洛阳某猪场猪传染性胸膜肺炎放线杆菌的分离鉴定

doi:10.16431/j.cnki.1671-7236.2020.04.031

Abstract

Abstract: In order to determine the pathogenic species and serotypes of suspected infectious pleuropneumonia in a pig farm in Luoyang,and to provide a reference plan for its clinical scientific epidemic prevention and rational drug use,in this experiment,the diseased pig lungs and other diseased materials were collected aseptically,and the pathogenic bacteria were isolated and identified in laboratory by morphological observation,Gram staining,physiological and biochemical characteristics test,PCR diagnosis and other technologies.It was confirmed by gene sequencing and homology analysis.The pathogenicity and drug resistance of the isolated strains were analyzed by animal attack test and drug sensitivity test.Five pairs of primers were used for genotyping to determine the serotypes.The results showed that the colony morphology of 2007 was smooth,transparent,orderly,round and protuberant,Gram staining was negative and polytypic.It had the typical culture characteristics of Actinobacillus pleuropneumoniae (APP),and the biochemical results were consistent with the biochemical characteristics of Actinobacillus pleuropneumoniae.The results of homology analysis showed that the homology between the isolate 2007 and Actinobacillus pleuropneumoniae strain FJ848573.1 was 99.4%,indicating that the isolate 2007 was Actinobacillus pleuropneumoniae.The results showed that the isolate 2007 had strong pathogenicity to mice.The results of drug sensitivity test showed that the isolate 2007 was highly sensitive to temicillin,ampicillin and ceftiofur sodium,completely resistant to 20 kinds of antimicrobial agents,such as tilmicosin,erythromycin and florfenicol,and the resistance rate to 34 kinds of antibiotics was 76.5%,with strong multiple drug resistance.The results of the isolate 2007 genotyping showed that the target gene fragment was consistent with serotype 5.The results of this study provided specific prevention and treatment measures for the pig farms,and finally achieved good results,which provided some references for the establishment of clinical resistant fold points of Actinobacillus pleuropneumoniae.

Key words: porcine contagious pleuropneumonia; isolation and identification; genotyping; drug sensitivity

CLC Number:

S852.61⁺9

LI Kaiming, DONG Faming, LIU Shouchuan, LIU Jie, LIANG Liqin, LI Kaiqiang. Isolation and Identification of Porcine Contagious Actinobacillus pleuropneumoniae in Luoyang[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1241-1249.

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Isolation and Identification of Porcine Contagious Actinobacillus pleuropneumoniae in Luoyang

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