鸡传染性支气管炎病毒广西株M基因的克隆与序列分析

doi:10.16431/j.cnki.1671-7236.2016.02.013

Abstract

Abstract: According to the M gene nucleotide sequence of avian infectious bronchitis virus (IBV) published in GenBank,one pair of primers were designed,the M gene fragments of IBV isolated from Guangxi province were amplified by PCR.Then the amplified fragments were cloned into pMD18-T vector and the positive recombinant plasmids were sequenced.The results showed that M gene from all of the IBV isolates consisted of 678 bp,coding for 225 amino acids.Two glycosylated sites were located nearby the N-terminal,three transmembrane domains were located in the 23 to 98 peptide region.Variations within the hydrophilicity region were easier than that in the hydrophobicity region.Compared with that of other published IBV strains,the homologies of nucleotide and amino acid sequences of the isolates were 83.6% to 92.5% and 82.7% to 95.1%,respectively.The phylogenetic tree analysis showed that it was closely related to SAIB20 and LX4,and clustered into one group;But it belonged to different branches with other reference strains,and had a distant relationship.These results suggested that the isolate was a new variant of IBV.

Key words: avian infectious bronchitis virus; M gene; gene clone; sequence analysis

CLC Number:

QU Su-jie, SHI Kai-chuang, ZOU Lian-bin, HU Jie, MO Sheng-lan, YIN Yan-wen, ZHANG Bu-xian, LI Jun, LIANG Yuan. Cloning and Sequence Analysis of M Gene of Avian Infectious Bronchitis Virus Guangxi Strain[J]. , 2016, 43(2): 377-382.

References

[1] 殷震,刘景华.动物病毒学[M].第2版.北京:科学技术出版社,1997.
[2] Saif L J.Animal coronaviruses:What can they teach us about the severe acute respiratory syndrome[J]. Rev Sci Technol,2004,23:643-660.
[3] 余娟,刘兴友,王玲丽.鸡传染性支气管炎病毒致病机理的研究进展[J].中国畜牧兽医,2011,38(12):160-162.
[4] Ignjatovic J,Ashton D F,Reece R,et al.Pathogenicity of Australian strains of avian infectious bronchitis virus[J].Comp Pathol,2002,126:115-123.
[5] Jia W,Karaca K,Parrish C R,et al.A novel variant of avian infectious bronchitis virus resulting from recombination among three different strains[J].Arch Virol,1995,40(2):259-271.
[6] 卡尔尼卡.禽病学[M].第9版.北京:北京农业大学出版社,1991.
[7] Cavanagh D.Coronavirus IBV gly copolypeptides:Size of their polypeptide moieties and nature of their liga charides[J].J Gen Virol,1983,64:1187-1191.
[8] Lim K P,Liu D X.The missing link in coronavirus assembly Retenion of the avian coronavirus Infectious bronchitis virus envelope protein in the pre-Gogil compartments and physical interaction between the envelop and membrane protein[J].J Bio Chem,2001,276(20):17515-17523.
[9] Gen M L,Li S L,Li J Y,et al.Assembly and immunogenicity of coronavirus-like particles carrying infectious bronchitis virus M and S proteins[J].Vaccine,2013,31(47):5524-5530.
[10] 刘胜旺,贺秀瑗,孔宪刚,等.鸡传染性支气管炎病毒中国地方分离株 M 基因的分子特征[J].中国预防兽医学报,2001,23(6):404-407.
[11] 贺秀媛,张淑霞,张君涛,等.鸡传染性支气管炎病毒 W 株膜蛋白基因的分子特征[J].西北农林科技大学学报:自然科学版,2009,37(9):27-33.
[12] Binns M M,Boursnell M E,Tomley F M,et al.Nucleotide sequence encoding the membrane protein of the IBV strain 6/82[J].Nuleic Acids Res,1986,14(13):55-58.
[13] Stern D,Sefton B.Coronavirus proteins:Structure and function of the oligosaccharides of the avian infectious bronchitis virus glycoproteins[J].J Virol,1982,44(3):804-812.
[14] Zong X H,Chu Y S,Bao L Y,et al.A 15-year analysis of molecular epidemiology of avian infectious bronchitis coronavirus in China[J].Infect Genet Evol,2011,11(1):190-200.

