鸡传染性支气管炎病毒广西株N、M基因的克隆与序列分析

doi:10.16431/j.cnki.1671-7236.2015.12.002

Abstract

Abstract: To investigate genetic variation of avian infectious bronchitis virus (IBV) in Guangxi province,one strain of IBV was isolated from chicken.Two pairs of primers for amplifying the N and M genes of IBV were designed according to the sequences in GenBank.The N and M genes of the strain were amplified by RT-PCR,and they were proved to be the N and M genes of IBV by cloning,sequencing and compared with reference IBV strains published in GenBank.The results showed that the N gene from the IBV isolate consisted of 1 230 bp,coding 409 amino acids.The M gene from the IBV isolate consisted of 678 bp,coding 225 amino acids.The sequence analysis of N gene showed that it shared 87.2% to 93.3% nucleotide homologies and 90.0% to 94.4% deduced amino acid sequence homologies with IBV strains from GenBank.The M gene sequence analysis showed that it shared 83.6% to 91.0% nucleotide homologies and 82.7% to 92.9% deduced amino acid sequence homologies.The phylogenetic tree analysis showed that it was closely related to BJ and LX4 strains,and were clustered into one group;But with the distant relatives from other strains of IBV.These results suggested that the isolate was a new variant of IBV.

Key words: avian infectious bronchitis virus; N gene; M gene; gene clone; sequence analysis

CLC Number:

S852.65

QU Su-jie, SHI Kai-chuang, SU Yan-qiong, HU Jie, MO Sheng-lan, ZHANG Bu-xian, LI Jun, ZOU Lian-bin. Cloning and Sequence Analysis of N and M Genes of Avian Infectious Bronchitis Virus Guangxi Strain[J]. , 2015, 42(12): 3119-3125.

References

[1] 殷震,刘景华.动物病毒学[M].第2 版.北京:科学技术出版社,1997.
[2] Saif L J.Animal coronaviruses:What can they teach us about the severe acute respiratory syndrome[J].Rev Sci Technol,2004,23:643-660.
[3] 王延树,尤永君,李建丽,等.鸡传染性支气管炎病毒 BJ0903 株的分离鉴定[J].中国畜牧兽医,2010,37(2):156-158.
[4] Ignjatovic J,Ashton D F,Reece R,et al.Pathogenicity of Australian strains of avian infectious bronchitis virus[J].J Comp Pathol,2002,126(2-3):115-123.
[5] Wang L,Junker D,Collisson E.Evidence of natural recombination within the S1 gene of infectious bronchitis virus[J].Virology,1993,192(2):710-716.
[6] Jia W,Karaca K,Parrish C R,et al.A novel variant of avian infectious bronchitis virus resulting from recombination among three different strains[J].Arch Virol,1995,140(2):259-271.
[7] 卡尔尼卡著.高福,刘文军主译.禽病学[J].第9版.北京:北京农业大学出版社,1991.
[8] 何长生,魏建忠,刘胜旺,等.IBV AH1-99分离株N基因的克隆及序列分析[J].动物医学进展,2008,29(1):5-9.
[9] Lim K P,Liu D X.The missing link in coronavirus assembly.Retention of the avian coronavirus infectious bronchitis virus envelope protein in the pre-Golgi compartments and physical interaction between the envelope and membrane proteins[J].J Bio Chem,2001,276(20):17515-17523.
[10] Gen M L,Li S L,Li J Y,et al.Assembly and immunogenicity of coronavirus-like particles carrying infectious bronchitis virus M and S proteins[J].Vaccine,2013,31(47):5524-5530.
[11] Cavanagh D,Davis P J.Evolution of avian coronavirus IBV:Sequence of the matrix glycoprotein gene and intergenic region of several serotypes[J].J Gen Virol,1988,69:621-629.
[12] Zong X H,Chu Y S,Bao L Y,et al.A 15-year analysis of molecular epidemiology of avian infectious bronchitis coronavirus in China[J].Infect Genet Evol,2011,11(1):190-200.

