results indicated that FAS gene could play an important role in duck liver steatosis.

Key words: Muscovy duck; fatty acid synthase; gene clone; overfeeding

ZHANG Yi-hui，ZHANG Rui，WANG Jian，NIU Jun-wei，SUN Xiao-xian，GONG Dao-qing. Cloning of Muscovy Duck Fatty Acid Synthase Gene and the Effect of Overfeeding on its mRNA Level[J]. .

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Cloning of Muscovy Duck Fatty Acid Synthase Gene and the Effect of Overfeeding on its mRNA Level

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