番鸭细小病毒浙江分离株VP基因的克隆与序列分析

doi:10.16431/j.cnki.1671-7236.2015.10.013

Abstract

Abstract: In order to enrich the biological characteristics of VP gene from Muscovy duck parvovirus Zhejiang isolate (MDPV-ZJ),the target VP gene fragments were amplified by PCR method with specific primers,then the obtained PCR products were cloned and sequenced.The bioinformatics analysis of VP gene of MDPV-ZJ was conducted.The results revealed that MDPV-ZJ VP gene was 2 199 bp in length,coding an open reading frame (ORF) with 732 amino acids.The molecular weight,theoretical isoelectric point,instability index and grand average of hydropathicity of MDPV-ZJ VP gene were 81.32 ku,6.59,37.49 and －0.667,respectively,and with no signal peptide.The genetic evdution ary tree based on the VP gene showed that the MDPV isoaltes contains two groups:The typical MDPV and recombinant MDPV; Also,the typical MDPV could be divided into three branches:Taiwanese branch,Maniland China branch and Euro branch,which existed obvious regional genetic evolution relationship.In this assay MDPV-ZJ was belonged to MDPV Maniland China branch.

Key words: Muscovy duck parvovirus; VP gene; clone; sequence analysis

CLC Number:

WAN Chun-he, CHEN Hong-mei, FU Qiu-ling, SHI Shao-hua, FU Guang-hua, CHENG Long-fei, HUANG Yu, HU Kai-hui. Cloning and Sequence Analysis of VP gene of Muscovy Duck Parvovirus Zhejiang Isolate[J]. , 2015, 42(10): 2600-2605.

References

[1] 林世棠,郁晓岚,陈炳钿,等.一种新的雏番鸭病毒性传染病的诊断[J].中国畜禽传染病,1991,2:27-28.
[2] 程由铨,林天龙,胡奇林,等.雏番鸭细小病毒病的病毒分离和鉴定[J].病毒学报,1993,9(3):39-46.
[3] 程由铨,胡奇林,李怡英,等.番鸭细小病毒弱毒株的选育及其生物学特性[J].中国兽医学报,1996,16(2):118-121.
[4] 陈少莺,胡奇林,程晓霞,等.雏番鸭细小病毒病显微和超微结构研究[J].中国预防兽医学报,2001,23(2):25-28.
[5] Zadori Z,Stefancsik R,Rauch T,et al.Analysis of the complete nucleotide sequences of goose and muscovy duck parvoviruses indicates common ancestral origin with adeno-associated virus 2[J].Virology,1995,212(2):562-573.
[6] 张云,耿宏伟,郭东春,等.鹅和番鸭细小病毒全基因克隆与序列分析[J].中国预防兽医学报,2008,30(6):415-419.
[7] 万春和,潘异哲,陈红梅,等.区别鹅细小病毒和番鸭细小病毒的PCR-RFLP方法的建立[J].中国畜牧兽医,2014,41(9):56-59.
[8] Le Gall-Recule G,Jestin V,Chagnaud P,et al.Expression of muscovy duck parvovirus capsid proteins (VP2 and VP3) in a baculovirus expression system and demonstration of immunity induced by the recombinant proteins[J].J Gen Virol,1996,77(9):2159-2163.
[9] Tsai H,Tseng C,Chang P,et al.Genetic variation of viral protein 1 genes of field strains of waterfowl parvoviruses and their attenuated derivatives[J].Avian Dis,2004,48(3):512-521.
[10] Zhu Y,Zhou Z,Huang Y,et al.Identification of a recombinant Muscovy duck parvovirus (MDPV) in Shanghai,China[J].Vet Microbiol,2014,174(3-4):560-564.
[11] Zhao H,Xie Z,Xie L,et al.Molecular characterization of the full muscovy duck parvovirus,isolated in Guangxi,China[J].Genome Announc,2014,2(6):e01249-14.
[12] Zhang Y,Li Y,Liu M,et al.Development and evaluation of a VP3-ELISA for the detection of goose and Muscovy duck parvovirus antibodies[J].J Virol Methods,2010,163(2):405-409.
[13] Takehara K,Nakata T,Takizawa K,et al.Expression of goose parvovirus VP1 capsid protein by a baculovirus expression system and establishment of fluorescent antibody test to diagnose goose parvovirus infection[J].Arch Virol,1999,144(8):1639-1645.
[14] Ohshima T,Mochizuki M.Evidence for recombination between feline panleukopenia virus and canine parvovirus type 2[J].J Vet Med Sci,2009,71(4):403-408.
[15] Wang J,Cheng S,Yi L,et al.Evidence for natural recombination between mink enteritis virus and canine parvovirus[J].Virol J,2012,9:252.
[16] Guo L,Yang S L,Chen S J,et al.Identification of canine parvovirus with the Q370R point mutation in the VP2 gene from a giant panda (Ailuropoda melanoleuca)[J].Virol J,2013,10:163.
[17] Khamrin P,Okitsu S,Ushijima H,et al.Complete genome sequence analysis of novel human bocavirus reveals genetic recombination between human bocavirus 2 and human bocavirus 4[J].Infect Genet Evol,2013,17:132-136.
[18] Poonia B,Dunn PA,Lu H,et al.Isolation and molecular characterization of a new Muscovy duck parvovirus from Muscovy ducks in the USA[J].Avian Pathol,2006,35(6):435-441.
[19] Palya V,Zolnai A,Benyeda Z,et al.Short beak and dwarfism syndrome of mule duck is caused by a distinct lineage of goose parvovirus[J].Avian Pathol,2009,38(2):175-180.
[20] 王劭,朱小丽,林峰强,等.番鸭源小鹅瘟病毒PT株VP基因的克隆与序列分析[J].农业生物技术学报,2012,20(1):79-84.
[21] Wang S,Cheng X,Chen S,et al.Genetic characterization of a potentially novel goose parvovirus circulating in Muscovy duck flocks in Fujian province,China[J].J Vet Med Sci,2013,75(8):1127-1130.

Cloning and Sequence Analysis of VP gene of Muscovy Duck Parvovirus Zhejiang Isolate

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