[1]	SUN Shouhu, CHEN Zhifei, GUO Zhixuan, YU Ting, WANG Xiuwu, LI Songbei, WEI Wenkang, HE Dongsheng. Study on Codon Usage Bias of N gene of Three Porcine Coronaviruses and Their Host Adaptive Mechanism [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2403-2413.
[2]	ZHANG Mingliang, GENG Yachun, LIAN Kaiqi, MA Lei, CHANG Ying, WANG Shuangshan, ZHANG Fuliang. Cloning,Bioinformatics Analysis and Truncated Fragment Expression for Truncated M Gene of Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1479-1487.
[3]	SONG Chun, WANG Bolin, LI Mei, WEN Ming, WANG Kaigong, CHEN Changxiu, CHENG Zhentao. Isolation and Identification of Mycoplasma synoviae Guizhou Strain and Sequence Analysis of VlhA Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1516-1523.
[4]	WEI Yunfang, LI Feixiang, Xing Yun, Huang Qingguo, LI Judong, WAN Jiusheng, CHEN Fangliang. Study on Cloning, Bioinformatics Analysis and Spatio-temporal Expression of IGF2 Gene in Kunming Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 866-875.
[5]	HE Xianming, REN Guangcai, YE Junxian, LAN Hong, LIU Yufu, XIONG Ting, YANG Zekun, XU Ting, CHEN Ruiai. Isolation and Identification of a Strain of Infectious Bursal Disease Virus and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1005-1014.
[6]	CAI Jie, WU Jiayi, PAN Yuanxia, HU Gaowei, ZHANG Linzhou. Isolation, Identification and Drug Resistance Analysis of Chryseobacterium sp. from Pseudosciaena crocea [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1135-1143.
[7]	HU Xiaobing, LIN Biaosheng, WANG Zhenwei, ZHENG Lijing. Isolation, Identification and Genomic Sequence Analysis of a Strain of Clostridium butyricum from Pigs Utilizing Lactic Acid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 569-578.
[8]	KOU Meiling, MIAO Haisheng, LI Le, XIE Jiarui, LI Huachun, SONG Jianling. Isolation and Identification of Chuzan Virus from Palyam Serogroup Virus in Yunnan Border Area [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4401-4409.
[9]	TAN Bin, WANG Chao, WANG Yan, ZHANG Shuqin. Isolation and Identification of Parvovirus from Palm Civets and Sequence Analysis of VP2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4012-4018.
[10]	WANG Zhiyan, SUN Yujie, XU Xin, HUANG Baicheng, TIAN Kegong. Isolation,Identification and Pathogenicity of Porcine Deltacoronavirus Strain HeN10 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2982-2989.
[11]	LI Nan, HE Yuwen, ZUO Yuanyuan, MENG Jinxin, XU Tiangang, WANG Jinglin. Analysis of Molecular Biological Characteristics of Structural Genes of Getah Virus (SZC30) Isolated from Midge [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1149-1160.
[12]	ZHANG Qi, CHEN Haotian, SHAN Jiabao, CHANG Rui, ZHANG Xue, WANG Yao, WANG Junwei, MA Bo. Cloning and Sequence Analysis of NS1 and VP1 Genes of Gosling Plague Virus from Jilin District [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1161-1169.
[13]	GUO Mengjiao, LI Mingtao, TAN Huihui, ZHANG Chengcheng, ZHANG Xiaorong, WU Yantao. Study on Cloning and Expression Characteristic of NLRC3 Gene in Rabbit [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 3940-3949.
[14]	WANG Zhengfan, ZHU Lisai, LI Fei, XIANG Rui, LI Yiyun, YING Biyun, WANG Guiping, BAI Aiquan, JIA Aiqing. Pathogenicity and Whole Genome Sequence Analysis of Porcine Deltacoronavirus CH7328 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4143-4153.
[15]	YIN Dejing, ZHANG Yundan, YUE Jun, LI Qiaobin, HE Ling, LI Tao, WANG Kaigong, CHENG Zhentao. Whole Genomic Sequencing Analysis of Fowl Adenovirus Serotype 4 from Guizhou [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 44-54.

Cloning and Sequence Analysis of N and M Genes of Avian Infectious Bronchitis Virus Guangxi Strain

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments