竹鼠源绿色气球菌的分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2020.08.034

Abstract

Abstract: This experiment was aimed to find out the cause of death of Rhizomys sinensis in a Rhizomys sinensis farm in Guizhou.A strain of bacteria (GZ-S1) was isolated from the submitted Rhizomys sinensis in this study,which was identified through morphological observation,biochemical test,16S rRNA gene sequence analysis,antibiotic sensitivity tests,resistance genes detection and animal infection experiment.The results showed that the isolates was Gram-positive with α-hemolysis reaction and could grow smooth and white colonies on the blood plate.Biochemical identification showed that the isolates had positive reactions to sucrose,fructose,glucose,nitrate reduction,indigo matrix and citrate,but negative reactions to galactose,mannose,hydrogen sulfide test,VP test,MR test and lysine decarboxylase contact test.After 16S rRNA gene sequencing,BLAST alignment and phylogenetic tree analysis,it was found that the isolates GZ-S1 was on the same branch as 12 reference strains of the balloon strain 201707CJKOP-Y31 (MG593595.1),Aerococcus viridans strain Mnlv2 (GQ246745.1),and so on.The results of bacterial drug sensitivity test showed that the isolates were sensitive to oxacillin, carbenicillin and ceftriaxone, was moderate sensitivity to amoxicillin,ofloxacin,sulfamethoxazole,bacteresulf polymyxin B,florfenicol and cefradine, and was resistant to enrofloxacin, kanamycin, tobramycin, neomycin, doxycycline and tetracycline.The result of resistance genes detection showed that the isolates carried resistance genes including tetA(1 031 bp),tetB(513 bp),tetD(326 bp),aadd(146 bp) and aac(6')Ⅰb(655 bp),which was consistent with the antibiotic sensitivity phenotypes.The results of artificial infection test showed that the isolates had pathogenicity.This study made an accurate diagnosis of the causes of the disease in a Rhizomys sinensis farm,and provided a reference for the prevention and rational drug use of bacterial diseases for Rhizomys sinensis.

Key words: Rhizomys sinensis; isolation and identification; Aerococcus viridans; 16S rRNA gene; drug sensitivity; drug resistance gene

CLC Number:

S852.61⁺1

YAN Chaohua, WEN Ming, ZHOU Bijun, ZHANG Xu, CHEN Guoquan, ZHAO Dajie, ZHANG Tiantian, WANG Na, LIU Yanhan. Isolation,Identification and Drug Resistance Analysis of Aerococcus viridans from Rhizomys sinensis[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2643-2651.

