猪传染性胸膜肺炎放线杆菌PCR诊断方法的建立与应用

Abstract

Abstract: According to the APP included in GenBank serum surface antigen of D15 type 1 to 14 and 16S rRNA gene sequences were designed two pairs of primers, namely amplified two nucleotide fragments of 637, 431 bp, and built PCR detection method of APP. The results indicated that type-specific serum 1, 3, 5a, 8, 10 could amplify two expected fragments and Escherichia coli, Pasteurella, Salmonella, Streptococci and Staphylococci, and they were negative. Sensitivity checked out test results showed that the lowest concentration was 50/μL. 5 strains of clinical isolates strain suspected APP by PCR ,the results showed that in four of them were positive, one was negative. The specificity of PCR detection method of the experimental establishment was strong specificity, high sensitivity.

Key words: porcine contagious pleuropneumonia; D15 gene; 16S rRNA; polymerase chain reaction(PCR)

CLC Number:

S855.1⁺2

ZHANG Jian-xin, ZHU Yan-kun, MA Jin-you, ZHAO Jie, YU Yan, HAN Ke-fang. Establishment and Application of PCR Method for Diagnosing Actinobacillus pleuropneumoniae[J]. , 2013, 40(4): 69-72.

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Establishment and Application of PCR Method for Diagnosing Actinobacillus pleuropneumoniae

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