[1]	ZHANG Mingliang, GENG Yachun, LIAN Kaiqi, MA Lei, CHANG Ying, WANG Shuangshan, ZHANG Fuliang. Cloning,Bioinformatics Analysis and Truncated Fragment Expression for Truncated M Gene of Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1479-1487.
[2]	SONG Chun, WANG Bolin, LI Mei, WEN Ming, WANG Kaigong, CHEN Changxiu, CHENG Zhentao. Isolation and Identification of Mycoplasma synoviae Guizhou Strain and Sequence Analysis of VlhA Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1516-1523.
[3]	WEI Yunfang, LI Feixiang, Xing Yun, Huang Qingguo, LI Judong, WAN Jiusheng, CHEN Fangliang. Study on Cloning, Bioinformatics Analysis and Spatio-temporal Expression of IGF2 Gene in Kunming Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 866-875.
[4]	HE Xianming, REN Guangcai, YE Junxian, LAN Hong, LIU Yufu, XIONG Ting, YANG Zekun, XU Ting, CHEN Ruiai. Isolation and Identification of a Strain of Infectious Bursal Disease Virus and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1005-1014.
[5]	CAI Jie, WU Jiayi, PAN Yuanxia, HU Gaowei, ZHANG Linzhou. Isolation, Identification and Drug Resistance Analysis of Chryseobacterium sp. from Pseudosciaena crocea [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1135-1143.
[6]	HU Xiaobing, LIN Biaosheng, WANG Zhenwei, ZHENG Lijing. Isolation, Identification and Genomic Sequence Analysis of a Strain of Clostridium butyricum from Pigs Utilizing Lactic Acid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 569-578.
[7]	KOU Meiling, MIAO Haisheng, LI Le, XIE Jiarui, LI Huachun, SONG Jianling. Isolation and Identification of Chuzan Virus from Palyam Serogroup Virus in Yunnan Border Area [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4401-4409.
[8]	TAN Bin, WANG Chao, WANG Yan, ZHANG Shuqin. Isolation and Identification of Parvovirus from Palm Civets and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4012-4018.
[9]	WANG Zhiyan, SUN Yujie, XU Xin, HUANG Baicheng, TIAN Kegong. Isolation,Identification and Pathogenicity of Porcine Deltacoronavirus Strain HeN10 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2982-2989.
[10]	LI Nan, HE Yuwen, ZUO Yuanyuan, MENG Jinxin, XU Tiangang, WANG Jinglin. Analysis of Molecular Biological Characteristics of Structural Genes of Getah Virus (SZC30) Isolated from Midge [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1149-1160.
[11]	ZHANG Qi, CHEN Haotian, SHAN Jiabao, CHANG Rui, ZHANG Xue, WANG Yao, WANG Junwei, MA Bo. Cloning and Sequence Analysis of NS1 and VP1 Genes of Gosling Plague Virus from Jilin District [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1161-1169.
[12]	GUO Mengjiao, LI Mingtao, TAN Huihui, ZHANG Chengcheng, ZHANG Xiaorong, WU Yantao. Study on Cloning and Expression Characteristic of NLRC3 Gene in Rabbit [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 3940-3949.
[13]	WANG Zhengfan, ZHU Lisai, LI Fei, XIANG Rui, LI Yiyun, YING Biyun, WANG Guiping, BAI Aiquan, JIA Aiqing. Pathogenicity and Whole Genome Sequence Analysis of Porcine Deltacoronavirus CH7328 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4143-4153.
[14]	YIN Dejing, ZHANG Yundan, YUE Jun, LI Qiaobin, HE Ling, LI Tao, WANG Kaigong, CHENG Zhentao. Whole Genomic Sequencing Analysis of Fowl Adenovirus Serotype 4 from Guizhou [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 44-54.
[15]	PAN Pengcheng, WEN Binhua, XIE Wan, JIANG Changjin, JIAO Di, CHEN Baojian, GUAN Zhihui, XIE Bingkun. Sequence Analysis,Eukaryotic Expression Vector Construction and Tissue Expression Analysis of PDK4 Gene in Luchuan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2337-2347.

Cloning and Sequence Analysis of M Gene of Avian Infectious Bronchitis Virus Guangxi Strain

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments