鲤鱼白细胞介素10基因组DNA克隆及序列分析

Abstract

Abstract: The purpose of this study was to obtain the full-length sequence of common carp interleukin 10(IL-10) genomic DNA. The sequence of common carp IL-10 full-length cDNA(GenBank:JX 524550) was used. In this study, we designed two pairs of primers in its untranslated regions. Using the carp spleen genomic DNA as the template, we carried out PCR and obtained the IL-10 full-length genomic DNA sequence. The sequence was 2176 bp in length, and the GenBank accession number was JX524551. We compared the gained sequence with the other IL-10 and found their similarity of all harboring 5 exons and 4 introns. Moreover, all exons were comparatively conserved. The exons and introns splice sites were in accordance with "gt......ag" rules. Our results indicated that it was the sizes of introns that were crucial in the process of evolution.

Key words: common carp; interleukin 10; genome; cloning; sequence analysis

CLC Number:

Q785

FENG Xiang-ru, CHEN Yi-long, SUN Zhen, JIA Sheng-mei, WANG Wen-dong, ZHANG Jun-hui, YANG Zhen-guo, LU Qiang. Cloning and Sequence Analysis of Interleukin 10 Genomic DNA in Common Carp (Cyprinus carpio L.)[J]. , 2013, 40(4): 65-68.

References

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Cloning and Sequence Analysis of Interleukin 10 Genomic DNA in Common Carp (Cyprinus carpio L.)

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