3个山羊品种角蛋白辅助蛋白7基因的遗传多态性分析

Abstract

Abstract: The polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was used to detect the polymorphism of keratin associated protein 7 (KAP7) in Shanbei White cashmere goat,Taihang Black goat and Nanjiang White cashmere goat. The results showed that the KAP7 presented AA and AB genetypes in these three breeds,AA genetype was the dominant genetypes. The PCR production of different genotype of KAP7 were purified and sequnenced. One mutation,129 bp(C→G) was found in KAP7. This mutation site was in 5' regulatory region.

Key words: KAP7 gene; PCR-SSCP; Shanbei White cashmere goat; Taihang Black goat; Nanjiang White cashmere goat

CLC Number:

S827

PENG Yi-yi, YANG Zhao-xia, GENG Rong-qing, CHEN Yu-lin, ZHANG Fu-quan, ZHANG Xi-shun. Genetic Polymorphism Analysis of KAP7 Gene in 3 Goat Breeds[J]. , 2013, (7): 133-136.

References

1 尹俊, 扈庭茂, 李金泉, 等. 内蒙古绒山羊毛囊兴盛期皮肤cDNA文库的构建及KAP6-2 cDNA的克隆[J]. 动物学研究, 2004, 25(2): 166~171.
2 王杰, 代怡婧, 王永, 等. 高原型藏山羊KAP 6.1 和6.2位点与产绒性状的关系研究[J]. 西南民族大学学报(自然科学版), 2010, 36(6):962~965.
3 王志有, 祁全青, 陈玉林. 高原型藏绵羊KAP基因单核苷酸多态性分析[J]. 中国畜牧兽医, 2010, 37(10): 120~124.
4 汪玲, 袁嵩雪, 邢梦欣, 等. 绒山羊KAP基因与遗传育种关系的研究进展[J]. 安徽农学通报, 2010, 16(5): 62~64.
5 赵俊星, 任有蛇, 岳文斌. 3个山羊品种KAP6.2和KAP7基因的PCR-SSCP分析[J]. 中国畜牧兽医, 2007, 34(12):42~45.
6 张亚妮, 张恩平, 陈玉林, 等. KAP 基因的多态性与辽宁绒山羊经济性状的关系研究[J]. 中国农业科学, 2007, 40(9): 2062~2067.
7 耿社民, 常洪, 秦围庆, 等. 绒山羊结构基因标记座位与经济性状关系的研究[J]. 畜牧兽医学报, 2003, 34(1): 24~27.
8 Itenge-Mweza T O, Forrest R H, Mckenzie G W, et al. Polymorphism of the KAP1.1, KAP1.3 and KAP33 genes in Merino sheep[J]. Molecular and Cellular Probes, 2007, 21(5~6): 338~342.
9 Jin M, Wang L, Li S, et al. Characterization and expression analysis of KAP7.1, KAP8.2 gene in Liaoning new-breeding cashmere goat hair follicle[J]. Molecular Biology Reports, 2011, 38(5):3023~3028.
10 Kazunori S, Jun K, Izumi O, et al. Comparative genomics of the keratin-associated protein (KAP) gene clusters in human, chimpanzee, and baboon[J].Mammalian Genome,2004,15(3):179~192.
11 McLaren R J, Rogers G R, Davies K P, et al. Linkage mapping of wool keratin and keratin associated protein genes in sheep[J].Mammalian Genome, 1997,8(12):938~940.
12 Rogers G R, Hickford J G H, Bickerstaffe R, et al. Polymorphism in two genes for B2 high sulfur proteins of wool[J]. Animal Genetics, 1994, 25(6): 407~415.
13 Purvis I W, Franklin I R. Major genes and QTL influencing wool production and quality[J]. Genetics Selection Evolution, 2005, 37(Suppl.1): S97~S107.

