1株猪源动物联合乳杆菌S7全基因组测序及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2025.01.003

Abstract

Abstract: 【Objective】 The aim of this study was to sequence the whole genome and analyze the bioinformatics of Ligilactobacillus animalis S7,a strain isolated from pig with good acid tolerance and bile salt tolerance. 【Method】 The sequencing technology combining the third generation PacBio RS Ⅱ sequencing platform and the second generation Illumina HiSeq 2000 sequencing platform was used to sequence the genome of S7,and then the functional genes of the genome were annotated in the databases of GO,EggNOG,KEGG,CAZy,VFDB and CARD.The phylogenetic tree was constructed used TYGS and collinearity analysis was performed with two nearest type strains. 【Result】 The genome size of Ligilactobacillus animalis S7 was 2.03 Mb,and the GC content was 44.14%.A total of 1 993 encoding genes were predicted,including 65 tRNA,19 rRNA,35 sRNA,29 housekeeping genes,11 gene isolands,6 prophages,4 CRISPR-Cas,8 insertion sequences and 10 transposons.1 521,1 567,1 185,and 60 genes were annotated in the databases of GO,EggNOG,KEGG and CAZy,respectively.181 virulence genes and 110 drug resistance genes were annotated in VFDB and CARD databases.In addition,14 acid tolerance-related genes,2 bile salt tolerance-related gene,22 cell adhesion and aggregation-related genes were found in S7.A variety of genes related to heat stress,oxidative stress resistance,and bacteriocin synthesis that are associated with probiotic functions were also found in the genome of S7.The phylogenetic tree and collinearity analysis results indicated that the strain had the nearest evolutionary relationship to type strain Ligilactobacillus animalis P38. 【Conclusion】 The genome of Ligilactobacillus animalis S7 had functional genes related to acid tolerance,bile salt tolerance,adhesion and aggregation,temperature stress,oxidative stress,and bacteriocin,which provided theoretical basis for the scientific application of the strain in feed additives.

Key words: pig; Ligilactobacillus animalis; whole genome sequencing; gene annotation

CLC Number:

S852.6

LIU Hui, JI Haifeng, WANG Sixin, CHEN Meixia, ZHANG Dongyan. Whole Genome Sequencing and Bioinformatics Analysis of Ligilactobacillus animalis S7 from Pig[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 25-38.

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Whole Genome Sequencing and Bioinformatics Analysis of Ligilactobacillus animalis S7 from Pig

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