References

[1] 秦毅斌,何苹萍,卢冰霞,等.猪源棒状杆菌的分离及其16S rRNA基因分析[J].畜牧与兽医,2016,48(3):95-99. QIN Y B,HE P P,LU B X,et al.Isolation and 16S rRNA gene analysis of Corynebacterium suis[J].Animal Husbandry and Veterinary,2016,48(3):95-99.(in Chinese)
[2] WILLIAMS R E O,HIRCH A,COWAN S T.Aerococcus,a new bacterial genus[J].Journal of General Microbiology,1953,8(3):475-480.
[3] WILSON JR G E,JACOB G S,SCHAEFER J.Solid-state 15N NMR studies of the effects of penicillin on cell-wall metabolism of Aerococcus viridans (Gaffkya homari)[J].Biochemical and Biophysical Research Communications,1985,126(3):1006-1012.
[4] FACKLAM R,ELLIOTT J A.Identification,classification,and clinical relevance of catalase negative,Gram positive cocci[J].Excluding the Streptococci and Enterococci,1995,8(4):479-495.
[5] 林华,陈世界,杨苗,等.1株猪源绿色气球菌的分离鉴定[J].畜牧与兽医,2015,47(8):113-116. LIN H,CHEN S J,YANG M,et al.Isolation and identification of a strain of Aerococcus viridans from pigs[J].Animal Husbandry & Veterinary Medicine,2015,47(8):113-116.(in Chinese)
[6] QING Z,CHRISTOPHER K,SILVIA A,et al.Aerococcus urinae in urinary tract infections[J].Journal of Clinical Microbiology,2000,38(4):1703-1705.
[7] PA L,FALSENE T J,COLLINSM D.Aerococcus sanguicola sp.nov.isolated from a human clinical source[J].International Journal of Systematic & Evolutionary Microbiology,2001,51(2):475-479.
[8] CATTOIR V,KOBAL A,LEGRAND P,et al.Aerococcus urinae and Aerococcus sanguinicola,two frequently misidentified uropathogens[J].Scandinavian Journal of Infectious Diseases,2010,42(10):775-780.
[9] FACKLAM R,LOVGREN M,SHEWMAKER P L,et al.Phenotypic description & antimicrobial susceptibilities of Aerococcus sanguinicola isolates from human clinical samples[J].Journal of Clinical Microbiology,2005,41(6):2587-2592.
[10] 刘洁,刘梦龙,曲久燕,等.猪源绿色气球菌的分离与鉴定[J].中国猪业,2019,14(6):26-30. LIU J,LIU M L,QU J Y,et al.Isolation and identification of Aerococcus viridans from pigs[J].China Pig Industry,2019,14(6):26-30.(in Chinese)
[11] 徐越.奶牛隐性乳腺炎气球菌的分离鉴定及其致病性和耐药性分析研究[D].杨凌:西北农林科技大学,2019. XU Y.Isolation,identification,pathogenicity and drug resistance of Aerococcus occult mastitis in dairy cattle[D].Yangling:Northwest University of A&F Science and Technology,2019.(in Chinese)
[12] MORENO C,ROMERO J,ESPE J O,et al.Polymorphism in repeated 16S rRNA genes is a common property of type strains and environmental isolates of the genus Vibrio[J].Microbiology,2003,148(4):1233-1239.
[13] POPESCU G A,BENEA E,MITACHE E,et al.An unusual bacterium,Aerococcus viridans,and four cases of infective endocarditis[J].The Journal of Heart Valve Disease,2005,14(3):317-319.
[14] SAMUELSSON S,KENNERGREN C,RASMUSSEN M.A case of pacemaker endocarditis caused by Aerococcus urinae[J].Case Reports in Infectious Diseases,2018,2018:253-256.
[15] 董文龙,王巍,勾长龙,等.6株猪源绿色气球菌的分离鉴定及耐药性分析[J].中国兽医学报,2017,37(12):2256-2259. DONG W L,WANG W,GOU C L,et al.Isolation,identification and drug resistance analysis of six strains of Aerococcus viridans from pigs[J].Chinese Journal of Veterinary Medicine, 2017,37(12):2256-2259.(in Chinese)
[16] 李楠,刘晔,张守峰,等.一株山羊源浅绿色气球菌的分离[J].黑龙江畜牧兽医,2015,18:109-111. LI N,LIU Y,ZHANG S F,et al.Isolation of a strain of goat-derived Aerococcus viridans[J].Heilongjiang Animal Science and Veterinary Medicine,2015,18:109-111.(in Chinese)
[17] 张乐宜,李艳,蔡汝健,等.猪源绿色气球菌的分离鉴定及16S rRNA基因序列测定与遗传进化分析[J].中国农学通报,2014,30(29):15-21. ZHANG L Y,LI Y,CAI R J,et al.Isolation and identification of Aerococcus viridans from swine and phylogenetic analysis of 16S rRNA gene sequence[J].China Agronomy Bulletin,2014,30(29):15-21.(in Chinese)
[18] 李浩欣,段纲,项勋,等.仔猪绿色气球菌的分离与鉴定[J].中国兽医学报,2015,35(12):1954-1958. LI H X,DUAN G,XIANG X,et al.Isolation and identification of Aerococcus viridans in piglets[J].Chinese Journal of Veterinary Medicine,2015,35(12):1954-1958.(in Chinese)
[19] EZECHUKWU I,SINGAL M,IGBINOSA O.Aerococcus viridans:Case report,microbiology,and literature review[J].The American Journal of Case Reports,2019,20(7):223-225.
[20] CLARK K F,WADOWSKA D,GREENWOOD S J,et al.Aerococcus viridans var.homari:The presence of capsuleand the relationship to virulence in American lobster (Homarus americanus)[J].Journal of Invertebrate Pathology,2016,133:20-26.
[21] 王苏建,吕达嵘,王家平,等.临床常见绿色气球菌的分布及耐药性分析[J].检验医学,2004,3:190-193. WANG S J,LYU D R,WANG J P,et al.Distribution and drug resistance analysis of common clinical Aerococcus viridans[J].Laboratory Medicine,2004,3:190-193.(in Chinese)
[22] 谢彬,袁洁,袁书智,等.猪源绿色气球菌的分离鉴定与药敏特性研究[J].中国微生态学杂志,2010,22(3):238-243. XIE B,YUAN J,YUAN S Z,et al.Isolation and identification of Aerococcus viridans from swine and research on its susceptibility characteristics[J].Chinese Journal of Microbiology,2010,22(3):238-243.(in Chinese)
[23] 黄兴利,王延峰.肝脓肿患者中检出绿色气球菌1例[J].山东医药,2003,35:16. HUANG X L,WANG Y F.1 case of Aerococcus viridans aeruginosa in patients with liver abscess[J].Shandong Medical Journal,2003,35:16.(in Chinese)
[24] 孙化露,陈冰,李树纯,等.7种鸭源细菌的分离与16S rDNA测序鉴定[J].中国兽医杂志,2011,47(10):13-16. SUN H L,CHEN B,LI S C,et al.Isolation and 16S rDNA sequencing of seven duck derived bacteria[J].Chinese Journal of Veterinary Medicine,2011,47(10):13-16.(in Chinese)
[25] 可小丽,卢迈新,黎炯,等.罗非鱼绿色气球菌的鉴定及致病性研究[J].水生生物学报,2011,35(5):796-802. KE X L,LU M X,LI J,et al.Identification and pathogenicity of Aerococcus viridans in tilapia[J].Journal of Aquatic Biology,2011,35(5):796-802.(in Chinese)

Isolation,Identification and Drug Resistance Analysis of Aerococcus viridans from Rhizomys sinensis

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