[1]	ZHANG Yonghao, XIAO Yi, GUO Lijun, Tuerxun·Abudoureheman, WANG Yutao. Genetic Polymorphism Analysis of MHC-DRB3 Gene Exon 2 in Ovis ammon polii and Five Domestic Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 962-972.
[2]	YANG Xiaobin, HE Lili, ZHANG Yang, ZHOU Ruifeng, HOU Kangli, ZHANG Yunyun, WANG Yueli, LI Qingqing, SHI Bingang, HU Jiang. Polymorphisms of LF Gene and Their Association with Milk Quality Traits in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1655-1663.
[3]	ZHANG Fan, XU Jie, LI Gang, ZHOU Yongshun, HE Ying, HAN Kai, CHEN Fulong, GAO Jianfeng. Correlation Analysis of iNOS Gene Polymorphism and Brucellosis in Kazakh Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1102-1111.
[4]	LIU Haixia, HAN Dayong, ZHU Aiwen, YAN Wei, WANG Buzhong, CAI Zijian. Effect of the Polymorphism of GnRHR and INHA Genes and Its Interaction on Litter Size in Wuhu Hybrid Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 780-788.
[5]	MA Xuezhen, XU Jie, GAO Jianfeng, LI Gang. The Correlation Between Polymorphisms of Exon1 and Exon4 of DRB1 Gene and Brucellosis in Kazakh Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3844-3851.
[6]	LUO Cheng, WANG Yuanyuan, WANG Huimin, TAN Jun, ZHOU Guangpu, GAO Jianfeng. Polymorphism of TLR2 Gene in Chinese Merino Sheep and Its Correlation Analysis with Brucellosis [J]. , 2018, 45(5): 1145-1152.
[7]	SHI Lei, QU Lei, LIU Jinwang, ZHU Haijing, LI Longping, HUANG Shuai. Hair Follicle Structure and the Correlation Analysis Between Characteristic Parameters and Cashmere Production Traits in Shanbei White Cashmere Goat [J]. , 2018, 45(10): 2814-2822.
[8]	BAI Jun-yan, YANG Shuai, PANG You-zhi, WANG Xu, SUN Gang. Polymorphism Analysis of the Agouti-related Protein Gene Exon Ⅰof Quail [J]. , 2017, 44(5): 1425-1430.
[9]	ZHU Ai-wen, ZHOU Chun-bao, NI Li-gang, HAN Da-yong. The Association Analysis Between the SNPs of Leptin Gene and Litter Size Trait in Pigs [J]. , 2017, 44(4): 1122-1127.
[10]	WANG Yuan-yuan, YAN Guo, LUO Cheng, QI Jiang-jiao, ZHOU Guang-pu, TAN Jun, GAO Jian-feng. Polymorphisms and Its Genetic Variation Analysis of DQB2 Gene Exon 2 in Kazakh Sheep [J]. , 2017, 44(12): 3543-3553.
[11]	ZHANG Chun-lei, ZHOU Yuan, FANG Xing-tang, HOU Shui-sheng, ZHANG Gui-ying, CHEN Hong, CHEN Guo-hong. Polymorphism of PRKAG2 Gene and its Association Analysis with Economic Traits in Peking Duck [J]. , 2016, 43(4): 1045-1049.
[12]	WANG Wen-wen, XU Wan-yun, HU Meng-wei, LI Jian-hua, LIU Peng-tao, GAO Jian-feng. The Correlation Analysis between Chinese Merino Sheep OLA-DQB1 Gene Exon 2 Genetic Polymorphism and Brucellosis Susceptibility [J]. , 2015, 42(6): 1495-1503.
[13]	CHEN Sheng-mei, LUO Hong, NIU Jian-zhang, MA Yu-hong, CHAI Zhi-xin, MA Zhi-jie, ZHONG Jin-cheng. Study on Polymorphism of 5'-Flank Region of FSHR Gene in Yak and Cattle-yak [J]. , 2015, 42(4): 980-985.
[14]	LIU Hong-li, XU Ya-ou, XIONG Bin, HU Wei-min, MEI Han, SHI Hao, ZUO Bin, NIE Xiao-qing, WANG Hai. Study on the Correlation between Polymorphisms of MyoG Gene and Growth Traits in Luning Chicken [J]. , 2015, 42(3): 629-637.
[15]	XU Wan-yun, WANG Hui-min, LI Jian-hua, WANG Wen-lun, HU Meng-wei, GAO Jian-feng. The Polymorphisms of Chinese Merino Sheep eNOS Gene exon8 and its Association with Brucellosis Susceptibility [J]. , 2015, 42(12): 3294-3299.

Genetic Polymorphism Analysis of KAP7 Gene in 3 Goat Breeds

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments