长链非编码RNA的调控机制及其在家畜中的预测方法

doi:10.16431/j.cnki.1671-7236.2017.07.001

Abstract

Abstract:

Long noncoding RNA (lncRNA)is a functional RNA segment longer than 200 nucleotides,without protein-coding capacity.lncRNAs can directly regulate gene expression in the form of RNA,at epigenetic, transcription,post-transcription,translation and post-translation level,also plays important roles in various biological processes in the body of livestock.The author reviewed origin,classifications and structural features, four kinds of molecular regulation mechanisms for implement biological functions (signal moleculars,decoy moleculars,guide moleculars,scaffold molecules),five levels of regulation gene expression of lncRNA,and the application of high-throughput RNA-Seq technique to analyze the sequencing data and introduce the methods for forecasting livestock lncRNA,provided a basis for the reasonable study of livestock lncRNA.Predition of the livestock lncRNA includes four sections:Deep sequencing, transcriptome reconstruction,new transcription forecast and lncRNA identification.At the stage of lncRNA identification,the author's laboratory adopt coding potential assessment tool and online sequence coding potential calculator intersection method to remove the coding potential transcript from a large of the predicting candidates rapidly,also got 68 604 lncRNA of the skin tissues of Inner Mongolia cashmere goats,enriched the basic data of goat lncRNA transcript in the NONCODE database.

Key words: long noncoding RNAs (lncRNA); regulatory; forecasting methods; CPAT; CPC

CLC Number:

Q756

JIA Chun-yan, JI Xiao-yang, BAI Xue, DAI Hao-yang, WANG Jian-meng, ZHANG Wen-guang. Regulatory Mechanisms of Long Noncoding RNAs and Forecasting Methods in Livestock[J]. , 2017, 44(7): 1895-1905.

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Regulatory Mechanisms of Long Noncoding RNAs and Forecasting Methods in Livestock

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