›› 2012, Vol. 39 ›› Issue (7): 53-58.

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Effects of Different Sites of Base-pair Substitution on Calibration of Anatidae Molecular Clocks

MENG Dong-mei   

  1. Department of Pharmaceutical Engineering, Heze University, Heze 274015, China
  • Received:2011-12-06 Online:2012-07-20 Published:2012-07-16

Abstract: In order to estimate the divergence times of Anatidae and investigate their genetic background, the mitochondrial cytochrome b (Cyt b) gene partial sequences provided at NCBI were totally constructed a 13 sequences database for the next step analyze. The divergence time between Anseriformes and Galliformes was chosen as an anchor point to calibrate the Anatidae molecular clocks using a sequence calibration method. The results showed that the estimation of the divergence time was 116.3785MYA("1+2TiTv") between Phasianidae and Anatidae.The estimation of divergence time was 63.8823MYA("1+2TiTv") between Anser and Anatinae. A divergence of Gallus and Coturnix occurred in 1.879331MYA("1+2TiTv").The estimated divergence time of Anser anser and Anser cygnoidesa was 37.6708MYA("1+2TiTv").The model of "1+2TiTv" was one kind of ideal model.The divergence of the two basal groups(Phasianidae/Anatidae, Anser/Anatinae)took place in the Cretaceous (137-65 MYA). The results were consistent with the view that avian was originated in the Cretaceous.

Key words: molecular clock; cytochrome b (Cyt b); Anatidae; divergence time

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