China Animal Husbandry and Veterinary Medicine ›› 2025, Vol. 52 ›› Issue (1): 80-93.doi: 10.16431/j.cnki.1671-7236.2025.01.008

• Nutritionand Feed • Previous Articles    

Effect of Dietary Addition of Rosa roxburghii Tratt. Residue on Intestinal Histomorphology,Antioxidant Capacity,Immunity and Metabolomics in Common Carp (Cyprinus carpio)

FENG Yi1, YANG Yunyun1, LUO Yongrong2, WU Peng1, WANG Yan1, YANG Ying1, CHEN Jiangfeng1, JIANG Haibo1, WEN Ming1,3   

  1. 1. College of Animal Science, Guizhou University, Guiyang 550025, China;
    2. Institute of Animal Husbandry, Aquaculture and Veterinary Medicine, Anshun Academy of Agricultural Sciences, Anshun 561000, China;
    3. Guizhou Engineering and Technology Research Center of Animal Biological Products, Guiyang 550025, China
  • Received:2024-04-23 Published:2024-12-30

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Abstract: 【Objective】 This study was aimed to investigate the effect of adding Rosa roxburghii Tratt. residue to the diet on the intestinal histomorphology,antioxidant capacity,immunity and metabolomics of common carp,so as to provide a scientific basis for the popularization and application of Rosa roxburghii Tratt. residue feed for paddy-fish farming in rural mountainous areas of Guizhou province. 【Method】 One hundred and eighty healthy and disease-free common carp with an initial body weight of 25 g±1 g were selected and randomly divided into normal group (no Rosa roxburghii Tratt. residue was added to basal feed) and Rosa roxburghii Tratt. residue feeding group (0.4% Rosa roxburghii Tratt. residue was added to basal feed),with three replicates of 30 fish in each group,the experiment period was 60 d.The mid-portion of the intestine of common carp was collected after the experiment,and the intestinal histomorphology,antioxidant capacity,immunity and metabolomics of common carp were analyzed by microscopic observation,ELISA and metabolomics technology based on liquid chromatography. 【Result】 Compared with normal group,the feeding of Rosa roxburghii Tratt. residue had no adverse effect on the normal structure of intestine in common carp,the length of intestinal villi and thickness of muscularis propria were both extremely significantly increased (P＜0.01),the activity of glutathione peroxidase (GSH-Px) in intestinal tract of common carp in Rosa roxburghii Tratt. residue feeding group was significantly increased (P＜0.05),and the content of tumor necrosis factor-α (TNF-α) was significantly decreased (P＜0.05).A total of 85 differential metabolites were significantly enriched in Rosa roxburghii Tratt. residue feeding group,including 2-ketobutyric acid,L-(+)-lactic acid,propionic acid,succinic acid,D-aspartic acid,D-glutamine,xanthine nucleoside,hypoxanthine,thymine,uracil,D-pyroglutamic acid,etc.,which were mainly enriched in the metabolic pathways of propionate metabolism,alanine,aspartate and glutamate metabolism,purine metabolism,pyrimidine metabolism,etc. 【Conclusion】 The addition of Rosa roxburghii Tratt. residue in diet could improve the antioxidant property and immune indexes of intestinal tissue in common carp,which might improve intestinal health and enhance immunity of common carp by affecting the purine and pyrimidine metabolism of common carp as well as the related metabolites,such as D-glutamine,xanthine nucleoside,hypoxanthine,thymine,2'-deoxyuridine-5'-monophosphate and uracil.

Key words: Rosa roxburghii Tratt. residue; common carp; intestinal histomorphology; antioxidant capacity; immunity; metabolomics

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